The CUP-SHAPED COTYLEDON1 gene of Arabidopsis regulates shoot apical meristem formation
Abstract
In higher plants, molecular mechanisms regulating shoot apical meristem (SAM) formation and organ separation are largely unknown. The CUC1 (CUP-SHAPED COTYLEDON1) and CUC2 are functionally redundant genes that are involved in these processes. We cloned the CUC1 gene by a map-based approach, and found that it encodes a NAC-domain protein highly homologous to CUC2. CUC1 mRNA was detected in the presumptive SAM during embryogenesis, and at the boundaries between floral organ primordia. Surprisingly, overexpression of CUC1 was sufficient to induce adventitious shoots on the adaxial surface of cotyledons. Expression analyses in the overexpressor and in loss-of-function mutants suggest that CUC1 acts upstream of the SHOOT MERISTEMLESS gene.
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Transcripts of LOB, CUC1, and CUC2 are detected at the boundary of the SAM and the new leaf primordium.
“Transcripts of all these genes are detected at the boundary of two tissues, the SAM and the new leaf primordium (Aida et al. 1999; Takada et al. 2001; Shuai et al. 2002).”
From Molecular analysis of the <i>LATERAL SUPPRESSOR</i> gene in <i>Arabidopsis</i> reveals a conserved control mechanism for axillary meristem formation (2003) · cites this paper as Takada et al. 2001
Vascular bundles contribute to the induction of meristems.
“The close proximity between the developing vascular elements and developing axillary meristems, combined with the observation that ectopic meristems form preferentially over underlying vascular bundles (Chuck et al. 1996; Takada et al. 2001), suggests that vascular bundles contribute to the induction of meristems.”
From Molecular analysis of the <i>LATERAL SUPPRESSOR</i> gene in <i>Arabidopsis</i> reveals a conserved control mechanism for axillary meristem formation (2003) · cites this paper as Takada et al. 2001
Ectopic meristems form preferentially over underlying vascular bundles.
“the observation that ectopic meristems form preferentially over underlying vascular bundles (Chuck et al. 1996; Takada et al. 2001)”
From Molecular analysis of the <i>LATERAL SUPPRESSOR</i> gene in <i>Arabidopsis</i> reveals a conserved control mechanism for axillary meristem formation (2003) · cites this paper as Takada et al. 2001
The pattern of LAS transcript accumulation in the axils of leaf primordia is similar to those of LOB, CUC1, and CUC2.
“The pattern of LAS transcript accumulation in the axils of leaf primordia is similar to those of the genes LATERAL ORGAN BOUNDARIES ( LOB ), CUPSHAPED COTYLEDON1 ( CUC1 ), and CUP-SHAPED COTYLEDON2 ( CUC2 ).”
From Molecular analysis of the <i>LATERAL SUPPRESSOR</i> gene in <i>Arabidopsis</i> reveals a conserved control mechanism for axillary meristem formation (2003) · cites this paper as Takada et al. 2001
NAM/CUC family genes are consistently expressed in boundary zones and their loss of function causes fusion of adjacent organs that would normally be separated.
“These genes are consistently expressed in boundary zones and their loss of function causes fusion of adjacent organs (e.g.cotyledons, floral organs, and ovules) that would normally be separated (Souer et al., 1996;Aida et al., 1997;Takada et al., 2001;Vroemen et al., 2003;Weir et al., 2004; Fig. 1e,f).”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Takada et al., 2001
The Arabidopsis CUC1, CUC2 and CUC3 genes encode NAC protein family proteins.
“| CUC1-3 | Gob (tomato (Solanum | NAC | Fusions of various organs, | SAM and lateral organs | Souer et al. (1996); Aida et al. |”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Takada et al. (2001)
Loss-of-function mutants of CUC1-3 show fusions of various organs.
“| CUC1-3 | Gob (tomato (Solanum | NAC | Fusions of various organs, | SAM and lateral organs | Souer et al. (1996); Aida et al. |”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Takada et al. (2001)
Cites
Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant.
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Self-citationSouer, E., van Houwelingen, A., Kloos, D., Mol, J., & Koes, R. (1996) · Cell · doi:10.1016/s0092-8674(00)81093-4 · Cited by 1,059 · see citing works
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Greb, T., Clarenz, O., Schäfer, E., Müller, D., Herrero, R., Schmitz, G., & Theres, K. (2003) · Genes & Development · doi:10.1101/gad.260703 · Cited by 541 · see citing works
The NAC-domain transcription factor GOBLET specifies leaflet boundaries in compound tomato leaves
Berger, Y., Harpaz-Saad, S., Brand, A., Melnik, H., Sirding, N., Alvarez, J. P., Zinder, M., Samach, A., Eshed, Y., & Ori, N. (2009) · Development · doi:10.1242/dev.031625 · Cited by 305 · see citing works
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<i>CUPULIFORMIS</i> establishes lateral organ boundaries in <i>Antirrhinum</i>
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<i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems
Wang, Q., Hasson, A., Rossmann, S., & Theres, K. (2016) · New Phytologist · doi:10.1111/nph.13641 · Cited by 106 · see citing works
- Authors
- Takada, S., Hibara, K.-I., Ishida, T., & Tasaka, M.
- Affiliations
- Kyoto University (JP)
- Corresponding author
- Tasaka, Masao
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2001-04-01
- Volume
- 128
- Issue
- 7
- Pages
- 1127-1135
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2136594478
- Cited by
- 587
- References
- 2
- Citations by year
- 2026: 16 · 2025: 14 · 2024: 10 · 2023: 19 · 2022: 38 · 2021: 22 · 2020: 26 · 2019: 18 · 2018: 25 · 2017: 33 · 2016: 13 · 2015: 27 · 2014: 23 · 2013: 28 · 2012: 31
- FWCI
- 22.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Keywords
- CUC1, CUC2, floral organ primordia, adventitious shoot induction, SHOOT MERISTEMLESS, Arabidopsis thaliana, gene redundancy, positional cloning
- MeSH terms
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Molecular Sequence Data, Plant Proteins, Mutagenesis, Genes, Plant, Arabidopsis, Sequence Homology, Amino Acid, Meristem, DNA, Plant, Gene Expression Profiling, Arabidopsis Proteins
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