Pericytes from mesenchymal Stem cells as a model for the blood-brain barrier
Open accessAbstract
Blood brain-barrier (BBB) in vitro models have been widely reported in studies of the BBB phenotype. However, established co-culture systems involve brain endothelial cells, astrocytes, neurons and pericytes, and therefore are often consuming and technically challenging. Here we use mesenchymal system cells (MSC) as a potential substitute for pericytes in a BBB model. Both MSC and pericyte markers in 2D culture environment were evaluated on different extracellular matrix compositions. Further experiments indicated that MSC contributed in a similar manner to pericytes in a co-cultured 3D model on increasing trans-endothelial electric resistance (TEER) and decreasing permeability against macromolecules.
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Co-culture with MSCs has been shown to increase TEER and decrease permeability in endothelial cells.
“Co-culture with MSCs have been shown to increase TEER and decrease permeability in endothelial cells (Tian et al., 2017).”
From APOE3-Christchurch variant enhances neurovascular support functions of iPSC-derived mesenchymal stromal cells (2026) · cites this paper as Tian et al., 2017
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Alimperti, S., Mirabella, T., Bajaj, V., Polacheck, W., Pirone, D. M., Duffield, J., Eyckmans, J., Assoian, R. K., & Chen, C. S. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1618333114 · Cited by 122 · see citing works
Rodriguez Martinez, P. J., Yslas, A. R., Inoue, Y., Parsons, T. M., Baker, S. K., Lu, W., Raulin, A.-C., & Kanekiyo, T. (2026) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2026.1778856
- Authors
- Tian, X., Brookes, O., & Battaglia, G.
- Affiliations
- Anhui University (CN)
- Queen Mary University of London (GB)
- University College London (GB)
- Corresponding author
- Tian, Xiaohe
- Battaglia, Giuseppe
- Venue
- Scientific Reports
- Published
- 2017-01-18
- Volume
- 7
- Issue
- 1
- Pages
- 39676-39676
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5241806
- OpenAlex
- W2573641388
- Cited by
- 56
- References
- 0
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 6 · 2023: 6 · 2022: 6 · 2021: 8 · 2020: 8 · 2019: 7 · 2018: 8 · 2017: 1
- FWCI
- 3.27
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Queen Mary Research Online (Queen Mary University of London) (submittedVersion, cc-by)
- Funders
- King's College London
- National Natural Science Foundation of China (21602003)
- Engineering and Physical Sciences Research Council (EP/G062137/1, EP/I001697/1)
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- blood-brain barrier, mesenchymal stem cells, pericytes, in vitro BBB model, co-culture, extracellular matrix, transendothelial electrical resistance, barrier permeability, 3D cell culture
- MeSH terms
- Animals, Blood-Brain Barrier, Extracellular Matrix, Mice, Inbred C57BL, Models, Neurological, Permeability, Coculture Techniques, Cell Culture Techniques, Pericytes, Mesenchymal Stem Cells
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