Insulin-like growth factor I and insulin induce adipogenic-related gene expression in fetal brown adipocyte primary cultures
Open accessAbstract
Fetal rat brown adipocytes show high-affinity binding sites for both insulin-like growth factor I (IGF-I) and insulin. Cell culture for 24 h in the presence of IGF-I or insulin, independently, up-regulated the mRNA expression of adipogenic-related genes, such as fatty acid synthase (FAS), glycerol-3-phosphate dehydrogenase and insulin-regulated glucose transporter Glut4, and down-regulated the expression of phosphoenolpyruvate carboxykinase mRNA in a dose-dependent manner. Moreover, both IGF-I and insulin increased the FAS gene transcription rate at 2 h, producing a time-dependent accumulation of FAS mRNA. Furthermore IGF-I or insulin increased glucose uptake and lipid content throughout the 24 h culture period. Our results suggest that both IGF-I and insulin are major signals involved in initiating and/or maintaining the expression of adipogenic-related genes in fetal rat brown adipocytes.
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Increased adiposity despite exposure to maternal nutrient restriction is accompanied by an increased abundance of IGF receptors, suggesting enhanced adipose tissue sensitivity to the anabolic effects of IGFs.
“Increased adiposity despite exposure to maternal nutrient restriction was accompanied by an increased abundance of IGF receptors, suggesting enhanced adipose tissue sensitivity to the anabolic effects of IGFs [67].”
From Developmental programming of offspring adipose tissue biology and obesity risk (2021) · cites this paper as [67]
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Developmental programming of offspring adipose tissue biology and obesity risk
Rodgers, A., & Sferruzzi-Perri, A. N. (2021) · International Journal of Obesity · doi:10.1038/s41366-021-00790-w · Cited by 57 · see citing works
Micke, G. C., Sullivan, T. M., McMillen, I. C., Gentili, S., & Perry, V. E. A. (2011) · Reproduction · doi:10.1530/rep-10-0332 · Cited by 34 · see citing works
- Authors
- TERUEL, T., VALVERDE, A. M., BENITO, M., & LORENZO, M.
- Affiliations
- Universidad Complutense de Madrid (ES)
- Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Universidad Complutense, 28040-Madrid, Spain
- ORCID
- BENITO, Manuel
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1996-10-15
- Volume
- 319
- Issue
- 2
- Pages
- 627-632
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1217813
- OpenAlex
- W2336091041
- Cited by
- 78
- References
- 0
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 1 · 2019: 1 · 2018: 1 · 2015: 1 · 2014: 2 · 2013: 5 · 2012: 1
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- insulin-like growth factor I, insulin, brown adipocytes, adipogenesis, gene expression regulation, fatty acid synthase, glycerol-3-phosphate dehydrogenase, GLUT4, phosphoenolpyruvate carboxykinase, glucose uptake, lipid accumulation
- MeSH terms
- Animals, Adipose Tissue, Brown, Cells, Cultured, Female, Gene Expression Regulation, Glycerolphosphate Dehydrogenase, Insulin, Insulin-Like Growth Factor I, Monosaccharide Transport Proteins, Muscle Proteins, Phosphoenolpyruvate Carboxykinase (GTP), Rats, Wistar, Glucose Transporter Type 4, Rats, Fatty Acid Synthases
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