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MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake
Open accessAbstract
Summary: The Staphylococcus aureus DtxR‐like protein, MntR, controls expression of the mntABC and mntH genes, which encode putative manganese transporters. Mutation of mntABC produced a growth defect in metal‐depleted medium and increased sensitivity to intracellularly generated superoxide radicals. These phenotypes resulted from diminished uptake of manganese and were rescued by the addition of excess Mn(II). Resistance to superoxide was incompletely rescued by Mn(II) for STE035 ( mntA mntH ), and the strain had reduced virulence in a murine abscess model of infection. Expression of mntABC was repressed by Mn(II) in an MntR‐dependent manner, which contrasts with the expression of mntH that was not repressed in elevated Mn(II) and was decreased in an mntR mutant. This demonstrates that MntR acts as a negative and positive regulator of these loci re‐spectively. PerR, the peroxide resistance regulon repressor, acts with MntR to control the expression of mntABC and manganese uptake. The expression of the PerR‐regulated genes, katA (catalase), ftn (ferritin) and fur (ferric uptake regulator), was diminished in STE031 ( mntR ) when grown in excess Mn(II). Therefore, the control of Mn(II)‐regulated members of the PerR regulon and the Fur protein is modulated by MntR through its control of Mn(II) uptake. The co‐ordinated regulation of metal ion homeostasis and oxidative stress resistance via the regulators MntR, PerR and Fur of S. aureus is discussed.
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In the citing paper
“In addition to regulation by MntR, mntABC is negatively regulated by PerR, a manganese and iron-dependent Fur homologue that controls responses to oxidative stress [144, 145].”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
Experimental — accuracy not yet measuredIn the citing paper
“In contrast, mutation of mntH does not lead to an appreciable growth defect in metal-depleted media”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
In the citing paper
“The S. aureus genome encodes MntABC-type (mntABC) and Nramp-type (mntH) manganese transporters, both of which have been functionally characterized.”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
In the citing paper
“In manganese-replete conditions, transcription of mntABC is repressed by MntR.”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
In the citing paper
“Mutation of mntA in S. aureus results in a reduced growth rate in metal-depleted minimal media, a phenotype that is reversible upon addition of manganese.”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
In the citing paper
“Deletion of mntR leads to constitutive transcription of mntABC irrespective of manganese levels.”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
In the citing paper
“suggesting that MntABC may be the dominant manganese transporter in S. aureus under in vitro conditions [144].”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
In the citing paper
“The transcriptional regulator MntR affects expression of both mntABC and mntH.”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
In the citing paper
“In S. aureus, mutation of mntA, mntH, or mntR in the laboratory strain 8325-4 does not lead to a significant reduction in bacterial recovery in a murine skin abscess model.”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
In the citing paper
“Additionally, S. aureus employs superoxide dismutase-independent mechanisms to detoxify superoxide, a process that is also dependent on manganese [144].”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
In the citing paper
“Disruption of mntA, mntH, mntR, or both mntA and mntH also significantly reduces intracellular survival in human endothelial cells [144].”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
In the citing paper
“In contrast, concurrent inactivation of mntA and mntH significantly decreases bacterial recovery in this model.”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [144]
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Citations
Cites
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
Staphylococcus aureus Infections
Lowy, F. D. (1998) · New England Journal of Medicine · doi:10.1056/nejm199808203390806 · Cited by 6,018
Iron and metal regulation in bacteria
Hantke, K. (2001) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(00)00184-3 · Cited by 755
Transition Metals in Control of Gene Expression
O’Halloran, T. V. (1993) · Science · doi:10.1126/science.8342038 · Cited by 626
