Characterization of a defensin in bark and fruit tissues of peach and antimicrobial activity of a recombinant defensin in the yeast, Pichia pastoris
Abstract
Herein the cloning and characterization of a defensin gene (PpDfn1) from a cDNA library made from peach (Prunus persica[L.] Batsch) winter bark tissues is described. A partial clone obtained from the library was extended to full length by 5′ Rapid Amplification of cDNA Ends (RACE). The open reading frame of 237 bp codes for a 79 amino acid peptide related to the defensin family of proteins. Sequence comparison of the encoded protein using blast analysis revealed significant homology to defensins from other plant species. RNA gel blot analysis indicated that the gene is seasonally expressed in bark tissues of 1‐year‐old shoots, and is also expressed in early fruit development. Results of quantitative RT‐PCR and protein blot analysis were similar to those of RNA gel blot analyses for the bark tissues. A recombinant version, rDFN1 was expressed in the yeast, Pichia pastoris. It was found that rDFN1 inhibited germination of the fungal pathogens Penicillium expansum and Botrytis cinerea, but not the Gram‐negative bacterium Erwinia amylovora. The potential physiological role of PpDFN1 and its antimicrobial properties are discussed.
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A P. pastoris strain was engineered for improved control of P. expansum by expressing the recombinant peach and pea defensins rDFN1 or rPsd1.
“A P. pastoris strain was also engineered for improved control of P. expansum by expressing the recombinant peach and pea defensins rDFN1 or rPsd1, respectively (Janisiewicz et al. 2008; Wisniewski et al. 2003).”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Wisniewski et al. 2003
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- Authors
- Wisniewski, M. E., Bassett, C. L., Artlip, T. S., Webb, R. P., Janisiewicz, W. J., Norelli, J. L., Goldway, M., & Droby, S.
- Affiliations
- Appalachian Fruit Research Laboratory (US)
- Migal - Galilee Technology Center (IL)
- Agricultural Research Organization (IL)
- Corresponding author
- Wisniewski, Michael E.
- ORCID
- Venue
- Physiologia Plantarum
- Publisher
- Wiley
- Published
- 2003-11-12
- Volume
- 119
- Issue
- 4
- Pages
- 563-572
- ISSN
- 0031-9317 (ISSN-L)
- 1399-3054
- OpenAlex
- W2133832625
- Cited by
- 37
- References
- 42
- Citations by year
- 2022: 1 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 3 · 2015: 3 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 1.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Physiologia Plantarum (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Postharvest Quality and Shelf Life Management
- Keywords
- defensins, peach, bark tissue, fruit development, seasonal gene expression, Pichia pastoris, antimicrobial activity, Penicillium expansum, Botrytis cinerea, Erwinia amylovora, fungal pathogen inhibition, plant defense peptides
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