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A simple artificial urine for the growth of urinary pathogens
Abstract
A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10(8) cfu ml-1, as found in normal urine. Reproducible, steady-state growth also occurred over many generations in continuous culture. AUM was capable of forming crystals and encrustations resembling those found in natural urinary tract infections. The medium is a suitable replacement for normal urine for use in a wide range of experiments modelling the growth and attachment of urinary pathogens in the clinical environment.
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Cited by
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Marcos, J., Renau, N., Casals, G., Segura, J., Ventura, R., & Pozo, O. J. (2014) · Analytica Chimica Acta · doi:10.1016/j.aca.2013.12.030 · Cited by 76
Stratigou, I.-C., Tsiasioti, A., Tzanavaras, P. D., Markopoulou, C. K., Fytianos, K., & Zacharis, C. K. (2020) · Microchemical Journal · doi:10.1016/j.microc.2020.105260 · Cited by 32
Metadata
- Authors
- Brooks, T., & Keevil, C. W.
- Venue
- Letters in Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1997-03
- Volume
- 24
- Issue
- 3
- Pages
- 203-206
- ISSN
- 0266-8254 (ISSN-L)
- 1472-765X
- OpenAlex
- W2020354602
- Cited by
- 528
- References
- 0
- FWCI
- 0.59
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.