The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Electrophysiological properties of microglial cells in normal and pathologic rat brain slices
Abstract
UNLABELLED: Microglial cells serve as pathologic sensors of the brain. They are highly abundant in all regions of the central nervous system (CNS) and are characterized by a ramified morphology within the normal tissue. In the present study, we have developed a procedure to study the membrane properties of identified, in situ microglia in acutely isolated brain slices from rat cortex, striatum and facial nucleus. Unlike the well characterized cultured microglial cells, ramified microglia of the slice are characterized by little, if any, voltage-gated membrane currents and a very low membrane potential. They are thus distinct from neurons, other glial cells and nonbrain macrophages. To study the consequences of microglial activation on the membrane channel pattern, we compared cells in the normal facial nucleus and at defined times after facial nerve axotomy. Within 12 h of axotomy, microglial cells expressed a prominent inward rectifier current and thus acquired the physiological properties of cultured microglia. Within 24 h of the lesion, the cells expressed an additional outward current, which is typical for lipopolysaccharide (LPS)-activated microglia in vitro. Seven days after the lesion, at a time of major regenerative processes in the facial nucleus, the physiological properties of microglial cells had reverted to those present prior to the pathological event. IN CONCLUSION: (i) ramified microglial cells represent a physiologically unique population of cells in the brain; (ii) are distinct from their cultured counterparts; and (iii), undergo a defined pattern of physiological states in the course of pathologic events.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Development of outward K+ current in microglia is associated with an advanced microglial activation status (Boucsein et al., 2000; Wendt et al., 2017)”
From Overexpressing low‐density lipoprotein receptor reduces tau‐associated neurodegeneration in relation to apoE‐linked mechanisms (2021) · cites this paper as Boucsein et al., 2000
In the citing paper
“VTA and SNc microglia exhibited resting potentials similar to those reported for cortical microglia (Boucsein et al., 2000; Boucsein et al., 2003)”
From Local cues establish and maintain region-specific phenotypes of basal ganglia microglia (2017) · cites this paper as Boucsein et al., 2000
In the citing paper
“microglia filled with biocytin during recording exhibited ramified morphology comparable to that reported previously (Boucsein et al., 2000; Boucsein et al., 2003)”
From Local cues establish and maintain region-specific phenotypes of basal ganglia microglia (2017) · cites this paper as Boucsein et al., 2000
Citations
Cites
Hamill, O. P., Marty, A., Neher, E., Sakmann, B., & Sigworth, F. J. (1981) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/bf00656997 · Cited by 18,436
Microglia: a sensor for pathological events in the CNS
Kreutzberg, G. W. (1996) · Trends in Neurosciences · doi:10.1016/0166-2236(96)10049-7 · Cited by 4,396
Gerdes, J., Lemke, H., Baisch, H., Wacker, H. H., Schwab, U., & Stein, H. (1984) · The Journal of Immunology · doi:10.4049/jimmunol.133.4.1710 · Cited by 4,173
Heterogeneity in the distribution and morphology of microglia in the normal adult mouse brain
Lawson, L. J., Perry, V. H., Dri, P., & Gordon, S. (1990) · Neuroscience · doi:10.1016/0306-4522(90)90229-w · Cited by 2,045
Characterization of ameboid microglia isolated from developing mammalian brain
