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Electron-spin-resonance evidence for enzymic reduction of oxygen to a free radical, the superoxide ion
Open accessAbstract
1. An electron-spin-resonance signal with g∥2·08 and g⊥2·00 is observed by the rapid-freezing technique during the oxidation of substrates by molecular oxygen catalysed by xanthine oxidase at pH10. 2. The intensity of this signal is shown to depend on oxygen rather than on enzyme concentration, indicating that it is due to an oxygen free radical and not to the enzyme. 3. The same species is shown to be produced in the reaction at pH10 between hydrogen peroxide and periodate ions. Studies with this system have facilitated comparison of the properties of the oxygen radical with data in the literature on the products of pulse radiolysis of oxygenated water over a wide pH range. 4. It is concluded that the species observed is the superoxide ion, O2−, and that the stability of this ion is greatly increased in alkaline solution. A mechanism explaining the alkaline stability is proposed. 5. The importance of O2− in the enzymic reaction is discussed.
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Citations
Cited by
McCord, J. M., & Fridovich, I. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)63504-5 · Cited by 12,952
Superoxide radical and superoxide dismutases
Fridovich, I. (1995) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.64.070195.000525 · Cited by 3,455
Quantitative Aspects of the Production of Superoxide Anion Radical by Milk Xanthine Oxidase
Fridovich, I. (1970) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)62884-4 · Cited by 1,035
Nishino, T., Okamoto, K., Eger, B. T., Pai, E. F., & Nishino, T. (2008) · The FEBS Journal · doi:10.1111/j.1742-4658.2008.06489.x · Cited by 384
Weibel, M. K., & Bright, H. J. (1971) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)62246-x · Cited by 349
Metadata
- Authors
- Knowles, P. F., Gibson, J. F., Pick, F. M., & Bray, R. C.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1969-01-01
- Volume
- 111
- Issue
- 1
- Pages
- 53-58
- ISSN
- 0306-3283 (ISSN-L)
- OpenAlex
- W2270686920
- Cited by
- 244
- References
- 0
- FWCI
- 20.45
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Knowles, P. F., Gibson, J. F., Pick, F. M., & Bray, R. C. (1969). Electron-spin-resonance evidence for enzymic reduction of oxygen to a free radical, the superoxide ion. Biochemical Journal. https://doi.org/10.1042/bj1110053
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
