The DOI matches its Crossref record; 177 of the 177 references it cites were found in a registry or an index.
The current status of primary hepatocyte culture
Open accessAbstract
Recently, there have been significant advances toward the development of culture conditions that promote proliferation of primary rodent hepatocytes. There are two major methods for the multiplication of hepatocytes in vitro: one is the use of nicotinamide, the other is the use of a nutrient-rich medium. In the medium containing a high concentration of nicotinamide and a growth factor, primary hepatocytes can proliferate well. In this culture condition small mononucleate cells, which are named small hepatocytes, appear and form colonies. Small hepatocytes have a high potential to proliferate while maintaining hepatic characteristics, and can differentiate into mature ones. On the other hand, combining the nutrient-rich medium with 2% DMSO, the proliferated hepatocytes can recover the hepatic differentiated functions and maintain them for a long time. In this review I describe the culture conditions for the proliferation and differentiation of primary hepatocytes and discuss the small hepatocytes, especially their roles in liver growth.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“For decades, expandable HHs have been a bottleneck for their applications for research purposes and clinical therapies (Bhatia et al., 2014; Michalopoulos, 2014; Mitaka, 1998; Runge et al., 2000”
From In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity (2018) · cites this paper as Mitaka, 1998
In the citing paper
“However, the actual use of hepatocyte transplantation has been hampered by the limited availability of donor hepatocytes and the inability to expand primary HHs (PHHs) in vitro.”
From In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity (2018) · cites this paper as Mitaka, 1998
Citations
Cites
Experimental pathology of the liver
Higgins, G. (1931) · Medical Entomology and Zoology · Cited by 4,057
Higgins, G. (1931) · Cited by 2,444
Bloom, W., & Fawcett, D. W. (1962) · Cited by 2,210
Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid
Breitman, T. R., Selonick, S. E., & Collins, S. J. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.5.2936 · Cited by 1,966
Engineering cell shape and function
Singhvi, R., Kumar, A., Lopez, G. P., Stephanopoulos, G. N., Wang, D. I. C., Whitesides, G. M., & Ingber, D. E. (1994) · Science · doi:10.1126/science.8171320 · Cited by 1,453
Control of proliferation in animal cells
Clarkson, B. D., & Baserga, R. (1974) · Cold Spring Harbor Laboratory eBooks · Cited by 1,196
Nakamura, T., Nawa, K., & Ichihara, A. (1984) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(84)91253-1 · Cited by 1,134
Stillman, B. (1995) · Molecular Medicine · doi:10.1007/bf03401885 · Cited by 1,031
Liver regeneration: molecular mechanisms of growth control
Michalopoulos, G. K. (1990) · The FASEB Journal · doi:10.1096/fasebj.4.2.2404819 · Cited by 897
Gohda, E., Tsubouchi, H., Nakayama, H., Hirono, S., Sakiyama, O., Takahashi, K., MIYAZAKI, H., Hashimoto, S., & Daikuhara, Y. (1988) · Journal of Clinical Investigation · doi:10.1172/jci113334 · Cited by 656
Cited by
Sorted by citations
Items 101–114 of 114
Three-Dimensional Culture of Fetal Mouse, Rat, and Porcine Hepatocytes
Sakai, Y., Jiang, J., Hanada, S., Huang, H., Katsuda, T., Kojima, N., Teratani, T., Miyajima, A., & Ochiya, T. (2012) · Springer eBooks · doi:10.1007/978-1-4471-4171-6_4 · Cited by 1
Tissue engineering of organ systems
Hodges, S. J., Frey, P., & Atala, A. (2008) · Elsevier eBooks · doi:10.1016/b978-0-12-370869-4.00021-5
THREE-DIMENSIONAL CULTURE OF FETAL LIVER CELLS
Qian, L. (2006) · eCommons (Cornell University)
Hu, W., & Bernhagen, J. (2013) · RWTH Publications (RWTH Aachen)
Ramakrishnan, V. M. (2015) · ThinkIR: The University of Louisville's Institutional Repository (University of Louisville) · doi:10.18297/etd/2319
Culture with Growth Factor Supplements Improves the Viability and Function of Rat Hepatocytes
Na, G. H., Kim, D. G., & Jung, E. S. (2015) · Korean Journal of Transplantation · doi:10.4285/jkstn.2015.29.3.139
Discrete Modelling of Liver Cell Aggregation Using Partial Differential Equations
Geroski, T., Nikolic, M. M., Vrana, N. E., & Filipović, N. (2019) · World Congress on Medical Physics and Biomedical Engineering, September 7 - 12, 2009, Munich, Germany · doi:10.1007/978-3-030-17971-7_57
Evaluation of a multiwell perfused bioreactor system for the long-term culture of mouse hepatocytes
Soldatow, V. Y. (2019) · Carolina Digital Repository (University of North Carolina at Chapel Hill) · doi:10.17615/2zbz-s872
Engenharia tecidual hepática utilizando células tronco pluripotentes induzidas
Guimarães, E. D. S. G. (2019) · doi:10.11606/t.41.2019.tde-27082019-093823
Ogawa, K., & Vacanti, J. P. (2002) · Elsevier eBooks · doi:10.1016/b978-012436636-7/50202-0
Fan, J., Dama, G., Liu, Y. L., Guo, W., & Lin, J. (2023) · Molecular Biology · doi:10.31857/s0026898423040031
Small hepatocytes in primary cultures
Mitaka, T., & Sato, F. (2000) · Springer eBooks · doi:10.1007/978-94-017-3345-8_14
Self-citationDrexler, H. (2002) · doi:10.1290/1071-2690(2002)038
Self-citation
Metadata
- Authors
- Mitaka, T.
- Affiliations
- Sapporo Medical University (JP)
- Corresponding author
- Mitaka, Toshihiro
- ORCID
- Mitaka, Toshihiro
- Venue
- International Journal of Experimental Pathology
- Publisher
- Wiley
- Published
- 1998-12
- Volume
- 79
- Issue
- 6
- Pages
- 393-409
- ISSN
- 0959-9673 (ISSN-L)
- 1365-2613
- PMC
- PMC3220367
- OpenAlex
- W1908212314
- Cited by
- 115
- References
- 177
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 4 · 2019: 9 · 2018: 6 · 2017: 5 · 2016: 4 · 2015: 8 · 2014: 3 · 2013: 7 · 2012: 4
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- International Journal of Experimental Pathology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Surgery › Organ Transplantation Techniques and Outcomes
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Keywords
- primary hepatocyte culture, hepatocyte proliferation, nicotinamide, dimethyl sulfoxide, hepatocyte differentiation, liver growth
- MeSH terms
- Animals, Cell Differentiation, Cell Division, Culture Media, Liver, Cell Culture Techniques, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
