The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Data from amplified fragment length polymorphism (AFLP) markers show indication of size homoplasy and of a relationship between degree of homoplasy and fragment size
Abstract
We investigate the distribution of sizes of fragments obtained from the amplified fragment length polymorphism (AFLP) marker technique. We find that empirical distributions obtained in two plant species, Phaseolus lunatus and Lolium perenne, are consistent with the expected distributions obtained from analytical theory and from numerical simulations. Our results indicate that the size distribution is strongly asymmetrical, with a much higher proportion of small than large fragments, that it is not influenced by the number of selective nucleotides nor by genome size but that it may vary with genome‐wide GC‐content, with a higher proportion of small fragments in cases of lower GC‐content when considering the standard AFLP protocol with the enzyme Mse I. Results from population samples of the two plant species show that there is a negative relationship between AFLP fragment size and fragment population frequency. Monte Carlo simulations reveal that size homoplasy, arising from pulling together nonhomologous fragments of the same size, generates patterns similar to those observed in P. lunatus and L. perenne because of the asymmetry of the size distribution. We discuss the implications of these results in the context of estimating genetic diversity with AFLP markers.
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The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis
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Cited by
Genotyping errors: causes, consequences and solutions
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Ten years of AFLP in ecology and evolution: why so few animals?
BENSCH, S., & ÅKESSON, M. (2005) · Molecular Ecology · doi:10.1111/j.1365-294x.2005.02655.x · Cited by 675
Almost Forgotten or Latest Practice? AFLP applications, analyses and advances
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Analysis of multilocus fingerprinting data sets containing missing data
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Metadata
- Authors
- Vekemans, X., Beauwens, T., Lemaire, M., & Roldán‐Ruiz, I.
- Venue
- Molecular Ecology
- Publisher
- Wiley
- Published
- 2002-01
- Volume
- 11
- Issue
- 1
- Pages
- 139-151
- ISSN
- 0962-1083 (ISSN-L)
- 1365-294X
- OpenAlex
- W2127075690
- Cited by
- 800
- References
- 21
- FWCI
- 8.84
- Type
- journal article
- Language
- en
- Open access
- closed
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- Vekemans, X., Beauwens, T., Lemaire, M., & Roldán‐Ruiz, I. (2002). Data from amplified fragment length polymorphism (AFLP) markers show indication of size homoplasy and of a relationship between degree of homoplasy and fragment size. Molecular Ecology. https://doi.org/10.1046/j.0962-1083.2001.01415.x
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