The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Pseudomonas aeruginosa GacA, a factor in multihost virulence, is also essential for biofilm formation
Abstract
We have investigated a potential role for GacA, the response regulator of the GacA/GacS two‐component regulatory system, in Pseudomonas aeruginosa biofilm formation. When gacA was disrupted in strain PA14, a 10‐fold reduction in biofilm formation capacity resulted relative to wild‐type PA14. However, no significant difference was observed in the planktonic growth rate of PA14 gacA−. Providing gacA in trans on the multicopy vector pUCP‐gacA abrogated the biofilm formation defect. Scanning electron microscopy of biofilms formed by PA14 gacA− revealed diffuse clusters of cells that failed to aggregate into microcolonies, implying a deficit in biofilm development or surface translocation. Motility assays revealed no decrease in PA14 gacA− twitching or swimming abilities, indicating that the defect in biofilm formation is independent of flagellar‐mediated attachment and solid surface translocation by pili. Autoinducer and alginate bioassays were performed similarly, and no difference in production levels was observed, indicating that this is not merely an upstream effect on either quorum sensing or alginate production. Antibiotic susceptibility profiling demonstrated that PA14 gacA− biofilms have moderately decreased resistance to a range of antibiotics relative to PA14 wild type. This study establishes GacA as a new and independent regulatory element in P. aeruginosa biofilm formation.
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Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies
Pang, Z., Raudonis, R., Glick, B. R., Lin, T.-J., & Cheng, Z. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2018.11.013 · Cited by 2,306
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Biofilm Formation and Dispersal under the Influence of the Global Regulator CsrA of Escherichia coli
Jackson, D. W., Suzuki, K., Oakford, L., Simecka, J. W., Hart, M. E., & Romeo, T. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.1.290-301.2002 · Cited by 425
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Metadata
- Authors
- Parkins, M. D., Ceri, H., & Storey, D. G.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2001-06
- Volume
- 40
- Issue
- 5
- Pages
- 1215-1226
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2105660417
- Cited by
- 263
- References
- 58
- FWCI
- 4.23
- Type
- journal article
- Language
- en
- Open access
- closed
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- Parkins, M. D., Ceri, H., & Storey, D. G. (2001). Pseudomonas aeruginosa GacA, a factor in multihost virulence, is also essential for biofilm formation. Molecular Microbiology. https://doi.org/10.1046/j.1365-2958.2001.02469.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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