The DOI matches its Crossref record; 45 of the 46 references it cites were found in a registry or an index.
Insulin-like growth factor (IGF-I) induces myotube hypertrophy associated with an increase in anaerobic glycolysis in a clonal skeletal-muscle cell model
Open accessAbstract
Insulin-like growth factor-I (IGF-I) is an important autocrine/paracrine mediator of skeletal-muscle growth and development. To develop a definitive cultured cell model of skeletal-muscle hypertrophy, C2C12 cells were stably transfected with IGF-I and clonal lines developed and evaluated. Quantitative morphometric analysis showed that IGF-I-transfected myotubes had a larger area (2381±60 µm2 versus 1429±39 µm2; P< 0.0001) and a greater maximum width (21.4±0.6 µm versus 13.9±0.3 µm; P< 0.0001) than control C2C12 myotubes, independent of the number of cell nuclei per myotube. IGF-I-transfected myotubes had higher levels of protein synthesis but no difference in DNA synthesis when compared with control myotubes, indicating the development of hypertrophy rather than hyperplasia. Both lactate dehydrogenase and alanine aminotransferase activities were increased (3- and 5-fold respectively), and total lactate levels were higher (2.3-fold) in IGF-I-transfected compared with control myotubes, indicating an increase in anaerobic glycolysis in the hypertrophied myotubes. However, expression of genes involved in skeletal-muscle growth or hypertrophy in vivo, e.g. myocyte nuclear factor and myostatin, was not altered in the IGF-I myotubes. Finally, myotube hypertrophy could also be induced by treatment of C2C12 cells with recombinant IGF-I or by growing C2C12 cells in conditioned media from IGF-I-transfected cells. This quantitative model should be uniquely useful for elucidating the molecular mechanisms of skeletal-muscle hypertrophy.
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“IGF-1 (100 ng/mL), employed as positive control (30) displayed, as expected, an improvement in [ 3 H]-Leu incorporation (+20%, p < 0.001).”
From 20-Hydroxyecdysone activates the protective arm of the renin angiotensin system via Mas receptor (2020) · cites this paper as [30]
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Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
Regulation of skeletal muscle mass in mice by a new TGF-p superfamily member
McPherron, A. C., Lawler, A. M., & Lee, S.-J. (1997) · Nature · doi:10.1038/387083a0 · Cited by 4,013
Liu, J.-P., Baker, J. C., Perkins, A. S., Robertson, E. J., & Efstratiadis, A. (1993) · Cell · doi:10.1016/s0092-8674(05)80084-4 · Cited by 2,854
G1 phase progression: Cycling on cue
Sherr, C. J. (1994) · Cell · doi:10.1016/0092-8674(94)90540-1 · Cited by 2,675
Role of insulin-like growth factors in embryonic and postnatal growth
Baker, J., Liu, J.-P., Robertson, E. J., & Efstratiadis, A. (1993) · Cell · doi:10.1016/s0092-8674(05)80085-6 · Cited by 2,463
Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes
Ullrich, A., Bell, J. R., Chen, E. Y., Herrera, R., Petruzzelli, L., Dull, T. J., Gray, A., Coussens, L. M., Liao, Y.-C., Tsubokawa, M., et al. (1985) · Nature · doi:10.1038/313756a0 · Cited by 2,251
G 1 Events and Regulation of Cell Proliferation
Pardee, A. B. (1989) · Science · doi:10.1126/science.2683075 · Cited by 2,182
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle
Yaffe, D., & Saxel, O. (1977) · Nature · doi:10.1038/270725a0 · Cited by 2,153
A deletion in the bovine myostatin gene causes the double–muscled phenotype in cattle
Grobet, L., Royo Martin, L. J., Poncelet, D., Pirottin, D., Brouwers, B., Riquet, J., Schoeberlein, A., Dunner, S., Ménissier, F., Massabanda, J., et al. (1997) · Nature Genetics · doi:10.1038/ng0997-71 · Cited by 1,556
Growth Hormone and the Insulin-Like Growth Factor System in Myogenesis*
Florini, J. R., Ewton, D. Z., & Coolican, S. A. (1996) · Endocrine Reviews · doi:10.1210/edrv-17-5-481 · Cited by 1,148
Research works printed but not linked: 1
- 12 Rosenthal, S. M. and Cheng, Z. Q. (1995) Proc. Natl. Acad. Sci. U.S.A. 92 , 10307-10311
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Rommel, C., Bodine, S. C., Clarke, B. A., Rossman, R., Nunez, L., Stitt, T. N., Yancopoulos, G. D., & Glass, D. J. (2001) · Nature Cell Biology · doi:10.1038/ncb1101-1009 · Cited by 1,583
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Legate, K., Wickström, S., & Fässler, R. (2009) · Genes & Development · doi:10.1101/gad.1758709 · Cited by 835
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Semsarian, C., Wu, M., Ju, Y., Marciniec, T., Yeoh, T., Allen, D. G., Harvey, R. P., & Graham, R. M. (1999) · Nature · doi:10.1038/23054 · Cited by 451
Self-citationMyostatin inhibits cell proliferation and protein synthesis in C2C12 muscle cells
Taylor, W. E., Bhasin, S., Artaza, J., Byhower, F., Azam, M., Willard, D. H., Kull, F. C., & González-Cadavid, N. F. (2001) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.2001.280.2.e221 · Cited by 410
Metadata
- Authors
- SEMSARIAN, C., SUTRAVE, P., RICHMOND, D. R., & GRAHAM, R. M.
- Affiliations
- Victor Chang Cardiac Research Institute (AU)
- Royal Prince Alfred Hospital (AU)
- Corresponding author
- SEMSARIAN, Christopher
- SUTRAVE, Pramod
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1999-04-08
- Volume
- 339
- Issue
- 2
- Pages
- 443-451
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1220176
- OpenAlex
- W2120432317
- Cited by
- 106
- References
- 46
- Citations by year
- 2026: 3 · 2024: 2 · 2023: 4 · 2022: 2 · 2021: 4 · 2020: 4 · 2018: 4 · 2017: 3 · 2016: 4 · 2015: 4 · 2014: 1 · 2013: 3 · 2012: 1
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Australian Government
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
- Keywords
- insulin-like growth factor I, skeletal muscle hypertrophy, myotube hypertrophy, anaerobic glycolysis, C2C12 myoblasts, stable transfection, protein synthesis, lactate dehydrogenase, alanine aminotransferase, myostatin, muscle growth
- MeSH terms
- Animals, Base Sequence, Cell Differentiation, Cell Division, Cell Line, DNA Replication, Glycolysis, Hypertrophy, Insulin-Like Growth Factor I, Models, Biological, RNA, Messenger, Transfection, DNA Primers, Muscle, Skeletal, Mice
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