Cited by 279Cellular transport and secretionNeuroscience and Neuropharmacology ResearchExtracellular vesicles in disease

Single-vesicle imaging reveals that synaptic vesicle exocytosis and endocytosis are coupled by a single stochastic mode

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Balaji, J., Ryan, T. A.·2007·Proceedings of the National Academy of Sciences

Abstract

The nature of synaptic vesicle recycling at nerve terminals has been a subject of considerable debate for >35 years. Here, we report the use of an optical strategy that allows the exocytosis and retrieval of synaptic components to be tracked in real time at single-molecule sensitivity in living nerve terminals. This approach has allowed us to examine the recycling of synaptic vesicles in response to single action potentials. Our results show that, after exocytosis, individual synaptic vesicles are retrieved by a stochastic process with an exponential distribution of delay times, with a mean time of approximately 14 s. We propose that evidence for fast endocytosis, such as that proposed to support the presence of kiss-and-run, is likely explained by the stochastic nature of a slower process.

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Crossref · 10.1073/pnas.0707574105

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Authors
Balaji, J., & Ryan, T. A.
Affiliations
Weill Cornell Medicine (US)
Venue
Proceedings of the National Academy of Sciences
Published
2007-12-18
Volume
104
Issue
51
Pages
20576-20581
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2008643711
Cited by
279
References
24
Citations by year
2026: 5 · 2025: 12 · 2024: 11 · 2023: 7 · 2022: 18 · 2021: 12 · 2020: 14 · 2019: 17 · 2018: 18 · 2017: 19 · 2016: 12 · 2015: 14 · 2014: 23 · 2013: 20 · 2012: 10
FWCI
7.01
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • University of California, San Francisco
  • National Institute of Neurological Disorders and Stroke (R01NS036942)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
synaptic vesicle recycling, exocytosis, endocytosis, kiss-and-run, single-vesicle imaging, single-molecule tracking, presynaptic terminals, synaptic transmission
MeSH terms
Animals, Cells, Cultured, Endocytosis, Exocytosis, Microscopy, Fluorescence, Synaptic Vesicles, Rats, Sprague-Dawley, Photobleaching, Green Fluorescent Proteins, Rats

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