Evaluation of potential biocontrol rhizobacteria from different host plants of Verticillium dahliae Kleb
Abstract
AIMS: A screening approach was developed to assess the potential of rhizobacterial strains to control Verticillium wilt caused by Verticillium dahliae Kleb. METHODS AND RESULTS: Sixty randomly chosen antagonistic bacterial strains originally isolated from rhizosphere of three different host plants of V. dahliae--strawberry, potato and oilseed rape--were evaluated for biocontrol and plant growth promotion by analysing in vitro antagonism towards V. dahliae and other plant pathogenic fungi, production of fungal cell wall-degrading enzymes and plant growth-promoting effects on strawberry seedlings. To test the plant growth-promoting effect, a microplate assay with strawberry seedlings was developed. Although the rhizobacterial strains were isolated from different plants they showed effects on the growth of strawberry seedlings. According to the in vitro biocontrol and plant growth-promoting activity, the three best candidates Pseudomonas putida B E2 (strawberry rhizosphere), Ps. chlororaphis K15 (potato rhizosphere) and Serratia plymuthica R12 (oilseed rape rhizosphere) were selected for greenhouse experiments to verify the in vitro screening results. Under greenhouse conditions the isolates selected according to this strategy were as effective, or more effective than commercial biocontrol agents and may therefore possibly be valuable as antagonists of V. dahliae. CONCLUSIONS: In this study, the screening strategy resulted in a selection of three interesting biocontrol candidates against Verticillium: Ps. putida B E2 (strawberry rhizosphere), Ps. chlororaphis K15 (potato rhizosphere) and Ser. plymuthica R12 (oilseed rape rhizosphere). SIGNIFICANCE AND IMPACT OF THE STUDY: A new combination of in vitro screening methods including a microplate assay with strawberry seedlings to test the plant growth promoting effect which allow to more efficiently select potential biological control agents was developed successfully.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Microorganisms can produce a wide array of compounds with antimicrobial activity and hydrolytic enzymes catalyzing cell wall lysis, and they will control growth and activity of pathogenic fungi.
“Since microorganisms can produce a wide array of compounds with antimicrobial activity (e.g.phenazines) (Berg et al., 2001, Berg, 2009) and hydrolytic enzymes catalyzing cell wall lysis, they will control growth and activity of pathogenic fungi (Krechel et al., 2002).”
From Intensify production, transform biomass to energy and novel goods and protect soils in Europe—A vision how to mobilize marginal lands (2018) · cites this paper as Berg et al., 2001
Cites
Biological Control of Soilborne Plant Pathogens in the Rhizosphere with Bacteria
Weller, D. M. (1988) · Annual Review of Phytopathology · doi:10.1146/annurev.py.26.090188.002115 · Cited by 1,765 · see citing works
Smalla, K., Wieland, G., Buchner, A., Zock, A., Parzy, J., Kaiser, S., Roskot, N., Heuer, H., & Berg, G. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.10.4742-4751.2001 · Cited by 1,185 · see citing works
Self-citationBiocontrol of plant disease: a (Gram-) positive perspective
Emmert, E. A. B., & Handelsman, J. (1999) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1999.tb13405.x · Cited by 633 · see citing works
Suppression of Take-All of Wheat by Seed Treatments with Fluorescent Pseudomonads
Weller, D. M., & Cook, R. J. (2008) · Phytopathology · doi:10.1094/phyto-73-463 · Cited by 531 · see citing works
Compendium of Strawberry Diseases
Mans, J. L. (1985) · Mycologia · doi:10.2307/3793273 · Cited by 369 · see citing works
What makes Pseudomonas bacteria rhizosphere competent?
Lugtenberg, B. J. J., & Dekkers, L. C. (1999) · Environmental Microbiology · doi:10.1046/j.1462-2920.1999.00005.x · Cited by 339 · see citing works
Plant growth stimulation by inoculation with symbiotic and associative rhizosphere microorganisms
Höflich, G., Wiehe, W., & Kühn, G. (1994) · Cellular and Molecular Life Sciences · doi:10.1007/bf01923476 · Cited by 196 · see citing works
The rhizosphere as a habitat for soil microorganisms.
