Investigation of endogenous corticosteroids profiles in human urine based on liquid chromatography tandem mass spectrometry
Abstract
The accurate and precise measurement of endogenous corticosteroids in urine is a powerful tool to understand the biochemical state in several diseases. In this study, a rapid, accurate, and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the quantification of 67 endogenous gluco- and mineralo-corticosteroids and progestins has been developed and validated. Sample preparation, chromatographic separation, and mass spectrometric detection were optimized. Urine samples (0.5 mL) were hydrolyzed with β-glucuronidase and the released analytes were extracted by liquid-liquid extraction. The chromatographic separation was performed in 20 min after redisolution of the extract. MS behavior of endogenous corticosteroids was evaluated in order to select the most specific precursor ion ([M+H](+), [M+NH4](+), or [M+H-nH2O](+)) for the detection. MS/MS determination was performed under selected reaction monitoring mode using electrospray ionization in positive mode. The method was shown to be linear (r>0.99) in the range of endogenous concentrations for all studied metabolites. Limits of detection (LOD) below 1 ng mL(-1) were typically obtained for analytes with a 3-oxo-4-ene structure whereas LODs below 15 ng mL(-1) were common for the rest of analytes. Recoveries were higher than 80% and intra-assay precisions below 20%, evaluated at three concentration levels, were found for most steroids. No significant or moderate matrix effect, ranging from 54 to 155%, was observed for most of the analytes. The applicability of the method was confirmed by analyzing 24h urine samples collected from twenty healthy volunteers and comparing the results with previously established normal ranges. The wide coverage of corticosteroid metabolism, together with short analysis time, low sample volume, simple sample preparation, and satisfactory quantitative results make this method useful for clinical purposes.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Two studies report unconjugated excretion of cortisol (30%), cortisone, 20αDHE, 20βDHE, 20βDHF (40-60%); and 20αDHF, 6β-OH-cortisol, 6β-OH-E and 18-OH-cortisol (80-100%).
“Two studies report unconjugated excretion of the following individual steroids: cortisol (30%), cortisone, 20αDHE, 20βDHE, 20βDHF (40–60%); 20αDHF, 6β-OH-cortisol, 6β-OH-E and 18-OH-cortisol (80–100%) [273,274].”
From Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes: a comprehensive review (2019) · cites this paper as [274]
Cites
The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders
Miller, W. L., & Auchus, R. J. (2011) · Endocrine Reviews · doi:10.1210/er.2010-0013 · Cited by 2,458 · see citing works
Allostasis and Allostatic Load Implications for Neuropsychopharmacology
McEwen, B. (2000) · Neuropsychopharmacology · doi:10.1016/s0893-133x(99)00129-3 · Cited by 1,833 · see citing works
Enzymology of human cytosolic sulfotransferases
Falany, C. N. (1997) · The FASEB Journal · doi:10.1096/fasebj.11.4.9068609 · Cited by 604 · see citing works
A simple artificial urine for the growth of urinary pathogens
Brooks, T., & Keevil, C. W. (1997) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.1997.00378.x · Cited by 544 · see citing works
Krone, N., Hughes, B. A., Lavery, G. G., Stewart, P. M., Arlt, W., & Shackleton, C. H. L. (2010) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2010.04.010 · Cited by 434 · see citing works
McCarver, D. G., & Hines, R. N. (2002) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.300.2.361 · Cited by 367 · see citing works
Metabolic disturbances connecting obesity and depression
Hryhorczuk, C., Sharma, S., & Fulton, S. E. (2013) · Frontiers in Neuroscience · doi:10.3389/fnins.2013.00177 · Cited by 322 · see citing works
Profiling steroid hormones and urinary steroids
Shackleton, C. H. L. (1986) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(00)80683-0 · Cited by 291 · see citing works
Oxytocin-Motivated Ally Selection is Moderated by Fetal Testosterone Exposure and Empathic Concern
Kret, M. E., & Dreu, C. K. W. D. (2013) · Frontiers in Neuroscience · doi:10.3389/fnins.2013.00001 · Cited by 288 · see citing works
Steroid Hormone Analysis by Tandem Mass Spectrometry
Soldin, S. J., & Soldin, O. P. (2009) · Clinical Chemistry · doi:10.1373/clinchem.2007.100008 · Cited by 264 · see citing works
Mass spectrometry in the diagnosis of steroid-related disorders and in hypertension research
Shackleton, C. H. L. (1993) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(93)90132-g · Cited by 247 · see citing works
Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories
Kushnir, M. M., Rockwood, A. L., Roberts, W. L., Yue, B., Bergquist, J., & Meikle, A. W. (2010) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2010.07.008 · Cited by 226 · see citing works
The Encyclopedia of Mass Spectrometry
(2016) · Elsevier eBooks · doi:10.1016/c2009-0-05659-1 · Cited by 195 · see citing works
Liquid chromatography–tandem mass spectrometry applications in endocrinology
Kushnir, M. M., Rockwood, A. L., & Bergquist, J. (2009) · Mass Spectrometry Reviews · doi:10.1002/mas.20264 · Cited by 159 · see citing works
Caulfield, M. P., Lynn, T., Gottschalk, M. E., Jones, K. L., Taylor, N. F., Malunowicz, E. M., Shackleton, C. H. L., Reitz, R. E., & Fisher, D. A. (2002) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.87.8.8712 · Cited by 120 · see citing works
Pozo, Ó. J., Eenoo, P. V., Deventer, K., & Delbeke, F. T. (2007) · Journal of Mass Spectrometry · doi:10.1002/jms.1182 · Cited by 96 · see citing works
Pozo, Ó. J., Eenoo, P. V., Deventer, K., Grimalt, S., Sancho, J. V., Hernández, F. J., & Delbeke, F. T. (2008) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.3823 · Cited by 95 · see citing works
Cho, H.-J., Kim, J.-D., Lee, W., Chung, B. C., & Choi, M. H. (2008) · Analytica Chimica Acta · doi:10.1016/j.aca.2008.10.059 · Cited by 86 · see citing works
Deventer, K., & Delbeke, F. T. (2003) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.1157 · Cited by 72 · see citing works
Steroid profile for urine: reference values.
Weykamp, C. W., Penders, T. J., Schmidt, N., Borburgh, A. J., van de Calseyde, J. F., & Wolthers, B. J. (1989) · Clinical Chemistry · doi:10.1093/clinchem/35.12.2281 · Cited by 70 · see citing works
Mazzarino, M., Turi, S., & Botrè, F. (2008) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-007-1802-1 · Cited by 66 · see citing works
Pozo, Ó. J., Ventura, R., Monfort, N., Segura, J., & Delbeke, F. T. (2009) · Journal of Mass Spectrometry · doi:10.1002/jms.1568 · Cited by 49 · see citing works
Yamashita, K., Nakagawa, R., Okuyama, M., Honma, S., Takahashi, M., & Numazawa, M. (2008) · Steroids · doi:10.1016/j.steroids.2008.02.009 · Cited by 41 · see citing works
Urinary profile of methylprednisolone and its metabolites after oral and topical administrations
Matabosch, X., Pozo, Ó. J., Monfort, N., Pérez‐Mañá, C., Farré, M., Marcos, J., Segura, J., & Ventura, R. (2013) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2013.05.019 · Cited by 41 · see citing works
Fabregat, A., Kotronoulas, A., Marcos, J., Joglar, J., Alfonso, I., Segura, J., Ventura, R., & Pozo, O. J. (2013) · Steroids · doi:10.1016/j.steroids.2012.11.017 · Cited by 40 · see citing works
Taylor, N. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-616-0_17 · Cited by 40 · see citing works
Identification of budesonide metabolites in human urine after oral administration
Matabosch, X., Pozo, Ó. J., Pérez‐Mañá, C., Farré, M., Marcos, J., Segura, J., & Ventura, R. (2012) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-012-6037-0 · Cited by 39 · see citing works
Christakoudi, S., Cowan, D., & Taylor, N. (2009) · Steroids · doi:10.1016/j.steroids.2009.09.011 · Cited by 34 · see citing works
The identification of novel steroid N-acetylglucosaminides in the urine of pregnant women
Meng, L. J., Griffiths, W. J., & Sjövall, J. (1996) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(96)00080-5 · Cited by 32 · see citing works
Circadian rhythm of urinary steroid metabolites
Jerjes, W. K., Cleare, A. J., Peters, T. J., & Taylor, N. (2006) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1258/000456306777695564 · Cited by 27 · see citing works
Pozo, Ó. J., Deventer, K., Eenoo, P. V., & Delbeke, F. T. (2007) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.3140 · Cited by 27 · see citing works
Christakoudi, S., Cowan, D., & Taylor, N. (2011) · Steroids · doi:10.1016/j.steroids.2011.12.018 · Cited by 23 · see citing works
Taylor, N. F. (2006) · Humana Press eBooks · doi:10.1385/1-59259-986-9:159 · Cited by 16 · see citing works
Christakoudi, S., Cowan, D., & Taylor, N. (2013) · Steroids · doi:10.1016/j.steroids.2013.02.008 · Cited by 14 · see citing works
Flagged first, then by citations
1 of the 35 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Schiffer, L., Barnard, L., Baranowski, E. S., Gilligan, L. C., Taylor, A. E., Arlt, W., Shackleton, C. H. L., & Storbeck, K.-H. (2019) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2019.105439 · Cited by 450 · see citing works
“OH-E and 18-OH-cortisol (80–100%) [273,274].”
