The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Rapid detection of α‐thalassaemia deletions and α‐globin gene triplication by multiplex polymerase chain reactions
Abstract
We describe a sensitive, reliable and reproducible method, based on three multiplex PCR assays, for the rapid detection of seven common α‐thalassaemia deletions and one α‐globin gene triplication. The new assay detects the α 0 deletions – –SEA, – (α)20.5, – –MED, – –FIL and – –THAI in the first multiplex PCR, the second multiplex detects the –α 3.7 deletion and ααα anti3.7 variant, the third multiplex detects the –α 4.2 deletion. This simple multiplex method should greatly facilitate the genetic screening and molecular diagnosis of these determinants in populations where α‐thalassaemias are prevalent.
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Cites
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Cited by
Harteveld, C. L., & Higgs, D. R. (2010) · Orphanet Journal of Rare Diseases · doi:10.1186/1750-1172-5-13 · Cited by 508
A rapid and reliable 7-deletion multiplex polymerase chain reaction assay for α-thalassemia
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Xu, X. M., Zhou, Y. Q., Luo, G. X., Liao, C., Zhou, M., Chen, P. Y., Lu, J. P., Jia, S. Q., Xiao, G. F., Shen, X., et al. (2004) · Journal of Clinical Pathology · doi:10.1136/jcp.2003.014456 · Cited by 324
Henderson, S. J., Timbs, A. T., McCarthy, J., Gallienne, A. E., Proven, M., Rugless, M. J., Lopez, H., Eglinton, J., Dziedzic, D., Beardsall, M., et al. (2016) · Hemoglobin · doi:10.3109/03630269.2015.1113990 · Cited by 51
Puehringer, H., Najmabadi, H., Law, H.-Y., Krugluger, W., Viprakasit, V., Pissard, S., Baysal, E., Taher, A., Farra, C., Al-Ali, A., et al. (2007) · Clinical Chemical Laboratory Medicine · doi:10.1515/cclm.2007.125 · Cited by 45
Metadata
- Authors
- Liu, Old, Miles, Fisher, Weatherall, & Clegg
- Venue
- British Journal of Haematology
- Publisher
- Wiley
- Published
- 2000-02
- Volume
- 108
- Issue
- 2
- Pages
- 295-299
- ISSN
- 0007-1048 (ISSN-L)
- 1365-2141
- OpenAlex
- W2083048402
- Cited by
- 360
- References
- 12
- FWCI
- 5.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- Liu, Old, Miles, Fisher, Weatherall, & Clegg. (2000). Rapid detection of α‐thalassaemia deletions and α‐globin gene triplication by multiplex polymerase chain reactions. British Journal of Haematology. https://doi.org/10.1046/j.1365-2141.2000.01870.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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