Cited by 258Neuroinflammation and Neurodegeneration MechanismsNeuroscience and Neuropharmacology ResearchMedical and Biological Sciences

Actin-binding Proteins Coronin-1a and IBA-1 are Effective Microglial Markers for Immunohistochemistry

Ahmed, Zeshan, Shaw, Gerry, Sharma, Ved P., Yang, Cui, McGowan, Eileen, Dickson, Dennis W.·2007·Journal of Histochemistry & Cytochemistry

Abstract

This study identifies the actin-binding protein, coronin-1a, as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-1a effectively immunostained microglia in human, monkey, horse, rat, and mouse tissues, even in tissues stored for long periods of time. The identity of coronin-1a-immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphological features and the distribution of immunoreactive cells. These properties are shared by another actin-binding protein, IBA-1. Unlike IBA-1, coronin-1a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia.

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Crossref · 10.1369/jhc.6a7156.2007

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Authors
Ahmed, Z., Shaw, G., Sharma, V. P., Yang, C., McGowan, E., & Dickson, D. W.
Affiliations
  • Mayo Clinic (US)
  • University of Florida Health (US)
  • University of Florida (US)
Corresponding author
Dickson, Dennis W.
Venue
Journal of Histochemistry & Cytochemistry
Published
2007-07
Volume
55
Issue
7
Pages
687-700
ISSN
  • 0022-1554 (ISSN-L)
  • 1551-5044
OpenAlex
W2095733296
Cited by
258
References
53
Citations by year
2026: 3 · 2025: 13 · 2024: 7 · 2023: 19 · 2022: 16 · 2021: 21 · 2020: 26 · 2019: 16 · 2018: 18 · 2017: 17 · 2016: 22 · 2015: 12 · 2014: 12 · 2013: 13 · 2012: 17
FWCI
4.45
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Histochemistry & Cytochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Mayo Clinic
  • National Institutes of Health (5r01ag020216-04)
  • National Institute on Aging (P50 AG25711, P50‐AG16574, R01AG022595, R01-AG20216, R01-AG22595, P01-AG-17216, P01AG03949, P01AG003949, P50AG025711, P01AG017216, P50AG016574)
  • National Institute of Neurological Disorders and Stroke (P50-NS40256, P50NS040256)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Health Sciences › Medicine › Anatomy › Medical and Biological Sciences
Keywords
CORO1A, Iba1, microglia, actin-binding proteins, immunohistochemical markers, paraffin-embedded tissue, formalin fixation, lymphocytes, hematopoietic cells, membrane cytoskeleton
MeSH terms
Actins, Animals, Calcium-Binding Proteins, Dogs, Horses, Humans, Immunohistochemistry, Macaca mulatta, Mice, Transgenic, Microfilament Proteins, Neurons, Species Specificity, Biomarkers, Microglia, Coculture Techniques, HL-60 Cells, Mice, Rats

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