The DOI matches its Crossref record.
Betaine Improves the PCR Amplification of GC-Rich DNA Sequences
Open accessAbstract
Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.
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Cited by
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A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring
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Metadata
- Authors
- Henke, W., Herdel, K., Jung, K., Schnorr, D., & Loening, S. A.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 1997-10-01
- Volume
- 25
- Issue
- 19
- Pages
- 3957-3958
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2127457293
- Cited by
- 510
- References
- 0
- FWCI
- 2.70
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Henke, W., Herdel, K., Jung, K., Schnorr, D., & Loening, S. A. (1997). Betaine Improves the PCR Amplification of GC-Rich DNA Sequences. Nucleic Acids Research. https://doi.org/10.1093/nar/25.19.3957
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
