The DOI matches its Crossref record; 43 of the 44 references it cites were found in a registry or an index.
Purinergic receptors on microglial cells: functional expression in acute brain slices and modulation of microglial activation in vitro
Abstract
Microglial cells are the pathologic sensors in the brain. ATP released from damaged cells is a candidate for signalling neural injury to microglia. Moreover, ATP is an extracellular messenger for propagating astrocyte activity in the form of Ca2+ waves. To test for the functional expression of purinoreceptors in microglial cells we employed the patch-clamp technique in acute slices of adult mouse brain. ATP triggered a nonselective cationic and a K+ current. Pharmacological screening with purinergic ligands indicated the presence of P2Y1 and P2Y2/4 receptors linked to the activation of a K+ current and P2X receptors, including P2X7, linked to the activation of a nonselective cationic current. These findings suggest that microglial cells in situ express different purinergic receptors with distinct sensitivity and functional coupling. To test for the involvement of purinoreceptors in microglial activation, we stimulated cultured microglial cells with lipopolysaccharide and measured the release of tumour necrosis factor alpha, interleukin-6, interleukin-12 and macrophage inflammatory protein 1alpha, induction of K+ outward currents and nitric oxide release. All these parameters were reduced in the presence of purinergic ligands, indicating that purinergic receptor activation attenuated indicators of microglial activation.
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“microglia filled with biocytin during recording exhibited ramified morphology comparable to that reported previously (Boucsein et al., 2000; Boucsein et al., 2003)”
From Local cues establish and maintain region-specific phenotypes of basal ganglia microglia (2017) · cites this paper as Boucsein et al., 2003
In the citing paper
“VTA and SNc microglia exhibited resting potentials similar to those reported for cortical microglia (Boucsein et al., 2000; Boucsein et al., 2003)”
From Local cues establish and maintain region-specific phenotypes of basal ganglia microglia (2017) · cites this paper as Boucsein et al., 2003
Citations
Cites
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Receptors for Purines and Pyrimidines
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Activated microglia mediate neuronal cell injury via a nitric oxide mechanism
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ATP Released from Astrocytes Mediates Glial Calcium Waves
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Extracellular ATP Triggers Tumor Necrosis Factor‐α Release from Rat Microglia
Hide, I., Tanaka, M., Inoue, A., Nakajima, K., Kohsaka, S., Inoue, K., & Nakata, Y. (2000) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2000.0750965.x · Cited by 447
Research works printed but not linked: 2
- Hanisch U.K., 2001, Microglia in the Degenerating and Regenerating CNS, 79
- Illes P., 1996, Molecular mechanisms of microglial activation. B. Voltage‐ and purinoceptor‐operated channels in microglia, Neurochem. Int., 29, 13
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Hristovska, I. (2019) · theses.fr (ABES) · doi:10.70675/af1c5105z3e0dz43ffzb906z1553e7a23a6e
Zhang, B., Nan, J., Zhang, Y., Zhang, B., Ke, C., Wang, W., Wu, T., Xia, J., Yang, F., He, M., et al. (2026) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2026.119360
Metadata
- Authors
- Boucsein, C., Zacharias, R., Färber, K., Pavlovic, S., Hanisch, U., & Kettenmann, H.
- Affiliations
- Max Delbrück Center (DE)
- Corresponding author
- Kettenmann, Helmut
- ORCID
- Kettenmann, Helmut
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2003-06
- Volume
- 17
- Issue
- 11
- Pages
- 2267-2276
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W2074389121
- Cited by
- 203
- References
- 44
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 8 · 2022: 3 · 2021: 4 · 2020: 10 · 2019: 9 · 2018: 7 · 2017: 8 · 2016: 10 · 2015: 7 · 2014: 11 · 2013: 7 · 2012: 18
- FWCI
- 6.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Physiology (medical) › Pain Mechanisms and Treatments
- Keywords
- microglial cells, purinergic receptors, ATP signalling, P2Y receptors, P2X7 receptor, microglial activation, potassium currents, lipopolysaccharide, neuroinflammation, nitric oxide, TNF-α, interleukin-6, IL-12, macrophage inflammatory protein-1alpha, acute brain slices
- MeSH terms
- Adenosine Triphosphate, Animals, Animals, Newborn, Brain, Calcium, Cell Survival, Cells, Cultured, Dose-Response Relationship, Drug, Drug Interactions, Lipopolysaccharides, Magnesium, Membrane Potentials, Mice, Inbred C57BL, Neural Conduction, Nitric Oxide, Receptors, Purinergic, Time Factors, Uridine Diphosphate, Uridine Triphosphate, Vasodilator Agents, Potassium Channels, Microglia, Chloride Channels, Patch-Clamp Techniques, Adenosine-5'-(N-ethylcarboxamide), Mice, In Vitro Techniques
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