LC–MS/MS analysis of steroids in the clinical laboratory
Keevil, B. G. (2016) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2016.04.009 · Cited by 149 · see citing works
Steuer, A. E., Brockbals, L., & Kræmer, T. (2019) · Frontiers in Chemistry · doi:10.3389/fchem.2019.00319 · Cited by 125 · see citing works
Determination and occurrence of natural and synthetic glucocorticoids in surface waters
Shen, X., Chang, H., Sun, Y., & Wan, Y. (2019) · Environment International · doi:10.1016/j.envint.2019.105278 · Cited by 94 · see citing works
Derivatization of Steroids in Biological Samples for GC–MS and LC–MS Analyses
Marcos, J., & Pozo, Ó. J. (2015) · Bioanalysis · doi:10.4155/bio.15.176 · Cited by 95 · see citing works
Cortisol measurements in Cushing’s syndrome: immunoassay or mass spectrometry?
Casals, G., & Hanzu, F. A. (2020) · Annals of Laboratory Medicine · doi:10.3343/alm.2020.40.4.285 · Cited by 80 · see citing works
Shackleton, C., Pozo, O. J., & Marcos, J. (2018) · Journal of the Endocrine Society · doi:10.1210/js.2018-00135 · Cited by 75 · see citing works
From a single steroid to the steroidome: Trends and analytical challenges
Olesti, E., Boccard, J., Visconti, G., González‐Ruiz, V., & Rudaz, S. L. (2020) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2020.105797 · Cited by 74 · see citing works
Current LC–MS methods and procedures applied to the identification of new steroid metabolites
Marcos, J., & Pozo, Ó. J. (2015) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2015.12.012 · Cited by 58 · see citing works
Hill, M., Hána, V., Velíková, M., Pařı́zek, A., Kolátorová, L., Vítků, J., Škodová, T., Šimková, M., Šimják, P., Kancheva, R. L., et al. (2019) · Physiological Research · doi:10.33549/physiolres.934124 · Cited by 60 · see citing works
Portero-Tresserra, M., Gracia-Rubio, I., Cantacorps, L., Pozo, O. J., Gómez-Gómez, A., Pastor, A., López-Arnau, R., de la Torre, R., & Valverde, O. (2018) · European Neuropsychopharmacology · doi:10.1016/j.euroneuro.2018.02.003 · Cited by 55 · see citing works
Targeting Human Urinary Metabolome by LC–MS/MS: A Review
Rodríguez-Morató, J., Pozo, Ó. J., & Marcos, J. (2018) · Bioanalysis · doi:10.4155/bio-2017-0285 · Cited by 53 · see citing works
Hawley, J. M., & Keevil, B. G. (2016) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2016.05.014 · Cited by 52 · see citing works
Ibáñez, L., del Río, L., Díaz, M., Sebastiani, G., Pozo, Ó. J., López‐Bermejo, A., & de Zegher, F. (2017) · Journal of Adolescent Health · doi:10.1016/j.jadohealth.2017.04.010 · Cited by 51 · see citing works
Gomez-Gomez, A., Miranda, J., Feixas, G., Arranz Betegon, A., Crispi, F., Gratacós, E., & Pozo, O. J. (2020) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2019.105520 · Cited by 50 · see citing works
Progress in nanoparticle-based electrochemical biosensors for hormone detection
Rossi, F., Trakoolwilaiwan, T., Gigli, V., Tortolini, C., Lenzi, A., Isidori, A. M., Thanh, N. T. K., & Antiochia, R. (2024) · Nanoscale · doi:10.1039/d4nr02075h · Cited by 50 · see citing works
Servin-Barthet, C., Martínez‐Garcia, M., Paternina-Die, M., Vidal, L. M., de Blas, D. M., Soler, A., Khymenets, O., Bergé, D., Casals, G., Prats, P., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-55830-0 · Cited by 47 · see citing works
Non-targeted LC-MS based metabolomics analysis of the urinary steroidal profile
Palermo, A., Botrè, F., de la Torre, X., & Zamboni, N. (2017) · Analytica Chimica Acta · doi:10.1016/j.aca.2017.01.055 · Cited by 47 · see citing works
