A hydrophilic magnetic MOF for the consecutive enrichment of exosomes and exosomal phosphopeptides
Abstract
Hydrophilic Fe3O4@PDA@UIO-66-NH2 is employed to consecutively enrich urinary exosomes and exosomal phosphopeptides through the strong bond between Zr and phosphate groups. The recovery of exosomes reached 95% and 707 phosphopeptides were identified from 255 phosphoproteins via LC-MS/MS. This work will make a great contribution to further functional analysis of exosomes.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Previous studies have demonstrated the existence of electrostatic interactions between exosomes and MOF materials.
“some previous studies have demonstrated the existence of electrostatic interactions between exosomes and MOF materials [56,57].”
Cites
The Chemistry and Applications of Metal-Organic Frameworks
Furukawa, H., Cordova, K. E., O’Keeffe, M., & Yaghi, O. M. (2013) · Science · doi:10.1126/science.1230444 · Cited by 17,033 · see citing works
The biology <b>,</b> function <b>,</b> and biomedical applications of exosomes
Kalluri, R., & LeBleu, V. S. (2020) · Science · doi:10.1126/science.aau6977 · Cited by 10,770 · see citing works
Kowal, J., Arras, G., Colombo, M., Jouve, M., Morath, J. P., Primdal-Bengtson, B., Dingli, F., Loew, D., Tkach, M., & Théry, C. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521230113 · Cited by 3,426 · see citing works
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
Chen, G., Huang, A. C., Zhang, W., Zhang, G., Wu, M., Xu, W., Yu, Z., Yang, J., Wang, B., Sun, H., et al. (2018) · Nature · doi:10.1038/s41586-018-0392-8 · Cited by 2,813 · see citing works
The biology and function of exosomes in cancer
Kalluri, R. (2016) · Journal of Clinical Investigation · doi:10.1172/jci81135 · Cited by 1,849 · see citing works
Ratajczak, J., Miękus, K., Kucia, M., Zhang, J., Reca, R., Dvořák, P., & Ratajczak, M. Z. (2006) · Leukemia · doi:10.1038/sj.leu.2404132 · Cited by 1,630 · see citing works
Extracellular vesicle isolation and characterization: toward clinical application
Xu, R., Greening, D. W., Zhu, H., Takahashi, N., & Simpson, R. J. (2016) · Journal of Clinical Investigation · doi:10.1172/jci81129 · Cited by 879 · see citing works
Merchant, M. L., Rood, I. M., Deegens, J. K., & Klein, J. B. (2017) · Nature Reviews Nephrology · doi:10.1038/nrneph.2017.148 · Cited by 498 · see citing works
Analytical strategies for phosphoproteomics
Thingholm, T. E., Jensen, O. N., & Larsen, M. R. (2009) · PROTEOMICS · doi:10.1002/pmic.200800454 · Cited by 478 · see citing works
Yoon, S., Kovalenko, A., Bogdanov, K., & Wallach, D. (2017) · Immunity · doi:10.1016/j.immuni.2017.06.001 · Cited by 368 · see citing works
Yang, F., Liao, X., Tian, Y. Y., & Li, G. (2017) · Biotechnology Journal · doi:10.1002/biot.201600699 · Cited by 226 · see citing works
Gao, F., Jiao, F., Xia, C., Zhao, Y. C., Ying, W., Xie, Y., Guan, X., Tao, M., Zhang, Y., Qin, W., et al. (2018) · Chemical Science · doi:10.1039/c8sc04197k · Cited by 222 · see citing works
Personalized detection of circling exosomal PD-L1 based on Fe3O4@TiO2 isolation and SERS immunoassay
Pang, Y., Shi, J., Yang, X., Wang, C., Sun, Z., & Xiao, R. (2019) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2019.111800 · Cited by 216 · see citing works
A new UiO-66-NH2 based mixed-matrix membranes with high CO2/CH4 separation performance
Jiang, Y., Liu, C., Caro, J., & Huang, A. (2018) · Microporous and Mesoporous Materials · doi:10.1016/j.micromeso.2018.08.003 · Cited by 212 · see citing works
