The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Microglia/macrophage‐specific protein Iba1 binds to fimbrin and enhances its actin‐bundling activity
Abstract
Ionized calcium binding adaptor molecule 1 (Iba1) is a microglia/macrophage‐specific calcium‐binding protein. Iba1 has the actin‐bundling activity and participates in membrane ruffling and phagocytosis in activated microglia. In order to understand the Iba1‐related intracellular signalling pathway in greater detail, we employed a yeast two‐hybrid screen to isolate an Iba1‐interacting molecule and identified another actin‐bundling protein, L‐fimbrin. In response to stimulation, L‐fimbrin accumulated and co‐localized with Iba1 in membrane ruffles induced by M‐CSF‐stimulation and phagocytic cups formed by IgG‐opsonized beads in microglial cell line MG5. L‐fimbrin was shown to associate with Iba1 in cell lysate of COS‐7 expressing L‐fimbrin and Iba1. By using purified proteins, direct binding of Iba1 to L‐fimbrin was demonstrated by immunoprecipitation, glutathione S‐transferase pull‐down assays and ligand overlay assays. The binding of Iba1 was also found to increase the actin‐bundling activity of L‐fimbrin. These results indicate that Iba1 forms complexes with L‐fimbrin in membrane ruffles and phagocytic cups, and suggest that Iba1 co‐operates with L‐fimbrin in modulating actin reorganization to facilitate cell migration and phagocytosis by microglia.
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Cites
Microglia-specific localisation of a novel calcium binding protein, Iba1
Ito, D., Imai, Y., Ohsawa, K., Nakajima, K., Fukuuchi, Y., & Kohsaka, S. (1998) · Molecular Brain Research · doi:10.1016/s0169-328x(98)00040-0 · Cited by 1,476
Self-citationImai, Y., Ibata, I., Ito, D., Ohsawa, K., & Kohsaka, S. (1996) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1996.1112 · Cited by 872 · cited 6×
“Although Iba1 possesses actinbundling activity, the amino acid sequence of Iba1 displays no homology to the CH domain (Imai et al. 1996; Sasaki et al. 2001).”
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Cited by
Ramesh, G., MacLean, A. G., & Philipp, M. T. (2013) · Mediators of Inflammation · doi:10.1155/2013/480739 · Cited by 639
Schilling, M., Besselmann, M., Müller, M., Strecker, J. K., Ringelstein, E. B., & Kiefer, R. (2005) · Experimental Neurology · doi:10.1016/j.expneurol.2005.08.004 · Cited by 288
Ahmed, Z., Shaw, G., Sharma, V. P., Yang, C., McGowan, E., & Dickson, D. W. (2007) · Journal of Histochemistry & Cytochemistry · doi:10.1369/jhc.6a7156.2007 · Cited by 258
Microglial immunophenotype in dementia with Alzheimer’s pathology
CFAS, M., Minett, T., Classey, J., Matthews, F. E., Fahrenhold, M., Taga, M., Brayne, C., Ince, P. G., Nicoll, J. A. R., & Boche, D. (2016) · Journal of Neuroinflammation · doi:10.1186/s12974-016-0601-z · Cited by 219
Molecular Mechanisms of Microglial Motility: Changes in Ageing and Alzheimer’s Disease
Franco-Bocanegra, D. K., McAuley, C., Nicoll, J. A. R., & Boche, D. (2019) · Cells · doi:10.3390/cells8060639 · Cited by 147
Metadata
- Authors
- Ohsawa, K., Imai, Y., Sasaki, Y., & Kohsaka, S.
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 2004-01-14
- Volume
- 88
- Issue
- 4
- Pages
- 844-856
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W1593213689
- Cited by
- 368
- References
- 2
- FWCI
- 3.16
- Type
- journal article
- Language
- en
- Open access
- closed
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