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Cited by 273

The DOI matches its OpenAlex record; 27 of the 27 references it cites were found in a registry or an index.

Characterization of the high-affinity monocarboxylate transporter MCT2 in Xenopus laevis oocytes

Open access
Stefan Bröer, Angelika Bröer, Hans-Peter SCHNEIDER, Carola F. Stegen, Andrew P. Halestrap, Joachim W. Deitmer·1999·Biochemical Journal
DOI 10.1042/0264-6021:3410529
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Abstract

Observations on lactate transport in brain cells and cardiac myocytes indicate the presence of a high-affinity monocarboxylate transporter. The rat monocarboxylate transporter isoform MCT2 was analysed by expression in Xenopus laevis oocytes and the results were compared with the known characteristics of lactate transport in heart and brain. Monocarboxylate transport via MCT2 was driven by the H(+) gradient over the plasma membrane. Uptake of lactate strongly increased with decreasing pH, showing half-maximal stimulation at pH 7.2. A wide variety of monocarboxylates and ketone bodies, including lactate, pyruvate, beta-hydroxybutyrate, acetoacetate, 2-oxoisovalerate and 2-oxoisohexanoate, were substrates of MCT2. All substrates had a high affinity for MCT2. For lactate a K(m) value of 0.74+/-0.07 mM was determined at pH 7.0. For the other substrates, K(i) values between 100 microM and 1 mM were measured for inhibition of lactate transport, which is about one-tenth of the corresponding values for the ubiquitously expressed monocarboxylate transporter isoform MCT1. Monocarboxylate transport via MCT2 could be inhibited by alpha-cyano-4-hydroxycinnamate, anion-channel inhibitors and flavonoids. It is suggested that cells which express MCT2 preferentially use lactate and ketone bodies as energy sources.

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Metadata

Authors
Bröer, S., Bröer, A., SCHNEIDER, H.-P., Stegen, C. F., Halestrap, A. P., & Deitmer, J. W.
Venue
Biochemical Journal
Publisher
Portland Press
Published
1999-08-01
Volume
341
Issue
3
Pages
529-529
ISSN
0264-6021 (ISSN-L)
DOI
10.1042/0264-6021:3410529
OpenAlex
W2031004694
Cited by
273
References
27
FWCI
4.08
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Characterization of the high-affinity monocarboxylate transporter MCT2 in Xenopus laevis oocytes. https://app.cochat.ai/papers/10.1042/0264-6021:3410529
The paper
Bröer, S., Bröer, A., SCHNEIDER, H.-P., Stegen, C. F., Halestrap, A. P., & Deitmer, J. W. (1999). Characterization of the high-affinity monocarboxylate transporter MCT2 in Xenopus laevis oocytes. Biochemical Journal. https://doi.org/10.1042/0264-6021%3A3410529

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.