The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
A fully automated immunoassay from whole blood on a disc
Abstract
A portable, disc-based, and fully automated enzyme-linked immuno-sorbent assay (ELISA) system is developed to test infectious diseases from whole blood. The innovative laser irradiated ferrowax microvalves and centrifugal microfluidics were utilized for the full integration of microbead-based suspension ELISA assays on a disc starting from whole blood. The concentrations of the antigen and the antibody of Hepatitis B virus (HBV), HBsAg and Anti-HBs respectively, were measured using the lab-on-a-disc (LOD). All the necessary reagents are preloaded on the disc and the total process of the plasma separation, incubation with target specific antigen or antibody coated microbeads, multiple steps of washing, enzyme reaction with substrates, and the absorbance detection could be finished within 30 minutes. Compared to the conventional ELISA, the operation time was dramatically reduced from over 2 hours to less than 30 minutes while the limit of detection was kept similar; e.g. the limit of detection of Anti-HBs tests were 8.6 mIU mL(-1) and 10 mIU mL(-1) for the disc-based and the conventional ELISA respectively.
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Cites
Chaotic Mixer for Microchannels
Stroock, A. D., Dertinger, S. K. W., Ajdari, A., Mezić, I., Stone, H. A., & Whitesides, G. M. (2002) · Science · doi:10.1126/science.1066238 · Cited by 3,435
Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay
Lai, S., Wang, S., Luo, J., Lee, L. J., Yang, S.-T., & Madou, M. J. (2004) · Analytical Chemistry · doi:10.1021/ac0348322 · Cited by 418
One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device
Cho, Y.-K., Lee, J.-G., Park, J.-M., Lee, B.-S., Lee, Y., & Ko, C. (2007) · Lab on a Chip · doi:10.1039/b616115d · Cited by 312
Self-citation(2003) · Lab on a Chip · doi:10.1039/b313217j · Cited by 261
High-sensitivity miniaturized immunoassays for tumor necrosis factor ? using microfluidic systems
Cesaro-Tadic, S., Dernick, G., Juncker, D., Buurman, G., Kropshofer, H., Michel, B., Fattinger, C., & Delamarche, E. (2004) · Lab on a Chip · doi:10.1039/b408964b · Cited by 203
A Microchip-Based Multianalyte Assay System for the Assessment of Cardiac Risk
Christodoulides, N., Tran, M., Floriano, P. N., Rodriguez, M., Goodey, A., Ali, M., Neikirk, D., & McDevitt, J. T. (2002) · Analytical Chemistry · doi:10.1021/ac011150a · Cited by 169
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Microchip-based enzyme-linked immunosorbent assay (microELISA) system with thermal lens detection
Sato, K., Yamanaka, M., Hagino, T., Tokeshi, M., Kimura, H. M., & Kitamori, T. (2004) · Lab on a Chip · doi:10.1039/b411356j · Cited by 112
Bead-based immunoassays using a micro-chip flow cytometer
Holmes, D., She, J. K., Roach, P. L., & Morgan, H. (2007) · Lab on a Chip · doi:10.1039/b707507n · Cited by 105
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Cited by
Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications
Mark, D., Haeberle, S., Roth, G., von Stetten, F., & Zengerle, R. (2010) · Chemical Society Reviews · doi:10.1039/b820557b · Cited by 1,647
Wearable and flexible electronics for continuous molecular monitoring
Yang, Y., & Gao, W. (2019) · Chemical Society Reviews · doi:10.1039/c7cs00730b · Cited by 1,315
Point of Care Diagnostics: Status and Future
Gubala, V., Harris, L. F., Ricco, A. J., Tan, M. X., & Williams, D. E. (2012) · Analytical Chemistry · doi:10.1021/ac2030199 · Cited by 1,194
Centrifugal microfluidics for biomedical applications
Gorkin, R., Park, J., Siegrist, J., Amasia, M., Lee, B. S., Park, J.-M., Kim, J., Kim, H., Madou, M., & Cho, Y.-K. (2010) · Lab on a Chip · doi:10.1039/b924109d · Cited by 731
Self-citationLatest Developments in Micro Total Analysis Systems
Arora, A., Simone, G., Salieb-Beugelaar, G. B., Kim, J. T., & Manz, A. (2010) · Analytical Chemistry · doi:10.1021/ac100969k · Cited by 421
Metadata
- Authors
- Lee, B. S., Lee, J.-N., Park, J.-M., Lee, J.-G., Kim, S., Cho, Y.-K., & Ko, C.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2009
- Volume
- 9
- Issue
- 11
- Pages
- 1548
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W1965041557
- Cited by
- 302
- References
- 10
- FWCI
- 14.75
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lee, B. S., Lee, J.-N., Park, J.-M., Lee, J.-G., Kim, S., Cho, Y.-K., & Ko, C. (2009). A fully automated immunoassay from whole blood on a disc. Lab on a Chip. https://doi.org/10.1039/b820321k
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