Giulian, D., & Baker, T. J. (1986) · Journal of Neuroscience · doi:10.1523/jneurosci.06-08-02163.1986 · Cited by 1,300
Raivich, G., Bohatschek, M., Kloss, C. U. A., Werner, A., Jones, L. L., & Kreutzberg, G. W. (1999) · Brain Research Reviews · doi:10.1016/s0165-0173(99)00007-7 · Cited by 843
Potassium and Calcium Channels in Lymphocytes
Lewis, R. S., & Cahalan, M. D. (1995) · Annual Review of Immunology · doi:10.1146/annurev.iy.13.040195.003203 · Cited by 475
Response of endogenous glial cells to motor neuron degeneration induced by toxic ricin
Streit, W. J., & Kreutzberg, G. W. (1988) · The Journal of Comparative Neurology · doi:10.1002/cne.902680209 · Cited by 387
Streit, W. J. (1990) · Journal of Histochemistry & Cytochemistry · doi:10.1177/38.11.2212623 · Cited by 372
Graeber, M. B., Streit, W. J., & Kreutzberg, G. W. (1988) · Journal of Neuroscience Research · doi:10.1002/jnr.490210104 · Cited by 367
Molecular mechanisms of microglial activation
Zielasek, J., & Hartung, H. (1996) · Advances in Neuroimmunology · doi:10.1016/0960-5428(96)00017-4 · Cited by 249
Kettenmann, H., Hoppe, D., Gottmann, K., Banati, R. B., & Kreutzberg, G. W. (1990) · Journal of Neuroscience Research · doi:10.1002/jnr.490260303 · Cited by 229
Self-citationIon channels in microglia (brain macrophages)
Eder, C. (1998) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1998.275.2.c327 · Cited by 196
Acarín, L., Vela, J. M., González, B., & Castellano, B. (1994) · Journal of Histochemistry & Cytochemistry · doi:10.1177/42.8.8027523 · Cited by 195
Electrophysiological behavior of microglia
Kettenmann, H., Banati, R. B., & Walz, W. (1993) · Glia · doi:10.1002/glia.440070115 · Cited by 166
Self-citationExpression of NR1 and NR2A/B subunits of the NMDA receptor in cortical astrocytes
Conti, F., Debiasi, S., Minelli, A., & Melone, M. (1996) · Glia · doi:10.1002/(sici)1098-1136(199607)17:3 · Cited by 143
Amigorena, S., Choquet, D., Teillaud, J., Korn, H., & Fridman, W. H. (1990) · The Journal of Immunology · doi:10.4049/jimmunol.144.6.2038 · Cited by 109
Voltage‐dependent potassium channels in activated rat microglia.
Nörenberg, W., Gebicke-Haerter, P. J., & Illes, P. (1994) · The Journal of Physiology · doi:10.1113/jphysiol.1994.sp020046 · Cited by 107
Inflammatory stimuli induce a new K+ outward current in cultured rat microglia
Nörenberg, W., Gebicke-Haerter, P. J., & Illéš, P. (1992) · Neuroscience Letters · doi:10.1016/0304-3940(92)90587-w · Cited by 106
ATP-induced membrane currents in ameboid microglia acutely isolated from mouse brain slices
Haas, S. A., Brockhaus, J., Verkhratsky, A., & Kettenmann, H. (1996) · Neuroscience · doi:10.1016/0306-4522(96)00270-9 · Cited by 101
Self-citationK + Channels and the Intracellular Calcium Signal in Human Melanoma Cell Proliferation
Lepple-Wienhues, A., Berweck, S., Böhmig, M., Leo, C. P., Meyling, B., Garbe, C., & Wiederholt, M. (1996) · The Journal of Membrane Biology · doi:10.1007/s002329900066 · Cited by 94
Schmidtmayer, J., Jacobsen, C. B., Miksch, G., & Sievers, J. (1994) · Glia · doi:10.1002/glia.440120403 · Cited by 86
Illés, P., Nörenberg, W., & Gebicke-Haerter, P. J. (1996) · Neurochemistry International · doi:10.1016/0197-0186(95)00133-6 · Cited by 77
Cytokine-dependent K+ channel profile of microglia at immunologically defined functional states
Fischer, H., Eder, C., Hadding, U., & Heinemann, U. (1995) · Neuroscience · doi:10.1016/0306-4522(94)00398-o · Cited by 76
Brockhaus, J., Ilschner, S., Banati, R. B., & Kettenmann, H. (1993) · Journal of Neuroscience · doi:10.1523/jneurosci.13-10-04412.1993 · Cited by 75
