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Cited by 52Osteoarthritis Treatment and MechanismsRNA modifications and cancerBone Metabolism and Diseases

Serum snoRNAs as biomarkers for joint ageing and post traumatic osteoarthritis

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Steinbusch, Mandy M. F., Fang, Yongxiang, Milner, Peter I., Clegg, Peter D., Young, David A., Welting, Tim J. M., Peffers, Mandy J.·2017·Scientific Reports
DOI 10.1038/srep43558PMC PMC5333149
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Abstract

The development of effective treatments for the age-related disease osteoarthritis and the ability to predict disease progression has been hampered by the lack of biomarkers able to demonstrate the course of the disease. Profiling the expression patterns of small nucleolar RNAs (snoRNAs) in joint ageing and OA may provide diagnostic biomarkers and therapeutic targets. This study determined expression patterns of snoRNAs in joint ageing and OA and examined them as potential biomarkers. Using SnoRNASeq and real-time quantitative PCR (qRT-PCR) we demonstrate snoRNA expression levels in murine ageing and OA joints and serum for the first time. SnoRNASeq identified differential expression (DE) of 6 snoRNAs in young versus old joints and 5 snoRNAs in old sham versus old experimental osteoarthritic joints. In serum we found differential presence of 27 snoRNAs in young versus old serum and 18 snoRNAs in old sham versus old experimental osteoarthritic serum. Confirmatory qRT-PCR analysis demonstrated good correlation with SnoRNASeq findings. Profiling the expression patterns of snoRNAs is the initial step in determining their functional significance in ageing and osteoarthritis, and provides potential diagnostic biomarkers and therapeutic targets. Our results establish snoRNAs as novel markers of musculoskeletal ageing and osteoarthritis.

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  • The authors have not been able to find a report that SNORD115 or individual members of the SNORD115 cluster were released in plasma EV of patients with lung cancer.

    “However, we have not been able to find a report that SNORD115 or individual members of SNORD115 cluster were released in plasma EV of patients with lung cancer (Nossent et al., 2019; Steinbusch et al., 2017).”

    From Small nucleolar RNAs in plasma extracellular vesicles and their discriminatory power as diagnostic biomarkers of Alzheimer's disease (2021) · cites this paper as Steinbusch et al., 2017

    The Results and Discussion address serum snoRNAs in joint ageing and osteoarthritis, particularly SNORD116, but do not address SNORD115 in plasma extracellular vesicles from patients with lung cancer. Searches for SNORD115 and lung-cancer plasma extracellular vesicles found no passage in our copy establishing the citing authors’ statement about the absence of a report. (LLM, conf=0.75)

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Authors
Steinbusch, M. M. F., Fang, Y., Milner, P. I., Clegg, P. D., Young, D. A., Welting, T. J. M., & Peffers, M. J.
Affiliations
  • Maastricht University Medical Centre (NL)
  • University of Liverpool (GB)
  • Newcastle University (GB)
Corresponding author
Steinbusch, Mandy M. F.
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2017-03-02
Volume
7
Issue
1
Pages
43558-43558
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/srep43558
PMC
PMC5333149
OpenAlex
W2592645432
Cited by
52
References
3
Citations by year
2026: 5 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 7 · 2021: 7 · 2020: 8 · 2019: 3 · 2018: 8 · 2017: 3
FWCI
5.26
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (submittedVersion, cc-by)
  • Research Publications (Maastricht University) (submittedVersion, other-oa)
  • Newcastle University ePrints (Newcastle Univesity) (cc-by)
Funders
  • Wellcome Trust (107471, /Z/15/Z, 107471/Z/15/Z)
  • Deutsche Forschungsgemeinschaft
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek
  • Versus Arthritis (19424)
  • Dutch Arthritis Association (LLP14)
  • Medical Research Council (MR/K006312/1, LLP14, 107471)
Topics
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
Keywords
small nucleolar RNA, serum biomarkers, post-traumatic osteoarthritis, differential gene expression, diagnostic biomarkers, therapeutic target
MeSH terms
Cell-Free Nucleic Acids, Animals, Horses, Joints, Male, Osteoarthritis, Reproducibility of Results, Biomarkers, RNA, Small Nucleolar, Gene Expression Profiling, Mice, Transcriptome, Real-Time Polymerase Chain Reaction

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