The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Expansion of cytotoxic CD8 + CD28 − T cells in healthy ageing people, including centenarians
Abstract
Ageing is associated with complex remodelling in the phenotypic and functional profiles of T lymphocytes. We investigated whether expression of CD28 antigen on T cells is conserved throughout adulthood and ageing in humans. For this purpose we analysed T cells obtained from peripheral blood of 102 healthy people of ages ranging from 20 to 105 years. We found an age‐related increase of CD28 − T cells in percentage and absolute number, predominantly among CD8 + T cells. CD28 − T cells from aged donors analysed by flow cytometry appeared as resting cells (not expressing CD25, CD38, CD69, CD71, DR), bearing markers of cytotoxic activity (CD11b and CD57) and with a phenotype compatible with ‘memory’ cells (up‐regulated CD2 and CD11a; CD62L absent). At the functional level, freshly isolated purified CD28 − CD8 + T cells showed high anti‐CD3 redirected cytotoxic activity against Fc‐bearing P815 cells. The same activity tested on freshly isolated bulk T lymphocytes was significantly augmented with age. We found a positive correlation between age, number of CD8 + CD28 − T cells and anti‐CD3 redirected cytotoxicity by freshly isolated T cells. These data suggest that an activation of unknown nature within the cytotoxic arm of the immune system occurs with age. We speculate that these cytotoxic T lymphocytes (CTL) in vivo may constitute armed effector cells for immediate killing of targets bearing peptides from pathogens of intracellular origin.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“An established hallmark of adaptive immune ageing is the expansion of the memory population of highly cytotoxic CD8+CD28− T cells114”
From NAD+ metabolism and its roles in cellular processes during ageing (2021) · cites this paper as [114]
Citations
Cites
Human Aging: Usual and Successful
Rowe, J. W., & Kahn, R. L. (1987) · Science · doi:10.1126/science.3299702 · Cited by 3,384
Schwartz, R. H. (1992) · Cell · doi:10.1016/s0092-8674(05)80055-8 · Cited by 1,293
Linsley, P. S., Brady, W., Grosmaire, L. S., Aruffo, A. A., Damle, N. K., & Ledbetter, J. A. (1991) · The Journal of Experimental Medicine · doi:10.1084/jem.173.3.721 · Cited by 1,126
Sanders, M. E., Makgoba, M. W., Sharrow, S. O., Stephany, D., Springer, T. A., Young, H. A., & Shaw, S. (1988) · The Journal of Immunology · doi:10.4049/jimmunol.140.5.1401 · Cited by 906
Signals and signs for lymphocyte responses
Janeway, C. A., & Bottomly, K. (1994) · Cell · doi:10.1016/0092-8674(94)90335-2 · Cited by 849
Posnett, D. N., Sinha, R., Kabak, S., & Russo, C. (1994) · The Journal of Experimental Medicine · doi:10.1084/jem.179.2.609 · Cited by 761
Role of the CD28 receptor in T-cell activation
June, C. H., Ledbetter, J. A., Linsley, P. S., & Thompson, C. B. (1990) · Immunology Today · doi:10.1016/0167-5699(90)90085-n · Cited by 674
The immunology of exceptional individuals: the lesson of centenarians
Franceschi, C., Monti, D., Sansoni, P., & Cossarizza, A. (1995) · Immunology Today · doi:10.1016/0167-5699(95)80064-6 · Cited by 535
Self-citationCD8 naive T cell counts decrease progressively in HIV-infected adults.
Roederer, M., Dubs, J. G., Anderson, M. T., Raju, P. A., Herzenberg, L. A., & Herzenberg, L. A. (1995) · Journal of Clinical Investigation · doi:10.1172/jci117892 · Cited by 489
Sansoni, P., Cossarizza, A., Brianti, V., Fagnoni, F. F., Snelli, G., Monti, D., Marcato, A., Passeri, G., Ortolani, C., & Forti, E. (1993) · Blood · doi:10.1182/blood.v82.9.2767.2767 · Cited by 457
Self-citation
Cited by
Sorted by citations
NAD+ metabolism and its roles in cellular processes during ageing
Covarrubias, A. J., Perrone, R., Grozio, A., & Verdin, E. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00313-x · Cited by 1,617
Phenotypic and functional separation of memory and effector human CD8+ T cells
Hamann, D., Baars, P. A., Rep, M. H. G., Hooibrink, B., Kerkhof-Garde, S. R., Klein, M. R., & Lier, R. A. W. V. (1997) · The Journal of Experimental Medicine · doi:10.1084/jem.186.9.1407 · Cited by 1,366
An epigenetic clock analysis of race/ethnicity, sex, and coronary heart disease
Horvath, S., Gurven, M., Levine, M. E., Trumble, B. C., Kaplan, H. S., Allayee, H., Ritz, B. R., Chen, B. H., Lu, A. T.-H., Rickabaugh, T. M., et al. (2016) · Genome biology · doi:10.1186/s13059-016-1030-0 · Cited by 749
CD28− T cells: their role in the age-associated decline of immune function
Weng, N., Akbar, A. N., & Goronzy, J. J. (2009) · Trends in Immunology · doi:10.1016/j.it.2009.03.013 · Cited by 630
Immunosenescence and inflammaging in the aging process: age-related diseases or longevity?
Santoro, A., Bientinesi, E., & Monti, D. (2021) · Ageing Research Reviews · doi:10.1016/j.arr.2021.101422 · Cited by 603
Metadata
- Authors
- FAGNONI, F. F., VESCOVINI, R., MAZZOLA, M., BOLOGNA, G., NIGRO, E., LAVAGETTO, G., FRANCESCHI, C., PASSERI, M., & SANSONI, P.
- Affiliations
- University of Parma (IT)
- University of Modena and Reggio Emilia (IT)
- Corresponding author
- SANSONI, P.
- ORCID
- FRANCESCHI, C.
- Venue
- Immunology
- Publisher
- Wiley
- Published
- 1996-08
- Volume
- 88
- Issue
- 4
- Pages
- 501-507
- ISSN
- 0019-2805 (ISSN-L)
- 1365-2567
- OpenAlex
- W2030414136
- Cited by
- 362
- References
- 36
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 13 · 2023: 12 · 2022: 15 · 2021: 13 · 2020: 14 · 2019: 13 · 2018: 15 · 2017: 23 · 2016: 12 · 2015: 8 · 2014: 13 · 2013: 9 · 2012: 5
- FWCI
- 4.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Centre National de la Recherche Scientifique
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Immune Cell Function and Interaction
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- CD28-negative T cells, CD8+ T cells, cytotoxic T lymphocytes, aging, centenarians, T cell activation, cytotoxicity, T cell memory phenotype, CD11b, CD57, age-related immune changes
- MeSH terms
- Adult, Aged, Aged, 80 and over, Aging, Cell Separation, Cytotoxicity, Immunologic, Flow Cytometry, Humans, Middle Aged, CD3 Complex, CD28 Antigens, CD8-Positive T-Lymphocytes, Lymphocyte Count
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
