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Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of ‘free’ iron in biological systems by using bleomycin-dependent degradation of DNA
Open accessAbstract
Bleomycin in the presence of iron(II) degrades DNA to form a thiobarbituric acid-reactive product. This has been made the basis of a specific assay method for ‘free’ iron in biological fluids. Human synovial fluid, human cerebrospinal fluid and rat pleural-exudate fluid were found to contain micromolar concentrations of ‘free’ iron, which would be sufficient to allow formation of the hydroxyl radical from superoxide and hydrogen peroxide generated in vivo. This assay method does not detect iron bound to transport proteins or to enzymes.
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Citations
Cited by
Oxygen toxicity, oxygen radicals, transition metals and disease
Halliwell, B., & Gutteridge, J. M. C. (1984) · Biochemical Journal · doi:10.1042/bj2190001 · Cited by 5,330
Self-citation[1] Role of free radicals and catalytic metal ions in human disease: An overview
Halliwell, B., & Gutteridge, J. M. C. (1990) · Methods in Enzymology · doi:10.1016/0076-6879(90)86093-b · Cited by 4,994
Self-citationAntioxidants in health and disease
Young, I. S., & Woodside, J. V. (2001) · Journal of Clinical Pathology · doi:10.1136/jcp.54.3.176 · Cited by 1,949
The importance of free radicals and catalytic metal ions in human diseases.
Halliwell, B., & Gutteridge, J. M. C. (1985) · Molecular Aspects of Medicine · doi:10.1016/0098-2997(85)90001-9 · Cited by 941
Self-citationNon-transferrin bound iron: a key role in iron overload and iron toxicity
Brissot, P., Ropert, M., Le Lan, C., & Loréal, O. (2012) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2011.07.014 · Cited by 654
Metadata
- Authors
- Gutteridge, J. M. C., Rowley, D. A., & Halliwell, B.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1981-10-01
- Volume
- 199
- Issue
- 1
- Pages
- 263-265
- ISSN
- 0264-6021 (ISSN-L)
- OpenAlex
- W125730286
- Cited by
- 467
- References
- 0
- FWCI
- 10.73
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of ‘free’ iron in biological systems by using bleomycin-dependent degradation of DNA. https://app.cochat.ai/papers/10.1042/bj1990263
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- Gutteridge, J. M. C., Rowley, D. A., & Halliwell, B. (1981). Superoxide-dependent formation of hydroxyl radicals in the presence of iron salts. Detection of ‘free’ iron in biological systems by using bleomycin-dependent degradation of DNA. Biochemical Journal. https://doi.org/10.1042/bj1990263
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