Antibiotic-resistance cassettes for Bacillus subtilis
Guérout, A.-M., Shazand, K., Frandsen, N., & Stragier, P. (1995) · Gene · doi:10.1016/0378-1119(95)00652-4 · Cited by 595
[27] Genetic systems in Staphylococci
Novick, R. P. (1991) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(91)04029-n · Cited by 562
Lack of a role for iron in the Lyme disease pathogen
Posey, J. E., & Gherardini, F. C. (2000) · Science · doi:10.1126/science.288.5471.1651 · Cited by 538
Improved method for electroporation of Staphylococcus aureus
Schenk, S., & Laddaga, R. A. (1992) · FEMS Microbiology Letters · doi:10.1016/0378-1097(92)90596-g · Cited by 431
The scavenging of superoxide radical by manganous complexes: In vitro
Archibald, F. S., & Fridovich, I. (1982) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(82)90049-2 · Cited by 427
Iron uptake mechanisms of pathogenic bacteria
Wooldridge, K. G., & WILLIAMS, P. H. (1993) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.1993.tb00026.x · Cited by 426
Chakraborty, T., Leimeister-Wächter, M., Domann, E., Hartl, M., Goebel, W., Nichterlein, T., & Notermans, S. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.2.568-574.1992 · Cited by 403
Bsat, N., Herbig, A. F., Casillas-Martínez, L., Setlow, P., & Helmann, J. D. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.00921.x · Cited by 378
Manganese and Defenses against Oxygen Toxicity in Lactobacillus plantarum
Archibald, F. S., & Fridovich, I. (1981) · Journal of Bacteriology · doi:10.1128/jb.145.1.442-451.1981 · Cited by 373
Horsburgh, M. J., Clements, M. O., Crossley, H., Ingham, E., & Foster, S. J. (2001) · Infection and Immunity · doi:10.1128/iai.69.6.3744-3754.2001 · Cited by 365
Self-citationHorsburgh, M. J., Ingham, E., & Foster, S. J. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.2.468-475.2001 · Cited by 316
Self-citationKehres, D. G., Zaharik, M. L., Finlay, B. B., & Maguire, M. E. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.01922.x · Cited by 292
Higuchi, R. (1989) · Palgrave Macmillan UK eBooks · doi:10.1007/978-1-349-20235-5_6 · Cited by 290
Que, Q., & Helmann, J. D. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.01811.x · Cited by 282
Out of the iron age: new insights into the critical role of manganese homeostasis in bacteria
Jakubovics, N. S., & Jenkinson, H. F. (2001) · Microbiology · doi:10.1099/00221287-147-7-1709 · Cited by 267
Gold, B., Rodríguez, G. M., Marras, S. A. E., Pentecost, M., & Smith, I. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02684.x · Cited by 262
Molecular biology of the staphylococci
Hamilton, W. A. (1991) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(91)90185-x · Cited by 262
Berry, A. M., & Paton, J. C. (1996) · Infection and Immunity · doi:10.1128/iai.64.12.5255-5262.1996 · Cited by 261
Gaballa, A., & Helmann, J. D. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.22.5815-5821.1998 · Cited by 258
Sullivan, M. A., Yasbin, R. E., & Young, F. E. (1984) · Gene · doi:10.1016/0378-1119(84)90161-6 · Cited by 258
Bearden, S. W., & Perry, R. D. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01360.x · Cited by 254
Makui, H., Roig, E. A., Cole, S. T., Helmann, J. D., Gros, P., & Cellier, M. F. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.01774.x · Cited by 240
Peacock, S. J., Foster, T. J., Cameron, B., & Berendt, A. R. (1999) · Microbiology · doi:10.1099/00221287-145-12-3477 · Cited by 237
Self-citationCoordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen peroxide and metal ions
Chen, L., Keramati, L., & Helmann, J. D. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.18.8190 · Cited by 229
Herbig, A. F., & Helmann, J. D. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02543.x · Cited by 224
Chan, P. F., Foster, S. J., Ingham, E., & Clements, M. O. (1998) · Journal of Bacteriology · doi:10.1128/.180.23.6082-6089.1998 · Cited by 222
Self-citationChan, P. F., Foster, S. J., Ingham, E., & Clements, M. O. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.23.6082-6089.1998 · Cited by 187
Self-citationClements, M. O., Watson, S. P., & Foster, S. J. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.13.3898-3903.1999 · Cited by 185
Self-citationSingh, K. V., Coque, T. M., Weinstock, G. M., & Murray, B. E. (1998) · FEMS Immunology & Medical Microbiology · doi:10.1111/j.1574-695x.1998.tb01180.x · Cited by 179
Massey, R. C., Καντζάνου, Μ., Fowler, T., Day, N. P. J., Schofield, K. M., Wann, E. R., Berendt, A. R., Höök, M., & Peacock, S. J. (2001) · Cellular Microbiology · doi:10.1046/j.1462-5822.2001.00157.x · Cited by 176
Self-citationManganese-dependent disproportionation of hydrogen peroxide in bicarbonate buffer.
