The metabolism of lipids in mouse pancreatic islets. The oxidation of fatty acids and ketone bodies
Open accessAbstract
The rate of oxidation of 14C-labelled fatty acids and of ketone bodies was measured in isolated pancreatic islets of obese-hyperglycaemic mice (ob/ob). The following main observations were made. 1. Octanoate, palmitate and oleate were all converted into CO2 by the pancreatic islets. Octanoate was oxidized with the highest rate followed by palmitate and oleate. 2. The rate of oxidation of 0.7 mM-palmitate was 3.1 pmol/h per mug drug weight. This was decreased by 50% in the presence of 16.7 mM-glucose. The rate of palmitate oxidation was also inhibited by 2-bromostearate. The palmitate oxidation showed a concentration-dependent increase, which was most marked between 0.25 and 1.0 mM. 3. Octanoate (5 mM) had no effect on the rate of oxidation of 3.3 mM- glucose. 4. Acetoacetate (5 mM) and D-3-hydroxybutyrate (5 mM) were oxidized at rates of 5.9 and 5.4 pmol/h per mug dry weight respectively. These rates were less than 10% of those found in kidney-cortex slices. The magnitude of the oxidation rates found for fatty acids and for ketone bodies suggest that these substrates represent important metabolic fuels for the pancreatic B-cells.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The rate of oxidation of 0.7 mM oleate and palmitate is comparable to that of 3 mM glucose.
“Thus, the rate of oxidation of 0.7 mM oleate and palmitate is comparable to that of 3 mM glucose (63).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [63]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Abnormalities in carbohydrate tolerance associated with elevated plasma nonesterified fatty acids.
Schalch, D. S., & Kipnis, D. M. (1965) · Journal of Clinical Investigation · doi:10.1172/jci105308 · Cited by 210 · see citing works
Flagged first, then by citations
Research works printed but not linked: 3
- Berne, C. (1975a) Enzyme in the press Berne, C. (1975b) Biochem. J. 152, 667-673
- Scheig, R. (1968) in Medium Chain Triglycerides (Senior, J. R., ed.), pp. 39-49, University of Pennsylvania Press, Philadelphia
- Spector, A. A. (1971) in Biochemistry andPharmacology of Free Fatty Acids (Holmes, W. L. & Bortz, W. M., eds.), pp. 130-176, S. Karger, Basel Spector, A. A. & Steinberg, D. (1965) J. Biol. Chem. 240, 3747-3753
Cited by
Sorted by citations
Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
Zhou, Y. P., & Grill, V. E. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117042 · Cited by 677 · see citing works
The New Biology and Pharmacology of Glucagon
Müller, T. D., Finan, B., Clemmensen, C., DiMarchi, R. D., & Tschöp, M. H. (2017) · Physiological Reviews · doi:10.1152/physrev.00025.2016 · Cited by 419 · see citing works
Stein, D. T., Stevenson, B. E., Chester, M. W., Basit, M., Daniels, M. B., Turley, S. D., & McGarry, J. D. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119546 · Cited by 352 · see citing works
Elsner, M., Gehrmann, W., & Lenzen, S. (2011) · Diabetes · doi:10.2337/db09-1401 · Cited by 221 · see citing works
Paolisso, G., Gambardella, A., Amato, L., Tortoriello, R., D'Amore, A., Varricchio, M., & D'Onofrio, F. (1995) · Diabetologia · doi:10.1007/bf00401761 · Cited by 217 · see citing works
Pinnick, K., Neville, M., Clark, A., & Fielding, B. (2010) · Journal of Cellular Biochemistry · doi:10.1002/jcb.22445 · Cited by 55 · see citing works
- Authors
- Berne, C.
- Affiliations
- Uppsala University (SE)
- Corresponding author
- Berne, C
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1975-12-01
- Volume
- 152
- Issue
- 3
- Pages
- 661-666
- ISSN
- 0264-6021 (ISSN-L)
- PMC
- PMC1172521
- OpenAlex
- W1500111450
- Cited by
- 77
- References
- 1
- Citations by year
- 2024: 1 · 2023: 1 · 2021: 1 · 2020: 2 · 2019: 3 · 2017: 3 · 2016: 2 · 2014: 2
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Biochemical Acid Research Studies
- Keywords
- pancreatic islets, fatty acid oxidation, ketone oxidation, pancreatic beta cells, palmitate, octanoate, oleate, acetoacetate, beta-hydroxybutyrate, glucose inhibition, islet metabolism, ob/ob mice, obesity
- MeSH terms
- Animals, Caprylates, Fatty Acids, Female, Glucose, Islets of Langerhans, Ketone Bodies, Kidney Cortex, Male, Mice, Obese, Oleic Acids, Oxidation-Reduction, Palmitates, Stearates, Time Factors, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
