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Cited by 203Adenosine and Purinergic SignalingNeuroinflammation and Neurodegeneration MechanismsPain Mechanisms and Treatments

The DOI matches its Crossref record; 43 of the 44 references it cites were found in a registry or an index.

Purinergic receptors on microglial cells: functional expression in acute brain slices and modulation of microglial activation in vitro

Boucsein, Clemens, Zacharias, Robert, Färber, Katrin, Pavlovic, Sanja, Hanisch, Uwe‐Karsten, Kettenmann, Helmut·2003·European Journal of Neuroscience
DOI 10.1046/j.1460-9568.2003.02663.x
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Abstract

Microglial cells are the pathologic sensors in the brain. ATP released from damaged cells is a candidate for signalling neural injury to microglia. Moreover, ATP is an extracellular messenger for propagating astrocyte activity in the form of Ca2+ waves. To test for the functional expression of purinoreceptors in microglial cells we employed the patch-clamp technique in acute slices of adult mouse brain. ATP triggered a nonselective cationic and a K+ current. Pharmacological screening with purinergic ligands indicated the presence of P2Y1 and P2Y2/4 receptors linked to the activation of a K+ current and P2X receptors, including P2X7, linked to the activation of a nonselective cationic current. These findings suggest that microglial cells in situ express different purinergic receptors with distinct sensitivity and functional coupling. To test for the involvement of purinoreceptors in microglial activation, we stimulated cultured microglial cells with lipopolysaccharide and measured the release of tumour necrosis factor alpha, interleukin-6, interleukin-12 and macrophage inflammatory protein 1alpha, induction of K+ outward currents and nitric oxide release. All these parameters were reduced in the presence of purinergic ligands, indicating that purinergic receptor activation attenuated indicators of microglial activation.

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Research works printed but not linked: 2

  1. Hanisch U.K., 2001, Microglia in the Degenerating and Regenerating CNS, 79
  2. Illes P., 1996, Molecular mechanisms of microglial activation. B. Voltage‐ and purinoceptor‐operated channels in microglia, Neurochem. Int., 29, 13

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Metadata

Authors
Boucsein, C., Zacharias, R., Färber, K., Pavlovic, S., Hanisch, U., & Kettenmann, H.
Affiliations
Max Delbrück Center (DE)
Corresponding author
Kettenmann, Helmut
ORCID
Kettenmann, Helmut
Venue
European Journal of Neuroscience
Publisher
Wiley
Published
2003-06
Volume
17
Issue
11
Pages
2267-2276
ISSN
  • 0953-816X (ISSN-L)
  • 1460-9568
DOI
10.1046/j.1460-9568.2003.02663.x
OpenAlex
W2074389121
Cited by
203
References
44
Citations by year
2026: 1 · 2025: 3 · 2024: 4 · 2023: 8 · 2022: 3 · 2021: 4 · 2020: 10 · 2019: 9 · 2018: 7 · 2017: 8 · 2016: 10 · 2015: 7 · 2014: 11 · 2013: 7 · 2012: 18
FWCI
6.77
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 17 d)
Locations
  • European Journal of Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Physiology (medical) › Pain Mechanisms and Treatments
Keywords
microglial cells, purinergic receptors, ATP signalling, P2Y receptors, P2X7 receptor, microglial activation, potassium currents, lipopolysaccharide, neuroinflammation, nitric oxide, TNF-α, interleukin-6, IL-12, macrophage inflammatory protein-1alpha, acute brain slices
MeSH terms
Adenosine Triphosphate, Animals, Animals, Newborn, Brain, Calcium, Cell Survival, Cells, Cultured, Dose-Response Relationship, Drug, Drug Interactions, Lipopolysaccharides, Magnesium, Membrane Potentials, Mice, Inbred C57BL, Neural Conduction, Nitric Oxide, Receptors, Purinergic, Time Factors, Uridine Diphosphate, Uridine Triphosphate, Vasodilator Agents, Potassium Channels, Microglia, Chloride Channels, Patch-Clamp Techniques, Adenosine-5'-(N-ethylcarboxamide), Mice, In Vitro Techniques

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