The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Production of hydroxyl radicals from the simultaneous generation of superoxide and nitric oxide
Open accessAbstract
Both nitric oxide (NO) and superoxide are generated by macrophages, neutrophils and endothelial cells. It has been postulated that the generation of these two radicals under physiological conditions can lead to the formation of peroxynitrite and (as a result of the homolytic lysis of this molecule) the production of hydroxyl radicals. We have used 3-morpholinosydnonimine N-ethylcarbamide (SIN-1), a sydnonimine capable of generating both NO and superoxide simultaneously, to test this hypothesis. SIN-1 (1 mM) generated superoxide and NO at rates of 7.02 microM/min and 3.68 microM/min respectively in phosphate-buffered saline, pH 7.2, at 37 degrees C. Incubation of SIN-1 with both deoxyribose and sodium benzoate resulted in the formation of malondialdehyde (MDA). In addition, the incubation of SIN-1 with sodium benzoate resulted in the production of compounds with fluorescence emission spectra characteristic of hydroxylated products. Both the production of MDA and the generation of fluorescent compounds were inhibited by the hydroxyl radical scavenger mannitol. In all the above respects, SIN-1 mimicked the production of hydroxyl radicals from the ascorbate-driven Fenton reaction. Catalase had no effect on the SIN-1-dependent generation of MDA, and superoxide dismutase was partially inhibitory. SIN-1 produces an oxidant with the properties of the hydroxyl radical by a mechanism clearly different to that of the Fenton reaction. We conclude that the simultaneous production of NO and superoxide from SIN-1 results in the formation of hydroxyl radicals.
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Cites
Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor
Palmer, R. M. J., Ferrige, A. G., & Moncada, S. (1987) · Nature · doi:10.1038/327524a0 · Cited by 10,704
Self-citationBeckman, J. S., Beckman, T. W., Chen, J., Marshall, P. A., & Freeman, B. A. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.4.1620 · Cited by 7,029
[1] Role of free radicals and catalytic metal ions in human disease: An overview
Halliwell, B., & Gutteridge, J. M. C. (1990) · Methods in Enzymology · doi:10.1016/0076-6879(90)86093-b · Cited by 4,994
Free Radicals in Biology and Medicine
Land, E. T. (1990) · International Journal of Radiation Biology · doi:10.1080/09553009014552071 · Cited by 4,854
Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor
Gryglewski, R. J., Palmer, R. M. J., & Moncada, S. (1986) · Nature · doi:10.1038/320454a0 · Cited by 2,400
Self-citationHalliwell, B., Gutteridge, J. M. C., & Aruoma, O. I. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90222-3 · Cited by 2,362
Oxygen radicals and human disease
CROSS, C. E., HALLIWELL, B., BORISH, E. T., PRYOR, W. A., AMES, B. N., SAUL, R. L., McCORD, J. M., & HARMAN, D. (1987) · Annals of Internal Medicine · doi:10.7326/0003-4819-107-4-526 · Cited by 1,856
Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate
Marletta, M. A., Yoon, P. S., Iyengar, R., Leaf, C. D., & Wishnok, J. S. (1988) · Biochemistry · doi:10.1021/bi00424a003 · Cited by 1,600
The Role of Endothelium in the Responses of Vascular Smooth Muscle to Drugs
Furchgott, R. F. (1984) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.24.040184.001135 · Cited by 950
Feelisch, M., & Noack, E. A. (1987) · European Journal of Pharmacology · doi:10.1016/0014-2999(87)90493-6 · Cited by 888
Cited by
Nitric Oxide and Peroxynitrite in Health and Disease
Pacher, P., Beckman, J. S., & Liaudet, L. (2007) · Physiological Reviews · doi:10.1152/physrev.00029.2006 · Cited by 6,390
Lipton, S. A., Choi, Y.-B., Pan, Z.-H., Lei, S. Z., Chen, H.-S. V., Sucher, N. J., Loscalzo, J., Singel, D. J., & Stamler, J. S. (1993) · Nature · doi:10.1038/364626a0 · Cited by 2,433
Wang, H., & Joseph, J. A. (1999) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(99)00107-0 · Cited by 2,346
Nitric oxide donors: chemical activities and biological applications
Wang, P. G., Xian, M., Tang, X., Wu, X., Wen, Z., Cai, T., & Janczuk, A. J. (2002) · Chemical Reviews · doi:10.1021/cr000040l · Cited by 1,337
Duncan, C., Dougall, H., Johnston, P., Green, S., Brogan, R., Leifert, C., Smith, L., Golden, M., & Benjamin, N. (1995) · Nature Medicine · doi:10.1038/nm0695-546 · Cited by 734
Metadata
- Authors
- Hogg, N., Darley-Usmar, V. M., Wilson, M. T., & Moncada, S.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1992-01-15
- Volume
- 281
- Issue
- 2
- Pages
- 419-424
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W1923609106
- Cited by
- 698
- References
- 20
- FWCI
- 26.81
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Hogg, N., Darley-Usmar, V. M., Wilson, M. T., & Moncada, S. (1992). Production of hydroxyl radicals from the simultaneous generation of superoxide and nitric oxide. Biochemical Journal. https://doi.org/10.1042/bj2810419
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