The DOI matches its Crossref record.
Genetic and molecular evidence that the Pseudomonas syringae type III effector protein AvrRpt2 is a cysteine protease
Abstract
Summary: Upon delivery to the plant cell during infection, the Pseudomonas syringae effector protein AvrRpt2 undergoes proteolytic processing, enhances pathogen virulence and causes the elimination of the Arabidopsis RIN4 protein. A structure‐prediction method was employed in order to investigate possible biochemical functions of AvrRpt2. Results of a secondary structure prediction algorithm suggest that the functional C‐terminal portion of AvrRpt2 is a cysteine protease. Mutation of predicted catalytic residues within this portion of AvrRpt2 abolished in planta processing, elimination of Arabidopsis RIN4, and the ability to trigger an RPS2‐specific resistance response. These data indicate that AvrRpt2 is most likely a sequence divergent cysteine protease whose activity is required for elimination of RIN4 during infection.
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Citations
Cited by
Jones, J. D. G., & Dangl, J. L. (2006) · Nature · doi:10.1038/nature05286 · Cited by 13,177
Host-Microbe Interactions: Shaping the Evolution of the Plant Immune Response
Chisholm, S. T., Coaker, G., Day, B., & Staskawicz, B. J. (2006) · Cell · doi:10.1016/j.cell.2006.02.008 · Cited by 2,957
Self-citationTwo Pseudomonas syringae Type III Effectors Inhibit RIN4-Regulated Basal Defense in Arabidopsis
Kim, M. G., da Cunha, L., McFall, A. J., Belkhadir, Y., DebRoy, S., Dangl, J. L., & Mackey, D. (2005) · Cell · doi:10.1016/j.cell.2005.03.025 · Cited by 494
RIN4 Functions with Plasma Membrane H+-ATPases to Regulate Stomatal Apertures during Pathogen Attack
Liu, J., Elmore, J. M., Fuglsang, A. T., Palmgren, M. G., Staskawicz, B. J., & Coaker, G. (2009) · PLoS Biology · doi:10.1371/journal.pbio.1000139 · Cited by 275
Self-citationHow plants recognize pathogens and defend themselves
de Wit, P. J. G. M. (2007) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-007-7284-7 · Cited by 263
Metadata
- Authors
- Axtell, M. J., Chisholm, S. T., Dahlbeck, D., & Staskawicz, B. J.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2003-09
- Volume
- 49
- Issue
- 6
- Pages
- 1537-1546
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2109183496
- Cited by
- 229
- References
- 0
- FWCI
- 21.80
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Axtell, M. J., Chisholm, S. T., Dahlbeck, D., & Staskawicz, B. J. (2003). Genetic and molecular evidence that the Pseudomonas syringae type III effector protein AvrRpt2 is a cysteine protease. Molecular Microbiology. https://doi.org/10.1046/j.1365-2958.2003.03666.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
