Cited by 13Chemical Synthesis and AnalysisClick Chemistry and ApplicationsBiochemical and Structural Characterization

Rapid flow-based synthesis of post-translationally modified peptides and proteins: a case study on MYC's transactivation domain

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Williams, Elyse T., Schiefelbein, Kevin, Schuster, Matthias, Ahmed, Ikhlas M. M., De Vries, Marije, Beveridge, Rebecca, Zerbe, Oliver, Hartrampf, Nina·2024·Chemical Science

Published version of a preprint

Abstract

Automated flow peptide synthesis (AFPS) gives access to peptides and proteins with post-translational modifications (PTMs) and enables investigation of PTM-mediated regulation, and was used to study the PTM-dependence of the MYC:Bin1 interaction.

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Authors
Williams, E. T., Schiefelbein, K., Schuster, M., Ahmed, I. M. M., De Vries, M., Beveridge, R., Zerbe, O., & Hartrampf, N.
Affiliations
  • University of Zurich (CH)
  • University of Strathclyde (GB)
  • Department for Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral St, Glasgow G1 1XL, UK
Corresponding author
Hartrampf, Nina
Venue
Chemical Science
Published
2024
Volume
15
Issue
23
Pages
8756-8765
ISSN
  • 2041-6520 (ISSN-L)
  • 2041-6539
OpenAlex
W4396698955
Cited by
13
References
55
Citations by year
2026: 6 · 2025: 5 · 2024: 2
FWCI
1.77
Type
journal article
Language
en
Open access
Locations
  • Chemical Science (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Zurich Open Repository and Archive (University of Zurich) (submittedVersion, cc-by)
Funders
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (project grant no. 200021_200865 (N. H.))
  • University of Strathclyde (Chancellor's Fellowship (R. B.))
  • Universität Zürich (UZH PostDoc grant (E. T. W.))
  • Dr. Helmut Legerlotz-Stiftung ((N. H.))
  • UK Research and Innovation (MR/T020970/1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chemical Synthesis and Analysis
  • Physical Sciences › Chemistry › Organic Chemistry › Click Chemistry and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
post-translational modification, peptide synthesis, BIN1, protein-protein interaction, phosphorylation, glycosylation, methylation, sulfation, acetylation, heteronuclear NMR, native mass spectrometry, protein analogues

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