Robles, J., Marcos, J., Renau, N., Garrostas, L., Segura, J., Ventura, R., Martín, B. B., Barceló, A., & Pozo, Ó. J. (2017) · Talanta · doi:10.1016/j.talanta.2017.03.032 · Cited by 44 · see citing works
PACAP-PAC1R modulates fear extinction via the ventromedial hypothalamus
Velasco, E. R., Florido, A., Flores, Á., Senabre, E., Gomez‐Gómez, À., Torres, A., Roca, A., Norrholm, S. D., Newman, E. L., Das, P., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-31442-w · Cited by 44 · see citing works
Sex differences in fear memory consolidation via Tac2 signaling in mice
Florido, A., Velasco, E. R., Soto-Faguás, C. M., Gomez‐Gómez, À., Pérez-Caballero, L., Molina, P., Nadal, R. A., Pozo, Ó. J., Saura, C. A., & Andero, R. (2021) · Nature Communications · doi:10.1038/s41467-021-22911-9 · Cited by 43 · see citing works
Determination of steroid profile in hair by liquid chromatography tandem mass spectrometry
Gomez‐Gómez, À., & Pozo, Ó. J. (2020) · Journal of Chromatography A · doi:10.1016/j.chroma.2020.461179 · Cited by 39 · see citing works
Crovetto, F., Nakaki, A., Arranz, A., Borras, R., Vellvé, K., Paules, C., Boutet, M. L., Castro-Barquero, S., Freitas, T., Casas, R., et al. (2023) · JAMA Network Open · doi:10.1001/jamanetworkopen.2023.30255 · Cited by 41 · see citing works
Metabolomics predicts the pharmacological profile of new psychoactive substances
Olesti, E., Toma, I. D., Ramaekers, J. G., Brunt, T. M., Carbó, M., Fernández-Avilés, C., Robledo, P., Farré, M., Dierssen, M., Pozo, Ó. J., et al. (2018) · Journal of Psychopharmacology · doi:10.1177/0269881118812103 · Cited by 35 · see citing works
Detection and characterization of prednisolone metabolites in human urine by LC‐MS/MS
Matabosch, X., Pozo, Ó. J., Pérez‐Mañá, C., Papaseit, E., Segura, J., & Ventura, R. (2015) · Journal of Mass Spectrometry · doi:10.1002/jms.3571 · Cited by 35 · see citing works
Urinary steroidomic profiles by LC-MS/MS to monitor classic 21-Hydroxylase deficiency
Pussard, E., Travers, S. A. A., Bouvattier, C. M., Xue, Q.-Y., Cosson, C., Viengchareun, S., Martinerie, L., & Lombès, M. (2019) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2019.105553 · Cited by 34 · see citing works
Arioli, F., Gamberini, M. C., Pavlović, R., Cesare, F. D., Draghi, S., Bussei, G., Mungiguerra, F., Casati, A., & Fidani, M. (2022) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-022-04249-3 · Cited by 33 · see citing works
Crovetto, F., Crispi, F., Borràs, R., Paulés, C., Casas, R. M., Martín-Asuero, A., Arranz, Á., Vieta, E., Estruch, R., & Gratacós, E. (2021) · Trials · doi:10.1186/s13063-021-05309-2 · Cited by 32 · see citing works
Thompson, G. R., Beck, K. R., Patt, M., Kratschmar, D. V., & Odermatt, A. (2019) · Journal of the Endocrine Society · doi:10.1210/js.2019-00189 · Cited by 31 · see citing works
Casals, G., Marcos, J., Pozo, Ó. J., Alcaraz, J., de Osaba, M. J. M., & Jiménez, W. (2014) · Journal of Chromatography B · doi:10.1016/j.jchromb.2014.04.015 · Cited by 30 · see citing works
Pozo, Ó. J., Marcos, J., Khymenets, O., Pranata, A., Fitzgerald, C. C. J., McLeod, M. D., & Shackleton, C. H. L. (2018) · Journal of Molecular Endocrinology · doi:10.1530/jme-17-0286 · Cited by 31 · see citing works
Casas, I., Nakaki, A., Pascal, R., Castro‐Barquero, S., Youssef, L., Genero, M., Benítez, L., Larroya, M., Boutet, M. L., Casu, G., et al. (2023) · Nutrients · doi:10.3390/nu15102362 · Cited by 32 · see citing works