Stadtman, E. R., Berlett, B. S., & Chock, P. B. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.1.384 · Cited by 172
A new family of high-affinity ABC manganese and zinc permeases
Claverys, J. (2001) · Research in Microbiology · doi:10.1016/s0923-2508(01)01195-0 · Cited by 169
FimA, a major virulence factor associated with Streptococcus parasanguis endocarditis
Burnette-Curley, D., Wells, V. D., Viscount, H. B., Munro, C. L., Fenno, J. C., Fives-Taylor, P. M., & Macrina, F. L. (1995) · Infection and Immunity · doi:10.1128/iai.63.12.4669-4674.1995 · Cited by 169
Tseng, H., Srikhanta, Y. N., McEwan, A. G., & Jennings, M. P. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02460.x · Cited by 164
Foster, S. J. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.19.5723-5725.1995 · Cited by 163
Self-citationYeşilkaya, H., Kadioglu, A., Gingles, N. A., Alexander, J. E., Mitchell, T. J., & Andrew, P. W. (2000) · Infection and Immunity · doi:10.1128/iai.68.5.2819-2826.2000 · Cited by 162
Patzer, S. I., & Hantke, K. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.16.4806-4813.2001 · Cited by 160
Manganese acquisition by Lactobacillus plantarum
Archibald, F. S., & Duong, M. N. (1984) · Journal of Bacteriology · doi:10.1128/jb.158.1.1-8.1984 · Cited by 158
Manganese: Its Acquisition by and Function in the Lactic Acid Bacteria
Archibald, F. S. (1986) · CRC Critical Reviews in Microbiology · doi:10.3109/10408418609108735 · Cited by 147
Iron, DtxR, and the regulation of diphtheria toxin expression
Tao, X., Schiering, N., Zeng, H., Ringe, D., & Murphy, J. R. (1994) · Molecular Microbiology · doi:10.1111/j.1365-2958.1994.tb01280.x · Cited by 143
Antioxidant effect of manganese
Coassin, M., Ursini, F., & Bindoli, A. (1992) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(92)90282-2 · Cited by 136
Recent advances in the understanding of the biological chemistry of manganese
Yocum, C. F., & Pecoraro, V. L. (1999) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(99)80031-3 · Cited by 133
Kolenbrander, P. E., Andersen, R. N., Baker, R. A., & Jenkinson, H. F. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.2.290-295.1998 · Cited by 124
Inaoka, T., Matsumura, Y., & Tsuchido, T. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.6.1939-1943.1999 · Cited by 121
Horsburgh, M. J., & Moir, A. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01323.x · Cited by 111
Self-citationJakubovics, N. S., Smith, A. W., & Jenkinson, H. F. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.02122.x · Cited by 111
SirR, a Novel Iron-Dependent Repressor in Staphylococcus epidermidis
Hill, P. J., Cockayne, A., Landers, P., Morrissey, J. A., Sims, C. M., & Williams, P. M. (1998) · Infection and Immunity · doi:10.1128/iai.66.9.4123-4129.1998 · Cited by 110
Investigations of the state of the manganese in Lactobacillus plantarum
Archibald, F. S., & Fridovich, I. (1982) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(82)90120-5 · Cited by 109
Schmitt, M. P., & Holmes, R. K. (1994) · Journal of Bacteriology · doi:10.1128/jb.176.4.1141-1149.1994 · Cited by 108
Cadmium and manganese transport in Staphylococcus aureus membrane vesicles
Perry, R. D., & Silver, S. D. (1982) · Journal of Bacteriology · doi:10.1128/jb.150.2.973-976.1982 · Cited by 108
Characterization of a manganese-dependent regulatory protein, TroR, from Treponema pallidum
