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Cited by 5Advanced Proteomics Techniques and ApplicationsMosquito-borne diseases and controlMass Spectrometry Techniques and Applications

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Re-mining serum proteomics data reveals extensive post-translational modifications upon Zika and dengue infection

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Allgoewer, Kristina, Wu, Shaohuan, Choi, Hyungwon, Vogel, Christine·2023·Molecular Omics
DOI 10.1039/d2mo00258bPMC PMC10175154

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Abstract

Zika virus (ZIKV) and dengue virus (DENV) are two closely related flaviviruses with similar symptoms. However, due to the implications of ZIKV infections for pregnancy outcomes, understanding differences in their molecular impact on the host is of high interest. Viral infections change the host proteome, including post-translational modifications. As modifications are diverse and of low abundance, they typically require additional sample processing which is not feasible for large cohort studies. Therefore, we tested the potential of next-generation proteomics data in its ability to prioritize specific modifications for later analysis. We re-mined published mass spectra from 122 serum samples from ZIKV and DENV patients for the presence of phosphorylated, methylated, oxidized, glycosylated/glycated, sulfated, and carboxylated peptides. We identified 246 modified peptides with significantly differential abundance in ZIKV and DENV patients. Amongst these, methionine-oxidized peptides from apolipoproteins and glycosylated peptides from immunoglobulin proteins were more abundant in ZIKV patient serum and generate hypotheses on the potential roles of the modification in the infection. The results demonstrate how data-independent acquisition techniques can help prioritize future analyses of peptide modifications.

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Metadata

Authors
Allgoewer, K., Wu, S., Choi, H., & Vogel, C.
Affiliations
  • Humboldt-Universität zu Berlin (DE)
  • New York University (US)
  • National University of Singapore (SG)
Corresponding author
Vogel, Christine
ORCID
Venue
Molecular Omics
Publisher
Oxford University Press (OUP)
Published
2023-02-15
Volume
19
Issue
4
Pages
308-320
ISSN
2515-4184 (ISSN-L)
DOI
10.1039/d2mo00258b
PMC
PMC10175154
OpenAlex
W4320925155
Cited by
5
References
1
Citations by year
2026: 1 · 2025: 3 · 2024: 1
FWCI
0.46
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecular Omics (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute of General Medical Sciences (R35GM127089)
  • National Institute of Allergy and Infectious Diseases (75N93019C00052/NH/NIH HHS/United States)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
  • Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Keywords
Zika virus, dengue virus, flavivirus infection, post-translational modification, serum proteomics, mass spectrometry, phosphorylation, methionine oxidation, glycosylation, sulfation, carboxylation, apolipoproteins, immunoglobulins, host response to viral infection
MeSH terms
Zika Virus Infection, Zika Virus, Dengue, Dengue Virus, Humans, Protein Processing, Post-Translational, Proteomics

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