The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Increased DNA Oxidation and Decreased Levels of Repair Products in Alzheimer's Disease Ventricular CSF
Open accessAbstract
Abstract : One of the leading etiologic hypotheses regarding Alzheimer's disease (AD) is the involvement of free radical‐mediated oxidative stress in neuronal degeneration. Although several recent studies show an increase in levels of brain DNA oxidation in both aging and AD, there have been no studies of levels of markers of DNA oxidation in ventricular CSF. This is a study of levels of 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG), the predominant marker of oxidative DNA damage, in intact DNA and as the “free” repair product that results from repair mechanisms. Free 8‐OHdG was isolated from CSF from nine AD and five age‐matched control subjects using solidphase extraction columns and measured using gas chromatography/mass spectrometry with selective ion monitoring. Intact DNA was isolated from the same samples and the levels of 8‐OHdG determined in the intact structures. Quantification of results was carried out using stable isotope‐labeled 8‐OHdG. By using this sensitive methodology, statistically significant elevations (p < 0.05) of 8‐OHdG were observed in intact DNA in AD subjects compared with age‐matched control subjects. In contrast, levels of free 8‐OHdG, removed via repair mechanisms, were depleted significantly in AD samples (p < 0.05). Our results demonstrate an increase in unrepaired oxygen radical‐mediated damage in AD DNA as evidenced by the increased presence of 8‐OHdG in intact DNA and decreased concentrations of the free repair product. These data suggest that the brain in AD may be subject to the double insult of increased oxidative stress, as well as deficiencies in repair mechanisms responsible for removal of oxidized bases.
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Mirra, S. S., Heyman, A., McKeel, D., Sumi, S. M., Crain, B. J., Brownlee, L. M., Vogel, F. S., Hughes, J. P., Belle, G. V., Berg, L., et al. (1991) · Neurology · doi:10.1212/wnl.41.4.479 · Cited by 4,961
Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG
Shibutani, S., Takeshita, M., & Grollman, A. P. (1991) · Nature · doi:10.1038/349431a0 · Cited by 2,224
Excess brain protein oxidation and enzyme dysfunction in normal aging and in Alzheimer disease.
Smith, C. D., Carney, J. M., Starke-Reed, P. E., Oliver, C. N., Stadtman, E. R., Floyd, R. A., & Markesbery, W. R. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.23.10540 · Cited by 1,296
Self-citationOxidative damage to mitochondrial DNA is increased in Alzheimer’s disease
Mecocci, P., MacGarvey, U., & Beal, M. F. (1994) · Annals of Neurology · doi:10.1002/ana.410360510 · Cited by 1,037
Hensley, K., Hall, N., Subramaniam, R., Cole, P., Harris, M., Aksenov, M., Aksenova, M., Gabbita, S. P., Wu, J. F., Carney, J. M., et al. (1995) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1995.65052146.x · Cited by 779 · cited 1×
“, 1995), protein oxidation (Smith et al., 1991; Hensley et al., 1995), 4-hydroxynonenal (HNE), a marker of lipid peroxidation, in AD brain (Markesbery and Lovell, 1998) and ventricular CSF (Lovell et al.”
BackgroundSelf-citationOxidative damage to mitochondrial DNA shows marked age-dependent increases in human brain
Mecocci, P., MacGarvey, U., Kaufman, A. E., Koontz, D., Shoffner, J. M., Wallace, D. C., & Beal, M. F. (1993) · Annals of Neurology · doi:10.1002/ana.410340416 · Cited by 757
Markesbery, W. R., & Lovell, M. A. (1998) · Neurobiology of Aging · doi:10.1016/s0197-4580(98)00009-8 · Cited by 680
Self-citationLyras, L., Cairns, N. J., Jenner, A., Jenner, P., & Halliwell, B. (1997) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1997.68052061.x · Cited by 531 · cited 1×
“Markers of oxidative stress in nuclear and mitochondrial DNA are increased in AD brain (Mecocci et al., 1994; Lyras et al., 1997; Gabbita et al., 1998) and in normal aging (Loft and Poulsen, 1996).”
BackgroundGood, P. F., Perl, D. P., Bierer, L. M., & Schmeidler, J. (1992) · Annals of Neurology · doi:10.1002/ana.410310310 · Cited by 476
Cited by
Ageing as a risk factor for neurodegenerative disease
Hou, Y., Dan, X., Babbar, M., Wei, Y., Hasselbalch, S. G., Croteau, D. L., & Bohr, V. A. (2019) · Nature Reviews Neurology · doi:10.1038/s41582-019-0244-7 · Cited by 3,310
Oxidative DNA damage: mechanisms, mutation, and disease
Cooke, M. S., Evans, M. D., Dizdaroglu, M., & Lunec, J. (2003) · The FASEB Journal · doi:10.1096/fj.02-0752rev · Cited by 3,245
Oxidative Stress in Neurodegenerative Diseases
Simonian, N. A., & Coyle, J. T. (1996) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.36.040196.000503 · Cited by 1,377
Oxidative DNA damage and disease: induction, repair and significance
Evans, M. D., Dizdaroglu, M., & Cooke, M. S. (2004) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2003.11.001 · Cited by 1,372
Oxidative Stress in Neurodegenerative Diseases
Niedzielska, E., Smaga, I., Gawlik, M., Moniczewski, A., Stankowicz, P., Pera, J., & Filip, M. (2016) · Molecular Neurobiology · doi:10.1007/s12035-015-9337-5 · Cited by 800
Metadata
- Authors
- Lovell, M. A., Gabbita, S. P., & Markesbery, W. R.
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 1999-02
- Volume
- 72
- Issue
- 2
- Pages
- 771-776
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W2056585943
- Cited by
- 291
- References
- 9
- FWCI
- 6.05
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Lovell, M. A., Gabbita, S. P., & Markesbery, W. R. (1999). Increased DNA Oxidation and Decreased Levels of Repair Products in Alzheimer's Disease Ventricular CSF. Journal of Neurochemistry. https://doi.org/10.1046/j.1471-4159.1999.0720771.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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