Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion
Open accessAbstract
Cyanobacteria represent a globally important biomass because they are responsible for a substantial proportion of primary production in the hydrosphere. Arthrospira platensis is a fast-growing halophilic cyanobacterium capable of accumulating glycogen and has the potential to serve as a feedstock in the fermentative production of third-generation biofuels. Accordingly, enhancing cyanobacterial glycogen production is a promising biofuel production strategy. However, the regulatory mechanism of glycogen metabolism in cyanobacteria is poorly understood. The aim of the present study was to determine the metabolic flux of glycogen biosynthesis using a dynamic metabolomic approach. Time-course profiling of widely targeted cyanobacterial metabolic intermediates demonstrated a global metabolic reprogramming that involves transient increases in the levels of some amino acids during the glycogen production phase induced by nitrate depletion. Also, in vivo labelling with NaH(13)CO3 enabled direct measurement of metabolic intermediate turnover in A. platensis, revealing that under conditions of nitrate depletion glycogen is biosynthesized with carbon derived from amino acids released from proteins via gluconeogenesis. This dynamic metabolic profiling approach provided conclusive evidence of temporal alterations in the metabolic profile in cyanobacterial cells.
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In presence of nitrate, glycogen and protein contents of Arthrospira remained nearly constant at ~15% DW and ~50% DW, respectively.
“In the presence of nitrate, the glycogen and protein contents remained nearly constant at ~15% DW and ~50% DW, respectively.”
From Arthrospira platensis as a Feasible Feedstock for Bioethanol Production (2021) · cites this paper as [64]
Time-course profiling demonstrated transient increases in levels of other amino acids (glycine, histidine, isoleucine, leucine, methionine, phenylalanine, proline, threonine, tyrosine).
“Time-course profiling of metabolites demonstrated transient increases in the levels of other amino acids (glycine, histidine, isoleucine, leucine, methionine, phenylalanine, proline, threonine, and tyrosine).”
From Arthrospira platensis as a Feasible Feedstock for Bioethanol Production (2021) · cites this paper as [64]
Shifting cyanobacterial cells into nitrogen depletion and cultivating for 72 h resulted in global metabolic modifications involving reductions in free amino acids (aspartate, glutamate, glutamine) and increases in organic acids (2-ketoglutarate, malate, succinate).
“Analyzing the metabolic intermediates, they observed that shifting the cyanobacterial cells into nitrogen depletion and cultivating them for 72 h resulted in global metabolic modifications that involved reductions in the levels of several free amino acids (aspartate, glutamate, and glutamine), and increased in the levels of organic acids (2-ketoglutarate, malate, and succinate).”
From Arthrospira platensis as a Feasible Feedstock for Bioethanol Production (2021) · cites this paper as [64]
In absence of nitrate, glycogen content increased to 63.2% DW while protein content decreased to 15.4%.
“In contrast, in the absence of nitrate, the glycogen content increased to 63.2% DW while the protein content decreased to 15.4%.”
From Arthrospira platensis as a Feasible Feedstock for Bioethanol Production (2021) · cites this paper as [64]
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- Authors
- Hasunuma, T., Kikuyama, F., Matsuda, M., Aikawa, S., Izumi, Y., & Kondo, A.
- Affiliations
- Japan Science and Technology Agency (JP)
- Kobe University (JP)
- Corresponding author
- Hasunuma, Tomohisa
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-05-08
- Volume
- 64
- Issue
- 10
- Pages
- 2943-2954
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- PMC
- PMC3697948
- OpenAlex
- W2103644949
- Cited by
- 160
- References
- 45
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 10 · 2023: 8 · 2022: 16 · 2021: 18 · 2020: 10 · 2019: 15 · 2018: 12 · 2017: 12 · 2016: 17 · 2015: 13 · 2014: 17 · 2013: 1
- FWCI
- 7.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Journal of Experimental Botany (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Japan Science and Technology Agency
- Precursory Research for Embryonic Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- Spirulina platensis, nitrate depletion, metabolic flux, metabolic reprogramming, amino acid accumulation, gluconeogenesis, metabolic intermediates, third-generation biofuels, halophilic cyanobacteria
- MeSH terms
- Cyanobacteria, Amino Acids, Gluconeogenesis, Glycogen, Nitrates, Metabolome, Biofuels
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