The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device
Abstract
We report a fully integrated, pathogen-specific DNA extraction device utilizing centrifugal microfluidics on a polymer based CD platform. By use of the innovative laser irradiated Ferrowax microvalve (LIFM) together with the rapid cell lysis method using laser irradiation on magnetic particles, we could, for the first time, demonstrate a fully integrated pathogen specific DNA extraction from whole blood on a CD. As a model study, DNA extraction experiments from whole blood spiked with Hepatitis B virus (HBV) and E.coli were conducted. The total process of the plasma separation, mixing with magnetic beads conjugated with target specific antibodies, removal of plasma residual, washing and DNA extraction was finished within 12 min with only one manual step, the loading of 100 microL of whole blood. Real-time PCR results showed that the concentration of DNA prepared on a CD using a portable sample preparation device was as good as those by conventional bench top protocol. It demonstrates that our novel centrifugal microfluidics platform enables a full integration of complex biological reactions that require multi-step fluidic control.
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Cites
The origins and the future of microfluidics
Whitesides, G. M. (2006) · Nature · doi:10.1038/nature05058 · Cited by 9,687
Cited by
Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications
Mark, D., Haeberle, S., Roth, G., von Stetten, F., & Zengerle, R. (2010) · Chemical Society Reviews · doi:10.1039/b820557b · Cited by 1,647
Point of Care Diagnostics: Status and Future
Gubala, V., Harris, L. F., Ricco, A. J., Tan, M. X., & Williams, D. E. (2012) · Analytical Chemistry · doi:10.1021/ac2030199 · Cited by 1,194
Centrifugal microfluidics for biomedical applications
Gorkin, R., Park, J., Siegrist, J., Amasia, M., Lee, B. S., Park, J.-M., Kim, J., Kim, H., Madou, M., & Cho, Y.-K. (2010) · Lab on a Chip · doi:10.1039/b924109d · Cited by 731
Self-citationHang, Y., Boryczka, J., & Wu, N. (2022) · Chemical Society Reviews · doi:10.1039/c9cs00621d · Cited by 377
A fully automated immunoassay from whole blood on a disc
Lee, B. S., Lee, J.-N., Park, J.-M., Lee, J.-G., Kim, S., Cho, Y.-K., & Ko, C. (2009) · Lab on a Chip · doi:10.1039/b820321k · Cited by 302
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Metadata
- Authors
- Cho, Y.-K., Lee, J.-G., Park, J.-M., Lee, B.-S., Lee, Y., & Ko, C.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2007
- Volume
- 7
- Issue
- 5
- Pages
- 565
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2113785346
- Cited by
- 312
- References
- 1
- FWCI
- 13.94
- Type
- journal article
- Language
- en
- Open access
- closed
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- Cho, Y.-K., Lee, J.-G., Park, J.-M., Lee, B.-S., Lee, Y., & Ko, C. (2007). One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device. Lab on a Chip. https://doi.org/10.1039/b616115d
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