Current Challenges and Future Directions in the Assessment of Glucocorticoid Status
Clarke, S. A., Eng, P. C., Comninos, A. N., Lazarus, K., Choudhury, S., Tsang, C., Meeran, K. M., Tan, T., Dhillo, W. S., & Abbara, A. (2024) · Endocrine Reviews · doi:10.1210/endrev/bnae016 · Cited by 30 · see citing works
Xue, Y., He, X., Huan-de, L., Deng, Y., Yan, M., Cai, H., Tang, M., Dang, R.-L., & Jiang, P. (2015) · International Journal of Endocrinology · doi:10.1155/2015/296531 · Cited by 29 · see citing works
Li, M., Wang, H., Huan, X., Cao, N., Guan, H., Zhang, H., Cheng, X. M., & Wang, C. (2021) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-021-03568-1 · Cited by 27 · see citing works
Detection and characterization of betamethasone metabolites in human urine by LC‐MS/MS
Matabosch, X., Pozo, Ó. J., Monfort, N., Pérez‐Mañá, C., Farré, M., Segura, J., & Ventura, R. (2014) · Drug Testing and Analysis · doi:10.1002/dta.1770 · Cited by 27 · see citing works
de Jong, W. H. A., Buitenwerf, E., Pranger, A. T., Riphagen, I. J., Wolffenbuttel, B. H. R., Kerstens, M. N., & Kema, I. P. (2017) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2016-1072 · Cited by 29 · see citing works
Harding, B. N., Castaño‐Vinyals, G., Cros, A. P., Papantoniou, K., Espinosa, A., Skene, D. J., Middleton, B., Gomez‐Gómez, À., Navarrete, J. M., Such, P., et al. (2021) · Scandinavian Journal of Work Environment & Health · doi:10.5271/sjweh.3991 · Cited by 25 · see citing works
Urinary excretion profile of prednisone and prednisolone after different administration routes
Mazzarino, M., Piantadosi, C., Comunità, F., de la Torre, X., & Botrè, F. (2019) · Drug Testing and Analysis · doi:10.1002/dta.2733 · Cited by 23 · see citing works
Non-linear and non-additive associations between the pregnancy metabolome and birthweight
Colicino, E., Ferrari, F., Cowell, W., Niedzwiecki, M. M., Pedretti, N. F., Joshi, A., Wright, R. O., & Wright, R. J. (2021) · Environment International · doi:10.1016/j.envint.2021.106750 · Cited by 22 · see citing works
Quantitative Profiling of Cortisol Metabolites in Human Urine by High-Resolution Accurate-Mass Ms
Wang, Y., Fujioka, N., & Xing, C. (2018) · Bioanalysis · doi:10.4155/bio-2018-0182 · Cited by 22 · see citing works
Steroid profile analysis by LC-HRMS in human seminal fluid
Olesti, E., Garcia, A., Rahban, R., Rossier, M. F., Boccard, J., Nef, S., González‐Ruiz, V., & Rudaz, S. L. (2019) · Journal of Chromatography B · doi:10.1016/j.jchromb.2019.121929 · Cited by 22 · see citing works
de la Torre, X., Curcio, D., Colamonici, C., Molaioni, F., Cilia, M., & Botrè, F. (2015) · Drug Testing and Analysis · doi:10.1002/dta.1911 · Cited by 21 · see citing works
Xiang, Q., Shen, X., Li, K., Wang, Z., Zhao, X., & Chen, Q. (2023) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2023.167504 · Cited by 19 · see citing works
Mass spectrometric analysis of steroids: all that glitters is not gold
Lière, P., & Schumacher, M. (2015) · Expert Review of Endocrinology & Metabolism · doi:10.1586/17446651.2015.1063997 · Cited by 19 · see citing works
Honour, J. W., Conway, E., Hodkinson, R., & Lam, F. (2018) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2018.02.013 · Cited by 20 · see citing works
Grignon, C., Dupuis, A., Albouy-Llaty, M., Condylis, M., Barrier, L., Carato, P., Brunet, B., Migeot, V., & Venisse, N. (2017) · Journal of Chromatography B · doi:10.1016/j.jchromb.2017.07.013 · Cited by 18 · see citing works
