Cited by 118Sphingolipid Metabolism and SignalingLipid metabolism and biosynthesisPlant biochemistry and biosynthesis

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DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine

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OMAE, Fumio, MIYAZAKI, Masao, ENOMOTO, Ayako, SUZUKI, Minoru, SUZUKI, Yusuke, SUZUKI, Akemi·2004·Biochemical Journal

DOI 10.1042/bj20031425PMC PMC1224108

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Abstract

The C-4 hydroxylation of sphinganine and dihydroceramide is a rate-limiting reaction in the biosynthesis of phytosphingolipids. Mouse DES1 (MDES1) cDNA homologous to the Drosophila melanogaster degenerative spermatocyte gene-1 (des-1) cDNA leads to sphingosine Δ4-desaturase activity, and another mouse homologue, MDES2, has bifunctional activity, producing C-4 hydroxysphinganine and Δ4-sphingenine in yeast [Ternes, Franke, Zahringer, Sperling and Heinz (2002) J. Biol. Chem. 277, 25512–25518]. Here, we report the characterization of mouse DES2 (MDES2) using an in vitro assay with a homogenate of COS-7 cells transfected with MDES2 cDNA and N-octanoyl-sphinganine and sphinganine as substrates. MDES2 protein prefers dihydroceramide as a substrate to sphinganine, and exhibits dihydroceramide Δ4-desaturase and C-4 hydroxylase activities. MDES2 mRNA content was high in the small intestine and abundant in the kidney. In situ hybridization detected signals of MDES2 mRNA in the crypt cells. Immunohistochemistry using an anti-MDES2 peptide antibody stained the crypt cells and the adjacent epithelial cells. These results suggest that MDES2 is the dihydroceramide C-4 hydroxylase responsible for the biosynthesis of enriched phytosphingoglycolipids in the microvillous membranes of intestinal epithelial cells.

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Authors: OMAE, F., MIYAZAKI, M., ENOMOTO, A., SUZUKI, M., SUZUKI, Y., & SUZUKI, A.
Affiliations: RIKEN (JP)
ORCID: MIYAZAKI, Masao
SUZUKI, Minoru
SUZUKI, Yusuke
Venue: Biochemical Journal
Publisher: Portland Press Ltd.
Published: 2004-05-01
Volume: 379
Issue: 3
Pages: 687-695
ISSN: 0264-6021 (ISSN-L)
1470-8728
DOI: 10.1042/bj20031425
PMC: PMC1224108
OpenAlex: W2103244006
Cited by: 118
References: 44
Citations by year: 2026: 2 · 2025: 4 · 2024: 4 · 2023: 11 · 2022: 8 · 2021: 6 · 2020: 4 · 2019: 2 · 2018: 5 · 2017: 2 · 2016: 7 · 2015: 6 · 2014: 6 · 2013: 6 · 2012: 3
FWCI: 1.78
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Biochemical Journal (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Tokyo Medical and Dental University
RIKEN
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords: small intestine, intestinal epithelial cells, crypt cells, sphinganine, dihydroceramide
MeSH terms: Animals, Cell Extracts, Ceramides, Mixed Function Oxygenases, Immunohistochemistry, Intestine, Small, Membrane Proteins, Multienzyme Complexes, Organ Specificity, Oxidoreductases, RNA, Messenger, Sphingosine, Transfection, In Situ Hybridization, COS Cells, Fatty Acid Desaturases, Mice

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DES2 protein is responsible for phytoceramide biosynthesis in the mouse small intestine

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