Potato-associated bacteria and their antagonistic potential towards plant-pathogenic fungi and the plant-parasitic nematode Meloidogyne incognita(Kofoid & White) Chitwood
Abstract
To study the effect of microenvironments on potato-associated bacteria, the abundance and diversity of bacteria isolated from the rhizosphere, phyllosphere, endorhiza, and endosphere of field grown potato was analyzed. Culturable bacteria were obtained after plating on R2A medium. The endophytic populations averaged 10(3) and 10(5) CFU/g (fresh wt.) for the endosphere and endorhiza. respectively, which were lower than those for the ectophytic microenvironments, with 10(5) and 10(7) CFU/g (fresh wt.) for the phyllosphere and rhizosphere, respectively. The composition and richness of bacterial species was microenvironment-dependent. The occurrence and diversity of potato-associated bacteria was additionally monitored by a cultivation-independent approach using terminal restriction fragment length polymorphism analysis of 16S rDNA. The patterns obtained revealed a high heterogeneity of community composition and suggested the existence of microenvironment-specific communities. In an approach to measure the antagonistic potential of potato-associated bacteria, a total of 440 bacteria was screened by dual testing for in vitro antagonism towards the soilborne pathogens Verticillium dahliae and Rhizoctonia solani. The proportion of isolates with antagonistic activity was highest for the rhizosphere (10%), followed by the endorhiza (9%), phyllosphere (6%), and endosphere (5%). All 33 fungal antagonists were characterized by testing their in vitro antagonistic mechanisms, including their glucanolytic, chitinolytic, pectinolytic, cellulolytic, and proteolytic activity, and by their BOX-PCR fingerprints. In addition, they were screened for their biocontrol activity against Meloidogyne incognita. Overall, nine isolates belonging to Pseudomonas and Streptomyces species were found to control both fungal pathogens and M. incognita and were therefore considered as promising biological control agents.
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Microorganisms can produce a wide array of compounds with antimicrobial activity and hydrolytic enzymes catalyzing cell wall lysis, and they will control growth and activity of pathogenic fungi.
“Since microorganisms can produce a wide array of compounds with antimicrobial activity (e.g.phenazines) (Berg et al., 2001, Berg, 2009) and hydrolytic enzymes catalyzing cell wall lysis, they will control growth and activity of pathogenic fungi (Krechel et al., 2002).”
From Intensify production, transform biomass to energy and novel goods and protect soils in Europe—A vision how to mobilize marginal lands (2018) · cites this paper as Krechel et al., 2002
Cites
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302 · see citing works
A Mathematical Theory of Communication
Shannon, C. E. (1948) · Bell System Technical Journal · doi:10.1002/j.1538-7305.1948.tb00917.x · Cited by 49,574 · see citing works
The Mathematical Theory of Communication
Shannon, C. E., Weaver, W., & Wiener, N. (1950) · Physics Today · doi:10.1063/1.3067010 · Cited by 9,875 · see citing works
Two simple media for the demonstration of pyocyanin and fluorescin.
Eo, K., WARD, M. K., & De, R. (1954) · PubMed · Cited by 5,209 · see citing works
A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique
HUSSEY, R. S., & Barker, K. R. (1973) · The Plant disease reporter · Cited by 2,412 · see citing works
Liu, W., Marsh, T. L., Cheng, H. H., & Forney, L. J. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.11.4516-4522.1997 · Cited by 2,252 · see citing works
Bacterial endophytes in agricultural crops
Hallmann, J., Quadt-Hallmann, A., Mahaffee, W. F., & Kloepper, J. W. (1997) · Canadian Journal of Microbiology · doi:10.1139/m97-131 · Cited by 2,018 · see citing works
Self-citationBiological Control of Soilborne Plant Pathogens in the Rhizosphere with Bacteria
Weller, D. M. (1988) · Annual Review of Phytopathology · doi:10.1146/annurev.py.26.090188.002115 · Cited by 1,765 · see citing works
