Cited by 69Advanced Proteomics Techniques and ApplicationsExtracellular vesicles in diseaseAnalytical chemistry methods development

A hydrophilic magnetic MOF for the consecutive enrichment of exosomes and exosomal phosphopeptides

Zhang, Ning, Sun, Nianrong, Deng, Chunhui·2020·Chemical Communications

Abstract

Hydrophilic Fe3O4@PDA@UIO-66-NH2 is employed to consecutively enrich urinary exosomes and exosomal phosphopeptides through the strong bond between Zr and phosphate groups. The recovery of exosomes reached 95% and 707 phosphopeptides were identified from 255 phosphoproteins via LC-MS/MS. This work will make a great contribution to further functional analysis of exosomes.

Integrity signals

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Crossref · 10.1039/d0cc06147f

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26 of 26 references verified0 citation errors · 0 not checkable

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Authors
Zhang, N., Sun, N., & Deng, C.
Affiliations
Fudan University (CN)
Corresponding author
  • Sun, Nianrong
  • Deng, Chunhui
Venue
Chemical Communications
Published
2020
Volume
56
Issue
90
Pages
13999-14002
ISSN
  • 1359-7345 (ISSN-L)
  • 1364-548X
OpenAlex
W3092356371
Cited by
69
References
26
Citations by year
2026: 3 · 2025: 8 · 2024: 14 · 2023: 16 · 2022: 21 · 2021: 7
FWCI
4.20
Type
journal article
Language
en
Open access
closed
Locations
  • Chemical Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Basic Research Program of China (2018YFA0507501)
  • National Natural Science Foundation of China (22074019, 22004017, 21425518)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
Keywords
magnetic metal-organic frameworks, urinary exosomes, phosphopeptide enrichment, exosome isolation, phosphoproteomics
MeSH terms
Metal-Organic Frameworks, Chromatography, Liquid, Humans, Particle Size, Phosphopeptides, Surface Properties, Tandem Mass Spectrometry, Exosomes, Hydrophobic and Hydrophilic Interactions, Magnetic Phenomena, MCF-7 Cells

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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

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