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Cited by 118Selenium in Biological SystemsWound Healing and TreatmentsRedox biology and oxidative stress

The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.

A novel type of glutathione peroxidase: expression and regulation during wound repair

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MUNZ, Barbara, FRANK, Stefan, HÜBNER, Griseldis, OLSEN, Eydfinnur, WERNER, Sabine·1997·Biochemical Journal
DOI 10.1042/bj3260579PMC PMC1218708
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Abstract

We have previously identified and cloned a novel keratinocyte growth factor (KGF)-regulated gene in human keratinocytes that encodes the human homologue of a bovine non-selenium glutathione peroxidase (GPx). To gain insight into the regulation of this gene in vivo, we isolated the murine homologue from a mouse skin cDNA library. In vitro transcription/translation demonstrated that the cDNA encodes a 27 kDa protein. Furthermore, we amplified by PCR a partial cDNA that most likely corresponds to a related gene. RNase protection analysis revealed tissue-specific expression of both genes and the occurrence of alternative splicing or RNA editing of at least one of the primary transcripts. Similar to that of KGF, expression of GPx was strongly induced after cutaneous injury, and each isoform displayed unique kinetics of expression during the repair process. In situ hybridization studies demonstrated high levels of GPx mRNA in keratinocytes of the hyperproliferative epithelium at the wound edge. Since these cells express functional KGF receptors, induction of GPx expression by KGF might also occur in vivo. These data suggest a role for GPx in the protection of epithelial cells against oxidative stress, particularly during the inflammatory phase of wound repair.

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  1. Rubin, J. S., Osada, H., Finch, P. W., Taylor, W. G., Rudikoff, S. and Aaronson, S. A. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 802-806 Received 13 March 1997/6 May 1997 ; accepted 9 May 1997
  2. Werner, S., Peters, K. G., Longaker, M. T., Fuller-Pace, F., Banda, M. J. and Williams, L. T. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 6896-6900
  3. Miki, T., Bottaro, D. P., Fleming, T. P., Smith, C. L., Burgess, W. H., Chan, A. M. L. and Aaronson, S. A. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 246-250
  4. Aviv, H. and Leder, P. (1972) Proc. Natl. Acad. Sci. U.S.A. 69, 6772-6774
  5. Ames, B. N. and Shigenaga, M. K. (1993) DNA and Free Radicals (Halliwell, B. and Aruoma, O. I., eds.), pp. 1-15, Ellis Harwood Publishers, Amsterdam 32

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Metadata

Authors
MUNZ, B., FRANK, S., HÜBNER, G., OLSEN, E., & WERNER, S.
Affiliations
  • Max Planck Institute of Biochemistry (DE)
  • Danish Centre for Human Genome Research, Ole Worms Alle 170, DK-8000 Aarhus C, Denmark
Corresponding author
  • MUNZ, Barbara
  • FRANK, Stefan
  • WERNER, Sabine
ORCID
WERNER, Sabine
Venue
Biochemical Journal
Publisher
Portland Press Ltd.
Published
1997-09-01
Volume
326
Issue
2
Pages
579-585
ISSN
  • 0264-6021 (ISSN-L)
  • 1470-8728
DOI
10.1042/bj3260579
PMC
PMC1218708
OpenAlex
W2267571706
Cited by
118
References
32
Citations by year
2026: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 5 · 2013: 4 · 2012: 1
FWCI
10.59
Type
journal article
Language
en
Open access
Locations
  • Biochemical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Eli Lilly and Company
  • Verband der Chemischen Industrie
  • Deutsche Forschungsgemeinschaft
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
  • Health Sciences › Medicine › Surgery › Wound Healing and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
glutathione peroxidase, keratinocyte growth factor, keratinocytes, wound healing, skin wounds, gene expression regulation, alternative splicing, RNA editing, tissue-specific gene expression, oxidative stress, inflammatory phase, mouse skin
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Cattle, Cell Division, Cloning, Molecular, Epithelial Cells, Epithelium, Female, Gene Expression Regulation, Multigene Family, Glutathione Peroxidase, Humans, Mice, Inbred BALB C, Molecular Sequence Data, Molecular Weight, Organ Specificity, Wound Healing, Keratinocytes, Mice

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