The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Microbial colonization influences composition and T‐cell receptor Vβ repertoire of intraepithelial lymphocytes in rat intestine
Abstract
Studies in mice have shown that the composition of intestinal intraepithelial lymphocytes (IEL) may be markedly altered by gut microbial colonization. Such modulation was studied in a rat model by the use of germ-free and conventionalized animals from which IEL from the small intestine were isolated and analysed by flow cytometry. Conventionalization caused expansion as well as phenotypic alterations of T-cell receptor (TCR) alpha/beta + IEL in that the proportions of CD4+ and CD8 alpha beta + TCR alpha/beta + cells were increased, while the double negative (CD4- CD8-) fraction was reduced. microbial colonization also influenced the TCR V beta repertoire of CD8+ IEL in that the proportions of V beta 8.2+ and V beta 10+ cells were increased, whereas V beta 8.5+ and V beta 16+ cells were relatively decreased. Moreover, conventionalization influenced the levels of TCR cell surface expression in the same V beta subsets. Three-colour flow-cytometric analysis demonstrated that skewing of the V beta repertoire was most pronounced in the CD8 alpha alpha + subset, although the numerical increase of IEL mainly included the CD8 alpha beta + subset. In contrast to IEL, the TCR V beta repertoire in mesenteric lymph nodes was unchanged after intestinal colonization. These results confirm that TCR alpha/beta + IEL subpopulations respond dynamically to the microbial gut flora and suggest that their V beta repertoire can be shaped by luminal microbial antigens.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The minimalist bacterial colonization of the ASF mouse avoids the complications of the high abundance and diversity in conventional mice, while overcoming the host developmental hurdles seen in GF mice, such as an underdeveloped immune system (Atarashi et al., 2011;Helgeland et al., 1996), slower intestinal epithelial turnover (Savage et al., 1981), differing nutritional requirements and less body”
From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Helgeland et al., 1996
Citations
Cites
CD1-Restricted T Cell Recognition of Microbial Lipoglycan Antigens
Sieling, P. A., Chatterjee, D., Porcelli, S. A., Prigozy, T. I., Mazzaccaro, R. J., Soriano, T. T., Bloom, B. R., Brenner, M. B., Kronenberg, M., Brennan, P. J., et al. (1995) · Science · doi:10.1126/science.7542404 · Cited by 727
Guy‐Grand, D., Cerf–Bensussan, N., MALISSEN, B., Malassis-Séris, M., Briottet, C., & VASSALLI, P. (1991) · The Journal of Experimental Medicine · doi:10.1084/jem.173.2.471 · Cited by 595
Hünig, T., Wallny, H., Hartley, J. K., Lawetzky, A., & Tiefenthaler, G. (1989) · The Journal of Experimental Medicine · doi:10.1084/jem.169.1.73 · Cited by 451
Bandeira, A., Mota‐Santos, T. A., Itohara, S., Degermann, S., Heusser, C., Tonegawa, S., & Coutinho, A. (1990) · The Journal of Experimental Medicine · doi:10.1084/jem.172.1.239 · Cited by 283
Umesaki, Y., Setoyama, H., Matsumoto, S., & Okada, Y. (1993) · PubMed Central · Cited by 270
Jarry, A., Cerf–Bensussan, N., Brousse, N., Selz, F., & Guy‐Grand, D. (1990) · European Journal of Immunology · doi:10.1002/eji.1830200523 · Cited by 266
Phenotypic complexity of intraepithelial lymphocytes of the small intestine
Lefrançois, L. (1991) · The Journal of Immunology · doi:10.4049/jimmunol.147.6.1746 · Cited by 249
Oligoclonal Expansion and CD1 Recognition by Human Intestinal Intraepithelial Lymphocytes
Balk, S. P., Ebert, E. C., Blumenthal, R. L., McDermott, F. V., Wucherpfennig, K. W., Landau, S. B., & Blumberg, R. S. (1991) · Science · doi:10.1126/science.1716785 · Cited by 235
Diversity of γδ T-cell receptors on murine intestinal intraepithelial lymphocytes
Takagaki, Y., DeCloux, A., Bonneville, M., & Tonegawa, S. (1989) · Nature · doi:10.1038/339712a0 · Cited by 217
H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes
Pamer, E. G., Wang, C.-R., Flaherty, L. A., Lindahl, K. F., & Bevan, M. J. (1992) · Cell · doi:10.1016/0092-8674(92)90097-v · Cited by 199
Cited by
Sorted by citations
Cryan, J. F., O'Riordan, K. J., Cowan, C. S. M., Sandhu, K. V., Bastiaanssen, T. F. S., Boehme, M., Codagnone, M. G., Cussotto, S., Fulling, C., Golubeva, A. V., et al. (2019) · Physiological Reviews · doi:10.1152/physrev.00018.2018 · Cited by 5,286
Gut flora in health and disease
Guarner, F., & Malagelada, J. (2003) · The Lancet · doi:10.1016/s0140-6736(03)12489-0 · Cited by 3,480
Epithelial-cell recognition of commensal bacteria and maintenance of immune homeostasis in the gut
Artis, D. (2008) · Nature reviews. Immunology · doi:10.1038/nri2316 · Cited by 1,136
Probiotic bacteria enhance murine and human intestinal epithelial barrier function
Madsen, K., Cornish, A. S., Soper, P., McKaigney, C. J., Jijon, H. B., Yachimec, C., Doyle, J. S. G., Jewell, L. D., & Simone, C. D. (2001) · Gastroenterology · doi:10.1053/gast.2001.27224 · Cited by 1,096
Probiotics: an overview of beneficial effects
Ouwehand, A. C., Salminen, S., & Isolauri, E. (2002) · Antonie van Leeuwenhoek · doi:10.1023/a:1020620607611 · Cited by 1,066
Metadata
- Authors
- HELGELAND, L., VAAGE, J. T., ROLSTAD, B., MIDTVEDT, T., & BRANDTZAEG, P.
- Affiliations
- University of Oslo (NO)
- Karolinska Institutet (SE)
- Pathologisches Institut (DE)
- Corresponding author
- HELGELAND, L.
- Venue
- Immunology
- Publisher
- Wiley
- Published
- 1996-12
- Volume
- 89
- Issue
- 4
- Pages
- 494-501
- ISSN
- 0019-2805 (ISSN-L)
- 1365-2567
- OpenAlex
- W2040231886
- Cited by
- 122
- References
- 37
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 3 · 2023: 1 · 2021: 3 · 2020: 4 · 2019: 2 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 8 · 2013: 3 · 2012: 8
- FWCI
- 1.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Norges Forskningsråd
- Medical Research Council
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- intraepithelial lymphocytes, gut microbiota, microbial colonization, T cell receptor V beta repertoire, germ-free rats, conventionalization, CD4+ T cells, double-negative T cells, mesenteric lymph nodes, small intestine
- MeSH terms
- Animals, Flow Cytometry, Germ-Free Life, Intestinal Mucosa, Lymphocyte Activation, Male, Mesentery, Rats, Inbred Strains, T-Lymphocytes, CD4-Positive T-Lymphocytes, Receptors, Antigen, T-Cell, alpha-beta, CD8-Positive T-Lymphocytes, Immunity, Mucosal, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
