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Application of high-performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids
Open accessAbstract
The application of high-performance liquid chromatography (HPLC) using a C30 reverse-phase stationary matrix has enabled the simultaneous separation of carotenes, xanthophylls, ubiquinones, tocopherols and plastoquinones in a single chromatogram. Continuous photodiode array (PDA) detection ensured identification and quantification of compounds upon elution. Applications of the method to the characterization of transgenic and mutant tomato varieties with altered isoprenoid content, biochemical screening of Arabidopsis thaliana, and elucidation of the modes of action of bleaching herbicides are described to illustrate the versatility of the procedure.
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Rohmer, M., & Rohmer, M. (1999) · Natural Product Reports · doi:10.1039/a709175c · Cited by 1,193
Cited by
Metabolomics—the link between genotypes and phenotypes
Fiehn, O. (2002) · Plant Molecular Biology · doi:10.1023/a:1013713905833 · Cited by 3,445
Simkin, A. J., Schwartz, S. H., Auldridge, M., Taylor, M. G., & Klee, H. J. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2004.02263.x · Cited by 474
Toledo-Ortiz, G., Huq, E., & Rodríguez-Concepción, M. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914428107 · Cited by 453
Unintended effects and their detection in genetically modified crops
Cellini, F., Chesson, A., Colquhoun, I., Constable, A., Davies, H. V., Engel, K. H., Gatehouse, A. M. R., Kärenlampi, S., Kok, E. J., Leguay, J.-J., et al. (2004) · Food and Chemical Toxicology · doi:10.1016/j.fct.2004.02.003 · Cited by 397
Hasunuma, T., Kikuyama, F., Matsuda, M., Aikawa, S., Izumi, Y., & Kondo, A. (2013) · Journal of Experimental Botany · doi:10.1093/jxb/ert134 · Cited by 160
“both chlorophyll a and carotenoids (Fraser et al., 2000), the decrease in the absorbance at 430 nm indicated a decrease in these pigments.”
Metadata
- Authors
- Fraser, P. D., Pinto, M. E. S., Holloway, D. E., & Bramley, P. M.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2000-11
- Volume
- 24
- Issue
- 4
- Pages
- 551-558
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W1965916795
- Cited by
- 410
- References
- 1
- FWCI
- 3.49
- Type
- journal article
- Language
- en
- Open access
- bronze
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- The paper
- Fraser, P. D., Pinto, M. E. S., Holloway, D. E., & Bramley, P. M. (2000). Application of high-performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids. The Plant Journal. https://doi.org/10.1046/j.1365-313x.2000.00896.x
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