Doldán, X., Fagúndez, P., Cayota, A., Laíz, J., & Tosar, J. P. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.6b02421 · Cited by 206 · see citing works
Wang, Z. G., Lv, N., Bi, W.-Z., et al. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b01254 · Cited by 158 · see citing works
An aptamer-based new method for competitive fluorescence detection of exosomes
Yu, X., He, L., Pentok, M., Yang, H., Yang, Y., Li, Z., He, N., Deng, Y., Li, S., Liu, T., et al. (2019) · Nanoscale · doi:10.1039/c9nr04050a · Cited by 153 · see citing works
Magneto-Mediated Electrochemical Sensor for Simultaneous Analysis of Breast Cancer Exosomal Proteins
An, Y., Li, R., Zhang, F., & He, P. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.0c00106 · Cited by 150 · see citing works
Zheng, H., Guan, S., Wang, X., Zhao, J., Gao, M., & Zhang, X. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.0c01572 · Cited by 98 · see citing works
Biogenesis of the Posterior Pole Is Mediated by the Exosome/Microvesicle Protein-sorting Pathway
Shen, B., Fang, Y., Wu, N., & Gould, S. J. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.274803 · Cited by 95 · see citing works
The role of extracellular vesicles in phenotypic cancer transformation
Ogorevc, E., Kralj‐Iglič, V., & Veranič, P. (2013) · Radiology and Oncology · doi:10.2478/raon-2013-0037 · Cited by 82 · see citing works
Wang, L., Yang, Y., Liu, Y., Ning, L., Xiang, Y., & Li, G. (2019) · Chemical Communications · doi:10.1039/c9cc00220k · Cited by 79 · see citing works
Nelson, C. A., Szczech, J. R., Dooley, C. J., Xu, Q., Lawrence, M. J., Zhu, H., Jin, S., & Ge, Y. (2010) · Analytical Chemistry · doi:10.1021/ac100877a · Cited by 74 · see citing works
Xie, Y., & Deng, C. (2017) · Scientific Reports · doi:10.1038/s41598-017-01341-y · Cited by 73 · see citing works
Self-citationHou, R., Li, Y., Sui, Z., Yuan, H., Yang, K., Liang, Z., Zhang, L., & Zhang, Y. (2019) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-019-01982-0 · Cited by 56 · see citing works
Zhang, Y., Wu, X.-F., & Tao, W. (2018) · Trends in Analytical Chemistry (TrAC) · doi:10.1016/j.trac.2018.07.014 · Cited by 36 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Kang, Y., Xu, C., Meng, L., Dong, X., Qi, M., & Jiang, D. (2022) · Bioactive materials · doi:10.1016/j.bioactmat.2022.02.012 · Cited by 253 · see citing works
“Furthermore, some previous studies have demonstrated the existence of electrostatic interactions between exosomes and MOF materials [56,57].”
Background
- Authors
- Zhang, N., Sun, N., & Deng, C.
- Affiliations
- Fudan University (CN)
- Corresponding author
- Sun, Nianrong
- Deng, Chunhui
- Venue
- Chemical Communications
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2020
- Volume
- 56
- Issue
- 90
- Pages
- 13999-14002
- ISSN
- 1359-7345 (ISSN-L)
- 1364-548X
- OpenAlex
- W3092356371
- Cited by
- 69
- References
- 26
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 14 · 2023: 16 · 2022: 21 · 2021: 7
- FWCI
- 4.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemical Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Basic Research Program of China (2018YFA0507501)
- National Natural Science Foundation of China (22074019, 22004017, 21425518)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
- Keywords
- magnetic metal-organic frameworks, urinary exosomes, phosphopeptide enrichment, exosome isolation, phosphoproteomics
- MeSH terms
- Metal-Organic Frameworks, Chromatography, Liquid, Humans, Particle Size, Phosphopeptides, Surface Properties, Tandem Mass Spectrometry, Exosomes, Hydrophobic and Hydrophilic Interactions, Magnetic Phenomena, MCF-7 Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review