Cited by 55Metalloenzymes and iron-sulfur proteinsMetal-Catalyzed Oxygenation MechanismsEnzyme Structure and Function

Molybdenum and tungsten oxygen transferases – structural and functional diversity within a common active site motif

Pushie, M. Jake, Cotelesage, Julien J., George, Graham N.·2014·Metallomics

Abstract

Molybdenum and tungsten are the only second and third-row transition elements with a known function in living organisms. The molybdenum and tungsten enzymes show common structural features, with the metal being bound by a pyranopterin-dithiolene cofactor called molybdopterin. They catalyze a variety of oxygen transferase reactions coupled with two-electron redox chemistry in which the metal cycles between the +6 and +4 oxidation states usually with water, either product or substrate, providing the oxygen. The functional roles filled by the molybdenum and tungsten enzymes are diverse; for example, they play essential roles in microbial respiration, in the uptake of nitrogen in green plants, and in human health. Together, the enzymes form a superfamily which is among the most prevalent known, being found in all kingdoms of life. This review discusses what is known of the active site structures and the mechanisms, together with some recent insights into the evolution of these important enzyme systems.

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Crossref · 10.1039/c3mt00177f

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Authors
Pushie, M. J., Cotelesage, J. J., & George, G. N.
Affiliations
University of Saskatchewan (CA)
Venue
Metallomics
Published
2014
Volume
6
Issue
1
Pages
15-24
ISSN
  • 1756-5901 (ISSN-L)
  • 1756-591X
OpenAlex
W2137509160
Cited by
55
References
100
Citations by year
2026: 2 · 2025: 5 · 2024: 2 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 6 · 2019: 5 · 2018: 7 · 2017: 8 · 2016: 4 · 2015: 4 · 2014: 4
FWCI
0.23
Type
journal article
Language
en
Open access
closed
Locations
  • Metallomics (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Saskatchewan
  • Saskatchewan Health Research Foundation
  • Canadian Institutes of Health Research
  • Natural Sciences and Engineering Research Council of Canada
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
  • Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
Keywords
molybdenum enzymes, tungsten enzymes, molybdopterin, enzyme active site structure, metal oxidation state, enzyme evolution, microbial respiration, nitrogen fixation, human health
MeSH terms
Molybdenum Cofactors, Binding Sites, Coenzymes, Metalloproteins, Models, Molecular, Molybdenum, Oxygen, Pteridines, Transferases, Tungsten, Molecular Structure, Protein Structure, Tertiary, Catalytic Domain

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