Bioanalytical Techniques in Discrimination Between Therapeutic and Abusive Use of Drugs in Sport
Ventura, R., Matabosch, X., & Segura, J. (2016) · Bioanalysis · doi:10.4155/bio.15.253 · Cited by 17 · see citing works
Li, Y., Yan, Z., Li, X., Yin, X., & Li, K. (2021) · Molecules · doi:10.3390/molecules26206213 · Cited by 16 · see citing works
Luo, G., Li, Y., & Bao, J. J. (2016) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-015-9213-1 · Cited by 16 · see citing works
Adrenal hormonal imbalance in acute intermittent porphyria patients: results of a case control study
Pozo, Ó. J., Marcos, J., Fabregat, A., Ventura, R., Casals, G., Aguilera, P., Segura, J., & To‐Figueras, J. (2014) · Orphanet Journal of Rare Diseases · doi:10.1186/1750-1172-9-54 · Cited by 15 · see citing works
Yan, L., Rong, Q., Zhang, H., Jones, K. C., Li, Y., & Luo, J. H. (2022) · Environmental Science & Technology · doi:10.1021/acs.est.2c00834 · Cited by 14 · see citing works
Casals, G., Vizoso, M. A. B., Zamora, A., Martínez, I., Fernández‐Varo, G., Mora, M., Hanzu, F. A., & Morales‐Ruiz, M. (2024) · Biomolecules · doi:10.3390/biom14050558 · Cited by 13 · see citing works
Cortisol: Analytical and clinical determinants
Vega‐Beyhart, A., Castro, M. A., Hanzu, F. A., & Casals, G. (2022) · Advances in clinical chemistry · doi:10.1016/bs.acc.2022.11.005 · Cited by 12 · see citing works
Chen, C., Chao, Y.-Y., Lin, Y., & Chen, Y. (2018) · Journal of Chromatography A · doi:10.1016/j.chroma.2018.03.008 · Cited by 11 · see citing works
Urinary cysteinyl progestogens: Occurrence and origin
Marcos, J., Pol, M., Fabregat, A., Ventura, R., Renau, N., Hanzu, F. A., Casals, G., Marfà, S., Martín, B. B., Barceló, A., et al. (2015) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2015.04.015 · Cited by 11 · see citing works
Arregi, A., Robinson, O., Aasvang, G. M., Andrušaitytė, S., Dėdelė, A., Evandt, J., García‐Baquero, G., Krog, N. H., Guxens, M., Jaddoe, V. W. V., et al. (2025) · Environmental Research · doi:10.1016/j.envres.2025.121541 · Cited by 9 · see citing works
Fanjara, E., Aas, G. H., Cao, Y. W., Kristinová, V., Saeboe, A., & Stene, A. (2023) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2023.106401 · Cited by 9 · see citing works
Levina, I. S., Nazarov, A. K., Nazarov, G. V., Kulikova, L. E., Kuznetsov, Y. V., Dmitrenok, A. S., Minin, D. V., Aksenov, A. V., & Zavarzin, I. V. (2019) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2019.105436 · Cited by 8 · see citing works
Kiersztan, A., Nagalski, A., Nalepa, P., Tempes, A., Trojan, N., Usarek, M., & Jagielski, A. K. (2015) · Biochimie · doi:10.1016/j.biochi.2015.11.019 · Cited by 7 · see citing works
Associations of family affluence with cortisol production and telomere length in European children
Marston, K., Lau, C. E., Andrušaitytė, S., Bhopal, S., Gražulevičienė, R., Gützkow, K. B., Haro, N., Karachaliou, M., Koutra, K., Krog, N. H., et al. (2025) · EBioMedicine · doi:10.1016/j.ebiom.2025.105793 · Cited by 6 · see citing works
Honour, J. W. (2024) · Steroids · doi:10.1016/j.steroids.2024.109502 · Cited by 7 · see citing works
Xie, J., Wan, W., Yi, K., Qu, Z., Zhang, D., Huang, Z., Ye, Z., Dai, X., Jiang, Y., & Fang, X. (2023) · Analytical Letters · doi:10.1080/00032719.2023.2245078 · Cited by 4 · see citing works
A review on analytical methods of antigout agents
Kachave, R. N., Mandlik, P., & Wakchaure, S. R. (2020) · Journal of Reports in Pharmaceutical Sciences · doi:10.4103/jrptps.jrptps_32_19 · Cited by 3 · see citing works
