The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Generation of larger numbers of separated microbial populations by cultivation in segmented-flow microdevices
Abstract
The high speed production of fluid segments for the highly parallelized cultivation of monoclonal cell populations was carried out by the use of microchip segmentor modules. Aqueous fluid segments, embedded in a non-miscible carrier liquid, were produced with frequencies up to 30 s(-1) and showed a high homogeneity in size. This corresponds with the production of about 2.5 million samples per day. The segment volumes can be adapted between about 4 nl and 100 nl. The typical segment size for cultivation experiments is in the range between 40 nl and 80 nl. Nutrient medium can be applied instead of pure water. It is possible to aliquot a cell suspension in such a way that most of the aqueous fluid segments contain only one cell. In model experiments with four microbial species chip-produced aliquots of 60 nl, each containing one or a few cells, were incubated in Teflon capillary tubes. Rapid growth of the microcultures was observed. Cell densities were found to be as high as in conventional shake flask cultures.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Zengler, K., Toledo, G., Rappé, M., Elkins, J., Mathur, E. J., Short, J. M., & Keller, M. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.252630999 · Cited by 796
Cited by
Teh, S.-Y., Lin, R., Hung, L.-H., & Lee, A. P. (2008) · Lab on a Chip · doi:10.1039/b715524g · Cited by 2,690
Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications
Mark, D., Haeberle, S., Roth, G., von Stetten, F., & Zengerle, R. (2010) · Chemical Society Reviews · doi:10.1039/b820557b · Cited by 1,647
Clausell-Tormos, J., Lieber, D., Baret, J.-C., El-Harrak, A., Miller, O. J., Frenz, L., Blouwolff, J., Humphry, K. J., Köster, S., Duan, H., et al. (2008) · Chemistry & Biology · doi:10.1016/j.chembiol.2008.04.004 · Cited by 720
Okushima, S., Nisisako, T., Torii, T., & Higuchi, T. (2004) · Langmuir · doi:10.1021/la0480336 · Cited by 656
Microfluidics technology for manipulation and analysis of biological cells
Yi, C., Li, C.-W., Ji, S., & Yang, M. (2006) · Analytica Chimica Acta · doi:10.1016/j.aca.2005.12.037 · Cited by 478
Metadata
- Authors
- Martin, K., Henkel, T., Baier, V., Grodrian, A., Schön, T., Roth, M., Michael Köhler, J., & Metze, J.
- Venue
- Lab Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2003
- Volume
- 3
- Issue
- 3
- Pages
- 202-207
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2025222636
- Cited by
- 183
- References
- 1
- FWCI
- 5.38
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Generation of larger numbers of separated microbial populations by cultivation in segmented-flow microdevices. https://app.cochat.ai/papers/10.1039/b301258c
- The paper
- Martin, K., Henkel, T., Baier, V., Grodrian, A., Schön, T., Roth, M., Michael Köhler, J., & Metze, J. (2003). Generation of larger numbers of separated microbial populations by cultivation in segmented-flow microdevices. Lab Chip. https://doi.org/10.1039/b301258c
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