Molecular basis of plant growth promotion and biocontrol by rhizobacteria
Bloemberg, G. V., & Lugtenberg, B. J. J. (2001) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(00)00183-7 · Cited by 1,049 · see citing works
Bacterial Endophytes: Potential Role in Developing Sustainable Systems of Crop Production
Sturz, A. V., Christie, B. R., & Nowak, J. (2000) · Critical Reviews in Plant Sciences · doi:10.1016/s0735-2689(01)80001-0 · Cited by 857 · see citing works
Klement, Z., Rudolph, K. W. E., & Sands, D. C. (1990) · Cited by 750 · see citing works
Biocontrol of plant disease: a (Gram-) positive perspective
Emmert, E. A. B., & Handelsman, J. (1999) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1999.tb13405.x · Cited by 633 · see citing works
A Comparison of Some Species‐Individuals Diversity Indices Applied to Samples of Field Insects
Menhinick, E. F. (1964) · Ecology · doi:10.2307/1934933 · Cited by 573 · see citing works
Role of Antibiosis in the Biocontrol of Plant Diseases*
Fravel, D. R. (1988) · Annual Review of Phytopathology · doi:10.1146/annurev.py.26.090188.000451 · Cited by 523 · see citing works
Bacterial Endophytes: Potential Role in Developing Sustainable Systems of Crop Production
Sturz, A. V., Christie, B. R., & Nowak, J. (2000) · Critical Reviews in Plant Sciences · doi:10.1080/07352680091139169 · Cited by 496 · see citing works
Frequency of Antibiotic-Producing Pseudomonas spp. in Natural Environments
Raaijmakers, J. M., Weller, D. M., & Thomashow, L. S. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.3.881-887.1997 · Cited by 495 · see citing works
Infection and Colonization of Sugar Cane and Other Graminaceous Plants by Endophytic Diazotrophs
James, E. K. (1998) · Critical Reviews in Plant Sciences · doi:10.1016/s0735-2689(98)00357-8 · Cited by 376 · see citing works
Chitinolytic Enterobacter agglomerans Antagonistic to Fungal Plant Pathogens
Chernin, L. S., Ismailov, Z. F., Haran, S., & Chet, I. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.5.1720-1726.1995 · Cited by 375 · see citing works
Determination of bacterially derived auxins using a microplate method
Sarwar, M., & Kremer, R. J. (1995) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.1995.tb00446.x · Cited by 293 · see citing works
Araújo, W. L., Maccheroni, W., Aguilar-Vildoso, C. I., et al. (2001) · Canadian Journal of Microbiology · doi:10.1139/w00-146 · Cited by 291 · see citing works
Garbeva, P., van Overbeek, L. S., Vuurde, J. W. L. V., et al. (2001) · Microbial Ecology · doi:10.1007/s002480000096 · Cited by 252 · see citing works
Raaijmakers, J. M., & Weller, D. M. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.6.2545-2554.2001 · Cited by 247 · see citing works
Frommel, M. I., Nowak, J., & Lazarovits, G. (1991) · PLANT PHYSIOLOGY · doi:10.1104/pp.96.3.928 · Cited by 224 · see citing works
Plant and Nematode Interactions
(1998) · Agronomy monograph/Agronomy · doi:10.2134/agronmonogr36 · Cited by 220 · see citing works
Sturz, A. V., Christie, B. R., & Matheson, B. G. (1998) · Canadian Journal of Microbiology · doi:10.1139/w97-146 · Cited by 183 · see citing works
Bacterial endophytes in cotton: mechanisms of entering the plant
Quadt-Hallmann, A., Kloepper, J. W., & Benhamou, N. (1997) · Canadian Journal of Microbiology · doi:10.1139/m97-081 · Cited by 182 · see citing works
Improving plant productivity with rhizosphere bacteria
Ryder, M., Stephens, P., & Bowen, G. D. (1994) · Cited by 176 · see citing works
Advances in Verticillium: Research and Disease Management
(2001) · Journal of Phytopathology · doi:10.1046/j.1439-0434.2001.0596d.x · Cited by 161 · see citing works
Infection and Colonization of Sugar Cane and Other Graminaceous Plants by Endophytic Diazotrophs
James, E. K., & Olivares, F. L. (1998) · Critical Reviews in Plant Sciences · doi:10.1080/07352689891304195 · Cited by 155 · see citing works
Adhikari, T. B., Joseph, C. M. L., Yang, G., et al. (2001) · Canadian Journal of Microbiology · doi:10.1139/w01-097 · Cited by 153 · see citing works
Plant interactions with endophytic bacteria.