Posey, J. E., Hardham, J. M., Norris, S. J., & Gherardini, F. C. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.19.10887 · Cited by 105
Genomic organization of the mycobacterial sigma gene cluster
Doukhan, L., Predich, M., Nair, G., Dussurget, O., Mandić-Mulec, I., Cole, S. T., Smith, D. R., & Smith, I. (1995) · Gene · doi:10.1016/0378-1119(95)00427-8 · Cited by 99
Cockayne, A., Hill, P. J., Powell, N. B. L., Bishop, K., Sims, C., & Williams, P. M. (1998) · Infection and Immunity · doi:10.1128/iai.66.8.3767-3774.1998 · Cited by 98
Genetic Studies on Plasmid-Linked Cadmium Resistance in Staphylococcus aureus
Smith, K., & Novick, R. P. (1972) · Journal of Bacteriology · doi:10.1128/jb.112.2.761-772.1972 · Cited by 95
Polyphyletic origins of bacterial Nramp transporters
Cellier, M. F. (2001) · Trends in Genetics · doi:10.1016/s0168-9525(01)02364-2 · Cited by 87
Lindsay, J. A., & Foster, S. J. (2001) · Microbiology · doi:10.1099/00221287-147-5-1259 · Cited by 84
Self-citationValderas, M. W., & Hart, M. E. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.11.3399-3407.2001 · Cited by 77
Characterization of the Primary σ Factor of Staphylococcus aureus
Deora, R., & Misra, T. K. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.36.21828 · Cited by 73
Keen, C. L., Lönnerdal, B., & Hurley, L. S. (1984) · Springer eBooks · doi:10.1007/978-1-4684-4775-0_5 · Cited by 67
Analysis of transcriptional control of the gerD spore germination gene of Bacillus subtilis 168
Kemp, E. H., Sammons, R. L., Moir, A., Sun, D., & Setlow, P. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.15.4646-4652.1991 · Cited by 63
Ezra, F. S., Lucas, D. S., Mustacich, R. V., & Russell, A. F. (1983) · Biochemistry · doi:10.1021/bi00285a020 · Cited by 44
31P-NMR and ESR studies of the oxidation states of manganese in Staphylococcus aureus
Ezra, F. S., Lucas, D. S., & Russell, A. F. (1984) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/0167-4889(84)90059-4 · Cited by 14
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Metadata
- Authors
- Horsburgh, M. J., Wharton, S. J., Cox, A. G., Ingham, E., Peacock, S., & Foster, S. J.
- Affiliations
- University of Sheffield (GB)
- University of Leeds (GB)
- John Radcliffe Hospital (GB)
- Corresponding author
- Foster, Simon J.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2002-05-23
- Volume
- 44
- Issue
- 5
- Pages
- 1269-1286
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2154476329
- Cited by
- 228
- References
- 65
- Citations by year
- 2026: 7 · 2025: 7 · 2024: 7 · 2023: 4 · 2022: 4 · 2021: 8 · 2020: 15 · 2019: 15 · 2018: 11 · 2017: 9 · 2016: 12 · 2015: 13 · 2014: 9 · 2013: 14 · 2012: 8
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- MntR, PerR, Staphylococcus aureus, manganese uptake, MntH, oxidative stress resistance, fur, kata, ferritin, metal homeostasis, gene expression regulation, virulence
- MeSH terms
- Animals, Bacterial Proteins, Base Sequence, Endothelium, Vascular, Genes, Bacterial, Herbicides, Homeostasis, Humans, Manganese, Mice, Inbred BALB C, Molecular Sequence Data, Paraquat, Promoter Regions, Genetic, Recombinant Fusion Proteins, Repressor Proteins, Staphylococcus aureus, Superoxides, Transcription Factors, Gene Expression Regulation, Bacterial, Genes, Reporter, Regulon, Mice
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