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Cited by 105Evolution and Genetic DynamicsBacterial Genetics and BiotechnologyMicrobial Metabolic Engineering and Bioproduction

The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.

The generation of multiple co‐existing mal ‐regulatory mutations through polygenic evolution in glucose‐limited populations of Escherichia coli

Notley‐McRobb, Lucinda, Ferenci, Thomas·1999·Environmental Microbiology
DOI 10.1046/j.1462-2920.1999.00003.x
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Abstract

The multicomponent glucose transport system of Escherichia coli was used to study the polygenic basis of increased fitness in prolonged nutrient‐limited, continuous cultures. After 280 generations of glucose‐limited growth, nearly all bacteria in four independent chemostat populations exhibited increased glucose transport and contained multiple, stable mutations. Fitter bacteria increased outer membrane permeability for glucose through overexpression of the LamB glycoporin. Three classes of mutation influenced LamB levels as well as regulation of other mal genes. Low‐level mal/lamB constitutivity resulted from mlc mutations acquired in all populations as well as changes at another uncharacterized locus. Larger increases in transporter content resulted from widespread acquisition of a regulatory malT–con mutation in fit isolates. The malT mutations sequenced from 67 adapted isolates were all single base substitutions resulting in amino acid replacements in the N‐terminal third of the MalT activator protein. Analysis of malT–con sequences revealed a mutational spectrum distinct from that found in plate‐selected malT mutants, suggesting that mutational pathways were affected by environmental factors. A second major finding was the remarkable allele diversity in malT within a population derived from a single clone, with at least 11 different alleles co‐existing in a population. The multiplicity of alleles (as well as those found in adaptive mgl changes in the accompanying study) suggest that the periodic selection events observed previously in such populations are not a major factor in reducing genetic diversity. A simple model is presented for the generation of genetic heterogeneity in bacterial populations undergoing polygenic selection.

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Crossref · 10.1046/j.1462-2920.1999.00003.x

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44 of 44 references verified0 citation errors · 0 not checkable

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  • During ALE, clonal interference, bet hedging, genetic hitchhiking and fluctuating growth environments can lead to significant population heterogeneity.

    “Stable phenotypes will accumulate rapidly, although clonal interference [42], bet hedging [43,60], genetic hitchhiking [61] and fluctuating growth environments can lead to significant population heterogeneity [22,62,63].”

    From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [43]

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Research works printed but not linked: 1

  1. Schwartz M.(1987)The maltose regulon. In Escherichia coliandSalmonella typhimurium:Cellular and Molecular Biology. Neidhardt F.C. Ingraham J.L. Low K.B. Magasanik B. Schaechter M. and Umbarger H.E. (eds). Washington DC: American Society for Microbiology Press pp.1482–1502.

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Metadata

Authors
Notley‐McRobb, L., & Ferenci, T.
Affiliations
The University of Sydney (AU)
Corresponding author
Ferenci, Thomas
ORCID
Ferenci, Thomas
Venue
Environmental Microbiology
Publisher
Wiley
Published
1999-02
Volume
1
Issue
1
Pages
45-52
ISSN
  • 1462-2912 (ISSN-L)
  • 1462-2920
DOI
10.1046/j.1462-2920.1999.00003.x
OpenAlex
W2089307705
Cited by
105
References
44
Citations by year
2026: 1 · 2025: 1 · 2021: 7 · 2020: 3 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 4 · 2014: 5 · 2013: 7 · 2012: 6
FWCI
3.49
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 1175 d)
Locations
  • Environmental Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
glucose transport, Escherichia coli, mutation spectrum, allele diversity, genetic heterogeneity, outer membrane permeability
MeSH terms
Adaptation, Physiological, Alleles, Bacterial Outer Membrane Proteins, Bacterial Proteins, Biological Transport, Active, Culture Media, DNA-Binding Proteins, Escherichia coli, Biological Evolution, Genes, Bacterial, Glucose, Mutation, Receptors, Virus, Selection, Genetic, Transcription Factors, Genetic Variation, Porins, Amino Acid Substitution, Escherichia coli Proteins

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