Hallmann, J. (2001) · CABI Publishing eBooks · doi:10.1079/9780851995120.0087 · Cited by 153 · see citing works
Self-citationBerg, G., Fritze, A., Roskot, N., & Smalla, K. (2001) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2001.01462.x · Cited by 149 · see citing works
Self-citationNatural incidence of endophytic bacteria in pea cultivars under field conditions
Elvira‐Recuenco, M., & Vuurde, J. W. L. V. (2000) · Canadian Journal of Microbiology · doi:10.1139/w00-098 · Cited by 147 · see citing works
Bacterial endophytes in cotton: location and interaction with other plant-associated bacteria
Quadt-Hallmann, A., Hallmann, J., & Kloepper, J. W. (1997) · Canadian Journal of Microbiology · doi:10.1139/m97-035 · Cited by 130 · see citing works
Self-citationMechanisms of biocontrol of soil-borne plant pathogens by Rhizobacteria
Chet, I., Ordentlich, A., Shapira, R., et al. (1991) · Springer eBooks · doi:10.1007/978-94-011-3336-4_49 · Cited by 126 · see citing works
Mechanisms of biocontrol of soil-borne plant pathogens by Rhizobacteria
Chet, I., Ordentlich, A., Shapira, R., et al. (1990) · Plant and Soil · doi:10.1007/bf00011694 · Cited by 121 · see citing works
Hasky-Guenther, K., Hoffmann-Hergarten, S., & Sikora, R. A. (1998) · Fundamental & applied nematology · Cited by 112 · see citing works
Effects of Rhizobacteria on Root-Knot Nematodes and Gall Formation
Becker, J. O. (1988) · Phytopathology · doi:10.1094/phyto-78-1466 · Cited by 105 · see citing works
Downing, K. J., & Thomson, J. A. (2000) · Canadian Journal of Microbiology · doi:10.1139/w99-147 · Cited by 102 · see citing works
Berg, G., Kurze, S., Buchner, A., et al. (2000) · Canadian Journal of Microbiology · doi:10.1139/w00-101 · Cited by 62 · see citing works
Self-citationEffect of stunt and root knot nematodes on Verticillium wilt of tomato.
Overman, A. J., & Jones, J. P. (1970) · Phytopathology · Cited by 9 · see citing works
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Exploiting plant–microbe partnerships to improve biomass production and remediation
Weyens, N., van der Lelie, D., Taghavi, S., Newman, L., & Vangronsveld, J. (2009) · Trends in Biotechnology · doi:10.1016/j.tibtech.2009.07.006 · Cited by 592 · see citing works
Schröder, P., Beckers, B., Daniels, S., Gnädinger, F., Maestri, E., Marmiroli, N., Mench, M., Millan, R., Obermeier, M. M., Oustriere, N., et al. (2018) · Science of The Total Environment · doi:10.1016/j.scitotenv.2017.10.209 · Cited by 151 · see citing works
- Authors
- Krechel, A., Faupel, A., Hallmann, J., Ulrich, A., & Berg, G.
- Affiliations
- University of Rostock (DE)
- Venue
- Canadian Journal of Microbiology
- Publisher
- Canadian Science Publishing
- Published
- 2002-09-01
- Volume
- 48
- Issue
- 9
- Pages
- 772-786
- ISSN
- 0008-4166 (ISSN-L)
- 1480-3275
- OpenAlex
- W2090531462
- Cited by
- 188
- References
- 41
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 8 · 2023: 4 · 2022: 5 · 2021: 7 · 2020: 9 · 2019: 10 · 2018: 6 · 2017: 6 · 2016: 16 · 2015: 12 · 2014: 7 · 2013: 15 · 2012: 4
- FWCI
- 7.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Canadian Journal of Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Nematode management and characterization studies
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Keywords
- rhizosphere bacteria, phyllosphere bacteria, bacterial community composition, plant pathogenic fungi, Verticillium dahliae, Rhizoctonia solani, Meloidogyne incognita, biological control, antagonistic bacteria, Pseudomonas, Streptomyces, bacterial diversity, 16S rRNA gene, BOX-PCR fingerprinting, enzyme activity
- MeSH terms
- Animals, Antibiosis, Bacteria, Culture Media, Mitosporic Fungi, DNA, Ribosomal, Fatty Acids, Pest Control, Biological, Plant Diseases, Solanum tuberosum, Polymorphism, Restriction Fragment Length, Rhizoctonia, RNA, Ribosomal, 16S, Soil Microbiology, Tylenchoidea, Ecosystem, Plant Roots, Verticillium
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