Stimulation of hepatic lipogenesis and acetyl-coenzyme A carboxylase by vasopressin
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The effect of vasopressin on the short-term regulation of fatty acid synthesis was studied in isolated hepatocytes from rats fed ad libitum. Vasopressin stimulates fatty acid synthesis by 30-110%. This increase is comparable with that obtained with insulin. Angiotensin also stimulates fatty acid synthesis, whereas phenylephrine does not. The dose-response curve for vasopressin-stimulated lipogenesis is similar to the dose-response curve for glycogenolysis and release of lactate plus pyruvate. Vasopression also stimulates acetyl-CoA carboxylase activity in a dose-dependent manner. Vasopressin does not relieve glucagon-inhibited lipogenesis, whereas insulin does. The action of vasopressin on hepatic lipogenesis is decreased, but not suppressed, in Ca2+-depleted hepatocytes. The results suggest that vasopressin acts on lipogenesis by increasing availability of lipogenic substrate (lactate + pyruvate) and by activating acetyl-CoA carboxylase.
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Assimacopoulos-Jeannet and colleagues found that VP stimulated fat synthesis in isolated hepatocytes, but this effect disappeared when lactate in the medium was raised, which itself increased baseline lipogenesis.
“Assimacopoulos-Jeannet et al [48] showed that VP increased lipogenesis in isolated hepatocytes, but when concentration of lactate in the medium was increased, basal lipogenesis was increased and the stimulatory effect of VP was lost.”
From Vasopressin and hydration play a major role in the development of glucose intolerance and hepatic steatosis in obese rats (2015) · cites this paper as [48]
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- Authors
- Assimacopoulos-Jeannet, F., Denton, R. M., & Jeanrenaud, B.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1981-09-15
- Volume
- 198
- Issue
- 3
- Pages
- 485-490
- ISSN
- 0264-6021 (ISSN-L)
- PMC
- PMC1163292
- OpenAlex
- W2397103699
- Cited by
- 29
- References
- 37
- Citations by year
- 2024: 1 · 2015: 1
- FWCI
- 2.58
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- vasopressin, hepatic lipogenesis, fatty acid synthesis, acetyl-CoA carboxylase, hepatocytes, insulin, angiotensin, phenylephrine, glucagon, lactate, pyruvate, calcium depletion
- MeSH terms
- Acetyl-CoA Carboxylase, Angiotensin II, Animals, Arginine Vasopressin, Dose-Response Relationship, Drug, Glucagon, Insulin, Ligases, Lipids, Liver, Male, Rats, Inbred Strains, Stimulation, Chemical, Rats, In Vitro Techniques
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