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Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as alditol acetates
Abstract
A procedure is described for the measurement and characterisation of total non-starch polysaccharides in plant foods by gas-liquid chromatography of individual sugars as alditol acetates. Starch, including that resistant to gelatinisation in boiling water, is dispersed with dimethyl sulphoxide and then hydrolysed with α-amylase and pullulanase. Starch-free material is hydrolysed with sulphuric acid and the released neutral sugars are measured as alditol acetates by using N-methylimidazole in order to catalyse the acetylation. Uronic acids are measured by a spectrophotometric method. The procedure is a modification of a more comprehensive method reported previously. It is relatively rapid and simple compared with gravimetric procedures, is applicable to a wide range of foodstuffs, can be carried out without special expertise in carbohydrate chemistry and is suitable for routine laboratory use.
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Cited by
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Courtin, C. M., & Delcour, J. A. (2002) · Journal of Cereal Science · doi:10.1006/jcrs.2001.0433 · Cited by 720
Fermentation in the human large intestine and the available substrates
Cummings, J. H., & Englyst, H. N. (1987) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/45.5.1243 · Cited by 490
Self-citationColon as a digestive organ in patients with short bowel
Nordgaard, I., Hansen, B. S., & Mortensen, P. B. (1994) · The Lancet · doi:10.1016/s0140-6736(94)91220-3 · Cited by 339
Rowan, A. M., Moughan, P. J., Wilson, M. N., Maher, K., & Tasman-Jones, C. (1994) · British Journal of Nutrition · doi:10.1079/bjn19940108 · Cited by 189
Hald, S., Schioldan, A. G., Moore, M. E., Dige, A., Lærke, H. N., Agnholt, J., Bach Knudsen, K. E., Hermansen, K., Marco, M. L., Gregersen, S., et al. (2016) · PLoS ONE · doi:10.1371/journal.pone.0159223 · Cited by 180
Metadata
- Authors
- Englyst, H. N., & Cummings, J. H.
- Venue
- The Analyst
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 1984
- Volume
- 109
- Issue
- 7
- Pages
- 937
- ISSN
- 0003-2654 (ISSN-L)
- 1364-5528
- OpenAlex
- W2151308351
- Cited by
- 1,088
- References
- 0
- FWCI
- 9.82
- Type
- journal article
- Language
- en
- Open access
- closed
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- Englyst, H. N., & Cummings, J. H. (1984). Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as alditol acetates. The Analyst. https://doi.org/10.1039/an9840900937
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