Self-citationIon channels in rat microglia and their different sensitivity to lipopolysaccharide and interferon‐γ
Visentin, S., Agresti, C., Patrizio, M., & Levi, G. (1995) · Journal of Neuroscience Research · doi:10.1002/jnr.490420402 · Cited by 70
Draheim, H. J., Prinz, M., Weber, J. R., Weiser, T. W., Kettenmann, H., & Hanisch, U. (1999) · Neuroscience · doi:10.1016/s0306-4522(98)00407-2 · Cited by 63
Self-citationImmunophenotypic characterization of rat brain macrophages in culture
Graeber, M. B., Banati, R. B., Streit, W. J., & Kreutzberg, G. W. (1989) · Neuroscience Letters · doi:10.1016/0304-3940(89)90106-7 · Cited by 58
Increased potassium, chloride, and taurine conductances in astrocytes during hypoosmotic swelling
Olson, J. E., & Li, G. (1997) · Glia · doi:10.1002/(sici)1098-1136(199707)20:3 · Cited by 46
Eder, C., Fischer, H., Hadding, U., & Heinemann, U. (1995) · The Journal of Membrane Biology · doi:10.1007/bf00233542 · Cited by 43
Eder, C., Klee, R., & Heinemann, U. (1997) · Neuroscience Letters · doi:10.1016/s0304-3940(97)00281-4 · Cited by 26
Proton modulation of outward K+ currents in interferon-γ-activated microglia
Eder, C., & Heinemann, U. (1996) · Neuroscience Letters · doi:10.1016/s0304-3940(96)12433-2 · Cited by 14
Lessons from microglia in special sites.
Lawson, L. J., Matyszak, M., & Perry, V. H. (1993) · PubMed · Cited by 10
Klegeris (1997) · doi:10.1002/(sici)1097-4547(19970715)49:2
Research works printed but not linked: 1
- Illes P., 1996, Molecular mechanisms of microglial activation. B. Voltage‐ and purinoceptor‐operated channels in microglia, Neurochem. Int., 29, 13
Cited by
Sorted by citations
Kettenmann, H., Hanisch, U.-K., Noda, M., & Verkhratsky, A. (2011) · Physiological Reviews · doi:10.1152/physrev.00011.2010 · Cited by 3,547
Self-citationMicroglia as a source and target of cytokines
Hanisch, U. (2002) · Glia · doi:10.1002/glia.10161 · Cited by 1,645
TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function
Zhao, Y., Wu, X., Li, X., Jiang, L.-L., Gui, X., Liu, Y., Sun, Y., Zhu, B., Piña-Crespo, J. C., Zhang, M., et al. (2018) · Neuron · doi:10.1016/j.neuron.2018.01.031 · Cited by 763
Neurotransmitter receptors on microglia
Pocock, J. M., & Kettenmann, H. (2007) · Trends in Neurosciences · doi:10.1016/j.tins.2007.07.007 · Cited by 650
Self-citationEyo, U. B., Peng, J., Swiatkowski, P., Mukherjee, A., Bispo, A., & Wu, L.-J. (2014) · Journal of Neuroscience · doi:10.1523/jneurosci.0416-14.2014 · Cited by 509
Metadata
- Authors
- Boucsein, C., Kettenmann, H., & Nolte, C.
- Affiliations
- Max Delbrück Center (DE)
- ORCID
- Kettenmann, Helmut
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2000-06
- Volume
- 12
- Issue
- 6
- Pages
- 2049-2058
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W1992967280
- Cited by
- 166
- References
- 34
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 4 · 2023: 7 · 2022: 6 · 2021: 13 · 2020: 7 · 2019: 8 · 2018: 8 · 2017: 10 · 2016: 5 · 2015: 5 · 2014: 10 · 2013: 4 · 2012: 8
- FWCI
- 5.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Physiology (medical) › Pain Mechanisms and Treatments
- Keywords
- microglia, voltage-gated currents, inward rectifier potassium current, lipopolysaccharide stimulation, facial nerve axotomy, facial nucleus, microglial activation, rat brain slices, cortical microglia, membrane potential
- MeSH terms
- Animals, Animals, Newborn, Cells, Cultured, Cerebral Cortex, Facial Nerve, Female, Lipopolysaccharides, Membrane Potentials, Potassium Channels, Rats, Wistar, Microglia, Patch-Clamp Techniques, Axotomy, Lectins, Plant Lectins, Cell Size, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