Sørensen, J. C., van Elsas, J. D., Trevors, J. T., & Wellington, E. M. H. (1997) · Research at the University of Copenhagen (University of Copenhagen) · Cited by 192 · see citing works
Nielsen, P., & Sørensen, J. (2006) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.1997.tb00370.x · Cited by 177 · see citing works
Biological Control Of Soilborne Plant Pathogens In The Rhizosphere With Bacteria
Weller, D. M. (1988) · Annual Review of Phytopathology · doi:10.1146/annurev.phyto.26.1.379 · Cited by 168 · see citing works
Biological Control of Fungal Strawberry Diseases by Serratia plymuthica HRO-C48
Kurze, S., Bahl, H., Dahl, R. A., et al. (2001) · Plant Disease · doi:10.1094/pdis.2001.85.5.529 · Cited by 126 · see citing works
Mechanisms of biocontrol of soil-borne plant pathogens by Rhizobacteria
Chet, I., Ordentlich, A., Shapira, R., et al. (1991) · Springer eBooks · doi:10.1007/978-94-011-3336-4_49 · Cited by 126 · see citing works
Mechanisms of biocontrol of soil-borne plant pathogens by Rhizobacteria
Chet, I., Ordentlich, A., Shapira, R., et al. (1990) · Plant and Soil · doi:10.1007/bf00011694 · Cited by 121 · see citing works
Isolation of Microbial Antagonists for Biocontrol of Grey Mould Disease of Strawberries
SWADLING, I. R., & Jeffries, P. (1996) · Biocontrol Science and Technology · doi:10.1080/09583159650039584 · Cited by 66 · see citing works
Reddy, M. S., Hynes, R. K., & Lazarovits, G. (1994) · Canadian Journal of Microbiology · doi:10.1139/m94-018 · Cited by 63 · see citing works
Berg, G., Kurze, S., Buchner, A., et al. (2000) · Canadian Journal of Microbiology · doi:10.1139/w00-101 · Cited by 62 · see citing works
Self-citationBiocontrol of plant disease: a (Gram-) positive perspective
Emmert, E. A. B. (1999) · FEMS Microbiology Letters · doi:10.1016/s0378-1097(98)00570-9 · Cited by 61 · see citing works
Whipps, J. M., van Elsas, J. D., Trevors, J. T., et al. (1997) · Cited by 56 · see citing works
Biological control ofVerticillium dahliaekleb. by natural occurring rhizosphere bacteria
Berg, G., Knaape, C., Ballin, G., et al. (1994) · Archives of Phytopathology and Plant Protection · doi:10.1080/03235409409383116 · Cited by 55 · see citing works
Self-citationEffects ofPseudomonas fluorescenson Potato Plant Growth and Control ofVerticillium dahliae
Leben, S. D. (1987) · Phytopathology · doi:10.1094/phyto-77-1592 · Cited by 46 · see citing works
Scholte, K., & s'Jacob, J. J. (1989) · Potato Research · doi:10.1007/bf02357852 · Cited by 28 · see citing works
Nielsen, P. (1997) · FEMS Microbiology Ecology · doi:10.1016/s0168-6496(96)00089-x · Cited by 24 · see citing works
Berg, G., Kurze, S., Buchner, A., et al. (2000) · Canadian Journal of Microbiology · doi:10.1139/cjm-46-12-1128 · Cited by 18 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Krechel, A., Faupel, A., Hallmann, J., Ulrich, A., & Berg, G. (2002) · Canadian Journal of Microbiology · doi:10.1139/w02-071 · Cited by 188 · see citing works
Self-citationSchröder, P., Beckers, B., Daniels, S., Gnädinger, F., Maestri, E., Marmiroli, N., Mench, M., Millan, R., Obermeier, M. M., Oustriere, N., et al. (2018) · Science of The Total Environment · doi:10.1016/j.scitotenv.2017.10.209 · Cited by 151 · see citing works
- Authors
- Berg, G., Fritze, A., Roskot, N., & Smalla, K.
- Affiliations
- University of Rostock (DE)
- ORCID
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-12
- Volume
- 91
- Issue
- 6
- Pages
- 963-971
- ISSN
- 1364-5072 (ISSN-L)
- 1365-2672
- OpenAlex
- W1999412779
- Cited by
- 149
- References
- 23
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 3 · 2023: 8 · 2022: 7 · 2021: 11 · 2020: 7 · 2019: 10 · 2018: 8 · 2017: 6 · 2016: 4 · 2015: 4 · 2014: 5 · 2013: 9 · 2012: 6
- FWCI
- 3.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Disease Management Techniques
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Disease Resistance and Genetics
- Keywords
- Verticillium dahliae, Verticillium wilt, biological control, rhizosphere bacteria, antagonistic bacteria, plant growth promotion, Pseudomonas putida, Pseudomonas chlororaphis, Serratia plymuthica, strawberry, potato, oilseed rape, microplate assay
- MeSH terms
- Antibiosis, Brassica, Gram-Negative Bacteria, Pest Control, Biological, Plant Diseases, Plants, Solanum tuberosum, Pseudomonas, Serratia, Pseudomonas putida, Plant Roots, Verticillium, Plant Development
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