Gomez‐Gómez, À., Montero-San-Martín, B., Haro, N., & Pozo, Ó. J. (2023) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2023.115586 · Cited by 3 · see citing works
Sampling time for different matrices in stress assessment of farmed Atlantic salmon post-smolt
Fanjara, E., Grete, K. F. H., Cao, Y., Kristinová, V., Sæbø, A., & Stene, A. (2024) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2024.106542 · Cited by 2 · see citing works
Nakaki, A., Gomez, Y., Darecka, K., Borràs, R., Vellvé, K., Paulés, C., Boutet, M. L., Basso, A., Casu, G., Traversi, P., et al. (2024) · Fetal Diagnosis and Therapy · doi:10.1159/000540580 · Cited by 2 · see citing works
Fabbri, L., Robinson, O., Basagaña, X., Chatzi, L., Gražulevičienė, R., Guxens, M., Kadawathagedara, M., Sakhi, A., Maitre, L., McEachan, R., et al. (2024) · Environmental Research · doi:10.1016/j.envres.2024.120413 · Cited by 4 · see citing works
Urinary Steroid Profiling in the Diagnosis of Adrenal Disorders
Honour, J. W. (2018) · Clinical Chemistry · doi:10.1373/clinchem.2017.285890 · Cited by 2 · see citing works
Pan, Y., Wang, Q., Chen, M., & Takao, T. (2024) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.9719 · Cited by 1 · see citing works
Jiao, Q., Zhao, Y., Li, Y., Liu, Y., Wang, H., Xu, Z., Chen, Q., & Shen, X. (2025) · Environmental Pollution · doi:10.1016/j.envpol.2025.126638 · Cited by 1 · see citing works
DUTCH (Dried Urine Test for Comprehensive Hormones) In Preventing Diseases Related Hormonal Function
Widuri, A., Fakhriani, R., Agustin, Q., & Cahyarani, A. (2022) · Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya · doi:10.30651/jqm.v6i2.9204
Quantitative analysis of steroids
Honour, J. W. (2023) · Elsevier eBooks · doi:10.1016/b978-0-12-818124-9.00010-3
Profiling of urinary steroids by lithium ion adduction-based UPLC-MS/MSPreprint
Takao, T., Pan, Y., Wang, Q., & Chen, M. (2023) · doi:10.22541/au.170024586.67826754/v1
Jiao, L., Shen, X., Tai, S., Chang, H. Z., & Wan, Y. (2026) · ACS ES&T Water · doi:10.1021/acsestwater.5c01445
Stéroïdomique corticosurrénalienne par LC-MS/MS : applications en endocrinologie pédiatrique
Travers, S. A. A. (2020) · theses.fr (ABES) · doi:10.70675/84cf9c3bze93ez41dcza251z559f27f3c6a6
- Authors
- Marcos, J., Renau, N., Casals, G., Segura, J., Ventura, R., & Pozo, O. J.
- Affiliations
- Universitat Pompeu Fabra (ES)
- Municipal Institute for Medical Research (ES)
- Hospital Clínic de Barcelona (ES)
- Venue
- Analytica Chimica Acta
- Publisher
- Elsevier BV
- Published
- 2014-02
- Volume
- 812
- Pages
- 92-104
- ISSN
- 0003-2670 (ISSN-L)
- OpenAlex
- W1998754189
- Cited by
- 76
- References
- 35
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 8 · 2023: 8 · 2022: 5 · 2021: 6 · 2020: 5 · 2019: 10 · 2018: 9 · 2017: 5 · 2016: 4 · 2015: 8 · 2014: 3
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytica Chimica Acta (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Instituto de Salud Carlos III
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- endogenous corticosteroids, urine, glucocorticoids, mineralocorticoids, progestins, steroid quantification, LC-MS/MS method validation, multiple reaction monitoring, β-glucuronidase hydrolysis, 24-hour urine collection, healthy volunteers, matrix effects
- MeSH terms
- Adrenal Cortex Hormones, Chromatography, Liquid, Humans, Reference Standards, Reproducibility of Results, Tandem Mass Spectrometry, Limit of Detection
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
