Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats
Abstract
When rat serum was subjected to gel filtration on a Sephadex G-200 column, a factor, "hepatotropin", that promoted hepatocyte growth in primary culture was separated. Its Mr was about 150 KD and it was an anionic protein that was unstable on acid- and heat-treatments. Hepatotropin was purified 20-fold further by affinity chromatography on heparin-Sepharose CL-6B. The purified hepatotropin was effective at 20 micrograms/ml and maximally effective at 120 micrograms/ml, and its effect was additive with that of insulin plus epidermal growth factor. In rats after partial hepatectomy, the hepatotropin activity in the serum increased time-dependently reaching a maximum of about 5-fold the initial level 24 h after the operation. Various known growth factors, such as fibroblast growth factor, platelet derived growth factor, somatomedin, thrombin and transferrin, did not stimulate DNA synthesis in cultured hepatocytes. These results suggest that hepatotropin is a new growth factor.
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High doses of HGF can suppress mitosis or induce apoptosis.
“Since high doses of HGF can suppress mitosis [19] or induce apoptosis [20]”
From Synthesis of IL-6 by Hepatocytes Is a Normal Response to Common Hepatic Stimuli (2014) · cites this paper as [19]
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“Since high doses of HGF can suppress mitosis [19] or induce apoptosis [20], for control purposes we also tested a non-mitogenic dose of HGF (500 ng/ml, efficacy confirmed by lack of thymidine incorporation, data not shown).”
MethodThe current status of primary hepatocyte culture
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- Authors
- Nakamura, T., Nawa, K., & Ichihara, A.
- Affiliations
- Tokushima University (JP)
- ORCID
- Nakamura, Toshikazu
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1984-08
- Volume
- 122
- Issue
- 3
- Pages
- 1450-1459
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2048367533
- Cited by
- 1,134
- References
- 1
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 7 · 2023: 9 · 2022: 17 · 2021: 8 · 2020: 26 · 2019: 10 · 2018: 17 · 2017: 21 · 2016: 8 · 2015: 29 · 2014: 22 · 2013: 25 · 2012: 33
- FWCI
- 12.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fibroblast Growth Factor Research
- Health Sciences › Medicine › Hepatology › Hepatocellular Carcinoma Treatment and Prognosis
- Keywords
- hepatocyte growth factor, partial hepatectomy, serum growth factors, hepatocyte proliferation, anionic proteins
- MeSH terms
- Animals, Blood Platelets, Cells, Cultured, Chromatography, Affinity, Chromatography, Gel, DNA Replication, Growth Substances, Liver, Liver Regeneration, Rats
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