Cited by 115Liver physiology and pathologyOrgan Transplantation Techniques and OutcomesDrug-Induced Hepatotoxicity and Protection

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The current status of primary hepatocyte culture

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Mitaka, Toshihiro·1998·International Journal of Experimental Pathology

DOI 10.1046/j.1365-2613.1998.00083.xPMC PMC3220367

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Abstract

Recently, there have been significant advances toward the development of culture conditions that promote proliferation of primary rodent hepatocytes. There are two major methods for the multiplication of hepatocytes in vitro: one is the use of nicotinamide, the other is the use of a nutrient-rich medium. In the medium containing a high concentration of nicotinamide and a growth factor, primary hepatocytes can proliferate well. In this culture condition small mononucleate cells, which are named small hepatocytes, appear and form colonies. Small hepatocytes have a high potential to proliferate while maintaining hepatic characteristics, and can differentiate into mature ones. On the other hand, combining the nutrient-rich medium with 2% DMSO, the proliferated hepatocytes can recover the hepatic differentiated functions and maintain them for a long time. In this review I describe the culture conditions for the proliferation and differentiation of primary hepatocytes and discuss the small hepatocytes, especially their roles in liver growth.

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Authors: Mitaka, T.
Affiliations: Sapporo Medical University (JP)
Corresponding author: Mitaka, Toshihiro
ORCID: Mitaka, Toshihiro
Venue: International Journal of Experimental Pathology
Publisher: Wiley
Published: 1998-12
Volume: 79
Issue: 6
Pages: 393-409
ISSN: 0959-9673 (ISSN-L)
1365-2613
DOI: 10.1046/j.1365-2613.1998.00083.x
PMC: PMC3220367
OpenAlex: W1908212314
Cited by: 115
References: 177
Citations by year: 2026: 1 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 4 · 2019: 9 · 2018: 6 · 2017: 5 · 2016: 4 · 2015: 8 · 2014: 3 · 2013: 7 · 2012: 4
FWCI: 2.51
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor, 1235 d)
Locations: International Journal of Experimental Pathology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Health Sciences › Medicine › Surgery › Organ Transplantation Techniques and Outcomes
Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
Keywords: primary hepatocyte culture, hepatocyte proliferation, nicotinamide, dimethyl sulfoxide, hepatocyte differentiation, liver growth
MeSH terms: Animals, Cell Differentiation, Cell Division, Culture Media, Liver, Cell Culture Techniques, Rats

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