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dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics
Open accessAbstract
Summary: PCR‐based detection of single nucleotide polymorphisms is a powerful tool for the plant geneticist. Cleaved amplified polymorphic sequence analysis is the most widely used approach for the detection of single nucleotide polymorphisms. However, this technique is limited to mutations which create or disrupt a restriction enzyme recognition site. This paper presents a modification of this technique where mismatches in a PCR primer are used to create a polymorphism based on the target mutation. This technique is useful for following known mutations in segregating populations and genetic mapping of isolated DNAs used for positional based cloning of new genes. In addition, a computer program has been developed that facilitates the design of these PCR primers.
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Citations
Cited by
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Interactions among APETALA1, LEAFY, and TERMINAL FLOWER1 Specify Meristem Fate
Liljegren, S. J., Gustafson-Brown, C., Pinyopich, A., Ditta, G. S., & Yanofsky, M. F. (1999) · The Plant Cell · doi:10.1105/tpc.11.6.1007 · Cited by 423
A QTL for flowering time in Arabidopsis reveals a novel allele of CRY2
El-Din El-Assal, S., Alonso-Blanco, C., Peeters, A. J. M., Raz, V., & Koornneef, M. (2001) · Nature Genetics · doi:10.1038/ng767 · Cited by 398
BIG: a calossin-like protein required for polar auxin transport in Arabidopsis
Gil, P., Dewey, E., Friml, J., Zhao, Y., Snowden, K. C., Putterill, J., Palme, K., Estelle, M., & Chory, J. (2001) · Genes & Development · doi:10.1101/gad.905201 · Cited by 269
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Kim, J. I., Dolan, W. L., Anderson, N. A., & Chapple, C. (2015) · The Plant Cell · doi:10.1105/tpc.15.00127 · Cited by 117
Metadata
- Authors
- Neff, M. M., Neff, J. D., Chory, J., & Pepper, A. E.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 1998-05
- Volume
- 14
- Issue
- 3
- Pages
- 387-392
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2035177738
- Cited by
- 717
- References
- 0
- FWCI
- 8.15
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics. https://app.cochat.ai/papers/10.1046/j.1365-313x.1998.00124.x
- The paper
- Neff, M. M., Neff, J. D., Chory, J., & Pepper, A. E. (1998). dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics. The Plant Journal. https://doi.org/10.1046/j.1365-313x.1998.00124.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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