The respiratory-chain poison antimycin A promotes the formation of DNA single-strand breaks and reduces toxicity in U937 cells exposed to t-butylhydroperoxide
Open accessAbstract
Antimycin A at levels that abolish oxygen consumption had a slight, although statistically significant, inhibitory effect on the toxicity elicited by t-butylhydroperoxide in U937 cells. The protective effect was observed after 6 h of post-treatment incubation, but was no longer apparent after 24 h. Unexpectedly, these events were associated with a marked accumulation of DNA single-strand breaks produced by low levels of t-butylhydroperoxide. Both an oxygen- and a carbon-centred radical were found to arise during treatment with t-butylhydroperoxide, and their formation was significantly lowered by antimycin A. Thus inhibition of electron transport at the level of complex III appears (a) to decrease the formation of toxic species which mediate, at least partially, the lethal effects elicited by t-butylhydroperoxide, and (b) to enhance the formation of DNA-damaging species generated at low concentrations of t-butylhydroperoxide. Rotenone and cyanide, which respectively inhibit complexes I and IV, did not affect DNA damage elicited by t-butylhydroperoxide. These results suggest that DNA single-strand breaks do not mediate the toxicity of t-butylhydroperoxide, and that specific mitochondrial functions might modulate the formation of toxic and of DNA-damaging species generated by organic hydroperoxides.
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Cites
Hydroperoxide metabolism in mammalian organs.
Chance, B., Sies, H., & Boveris, A. (1979) · Physiological Reviews · doi:10.1152/physrev.1979.59.3.527 · Cited by 5,827 · see citing works
Schulze‐Osthoff, K., Bakker, A. C., Vanhaesebroeck, B., Beyaert, R., Jacob, W. A., & Fiers, W. C. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42768-8 · Cited by 935 · see citing works
DNA repair: a laboratory manual of research procedures
(1981) · Annales de l Institut Pasteur Virologie · doi:10.1016/s0769-2617(81)80032-9 · Cited by 815 · see citing works
Robinson, J., & Cooper, J. M. (1970) · Analytical Biochemistry · doi:10.1016/0003-2697(70)90310-6 · Cited by 381 · see citing works
O’Donnell, V. B., Spycher, S. E., & Azzi, A. (1995) · Biochemical Journal · doi:10.1042/bj3100133 · Cited by 156 · see citing works
Davies, M. J. (1989) · Biochemical Journal · doi:10.1042/bj2570603 · Cited by 131 · see citing works
Mechanism of Radical Production from the Reaction of Cytochrome c with Organic Hydroperoxides.
Barr, D. P., & Mason, R. P. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.21.12709 · Cited by 129 · see citing works
Cantoni, O., Murray, D. L., & Meyn, R. E. (1986) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/0167-4781(86)90073-4 · Cited by 102 · see citing works
Self-citationDetection of oxygen-derived radicals in biological systems using electron spin resonance.
Mason, R. P., Hanna, P. M., Burkitt, M. J., & Kadiiska, M. B. (1994) · Environmental Health Perspectives · doi:10.1289/ehp.94102s1033 · Cited by 86 · see citing works
Elaboration of cellular DNA breaks by hydroperoxides
Baker, M. A., & He, S. (1991) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(91)90137-r · Cited by 81 · see citing works
Coleman, J. B., Gilfor, D., & Farber, J. L. (1989) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)09105-9 · Cited by 74 · see citing works
O’Donnell, V. B., & Burkitt, M. J. (1994) · Biochemical Journal · doi:10.1042/bj3040707 · Cited by 47 · see citing works
Sandström, B. E. R. (1991) · Free Radical Research Communications · doi:10.3109/10715769109049128 · Cited by 40 · see citing works
Thiol depletion induces lethal cell injury in cultured cardiomyocytes
Dhanbhoora, C. M., & Babson, J. R. (1992) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(92)90375-7 · Cited by 31 · see citing works
Guidarelli, A., Sestili, P., Cossarizza, A., et al. (1995) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)12220-9 · Cited by 27 · see citing works
Self-citationDileepan, K. N., & Kennedy, J. (1985) · Biochemical Journal · doi:10.1042/bj2250189 · Cited by 17 · see citing works
Sestili, P., Cattabeni, F., & Cantoni, O. (1994) · Biochemical Pharmacology · doi:10.1016/0006-2952(94)90454-5 · Cited by 10 · see citing works
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- Authors
- GUIDARELLI, A., BRAMBILLA, L., ROTA, C., TOMASI, A., CATTABENI, F., & CANTONI, O.
- Affiliations
- University of Urbino (IT)
- University of Modena and Reggio Emilia (IT)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1996-07-15
- Volume
- 317
- Issue
- 2
- Pages
- 371-375
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1217497
- OpenAlex
- W272280634
- Cited by
- 28
- References
- 17
- Citations by year
- 2024: 1 · 2021: 1 · 2020: 1
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
- Keywords
- antimycin A, tert-butyl hydroperoxide, single-strand breaks, U937 cells, oxidative DNA damage, organic hydroperoxides, oxygen-centred radicals, carbon-centered radicals, rotenone, cyanide, mitochondrial function, cytotoxicity
- MeSH terms
- Antimycin A, Cells, Cultured, DNA Damage, DNA Repair, Dose-Response Relationship, Drug, Electron Spin Resonance Spectroscopy, Electron Transport, Enzyme Inhibitors, Humans, Mitochondria, Peroxides, Reactive Oxygen Species, Toxicity Tests, tert-Butylhydroperoxide
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