Cited by 101T-cell and B-cell ImmunologyPsoriasis: Treatment and PathogenesisDermatology and Skin Diseases

The TCR δ Repertoire in Normal Human Skin is Restricted and Distinct from the TCR δ Repertoire in the Peripheral Blood

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Holtmeier, Wolfgang, Pfänder, Michael, Hennemann, Andreas, Caspary, Wolfgang F., Zollner, Thomas M., Kaufmann, Roland·2001·Journal of Investigative Dermatology

Abstract

The skin and the intestinal mucosa form surfaces to external environments and share similarities in anatomic structure and immunologic defense. In healthy humans, intestinal gamma/delta T cells express a highly restricted gamma/delta T cell receptor repertoire whereas gamma/delta T cells of the skin were thought to express a polyclonal repertoire. Herein we report, using complementarity-determining region 3 size spectratyping and nucleotide sequencing of T cell receptor DV1 and DV2 rearrangements, that the human skin is also composed of clonally expanded gamma/delta T cells that are widely distributed. Identical complementarity-determining region 3 profiles and T cell receptor delta rearrangements were found in two separate skin samples that were obtained as far as 2-10 cm apart. Furthermore, analysis of peripheral blood mononuclear cells of these subjects clearly demonstrated that the skin harbors a unique population of gamma/delta T cells that is distinct from that in the peripheral blood. In addition comparable data were obtained irrespective of whether DNA or RNA was analyzed, indicating that the observed oligoclonality is not secondary to the expression of large amounts of mRNA from a few activated cells. Thus, gamma/delta T cells of the skin and the intestine both express an oligoclonal repertoire that enables them to respond to a variety of deleterious antigens without the need for diverse T cell receptors, possibly by recognition of stress-induced self-antigens or of conserved foreign antigens.

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Crossref · 10.1046/j.1523-1747.2001.01250.x

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  • IL-17 release by DETCs can promote DNA repair following exposure to UV radiation and protect the skin against potential opportunistic infections by releasing keratinocyte-derived antimicrobial peptides.

    “IL-17 release by DETCs can promote DNA repair following exposure to UV radiation and protect the skin against potential opportunistic infections by releasing keratinocyte-derived antimicrobial peptides (37, 38).”

    From Comparing Mouse and Human Tissue-Resident γδ T Cells (2022) · cites this paper as [37]

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Authors
Holtmeier, W., Pfänder, M., Hennemann, A., Caspary, W. F., Zollner, T. M., & Kaufmann, R.
Affiliations
Goethe University Frankfurt (DE)
Corresponding author
Holtmeier, Wolfgang
Venue
Journal of Investigative Dermatology
Publisher
Elsevier BV
Published
2001-02
Volume
116
Issue
2
Pages
275-280
ISSN
0022-202X (ISSN-L)
OpenAlex
W2045500037
Cited by
101
References
44
Citations by year
2026: 1 · 2025: 4 · 2024: 6 · 2023: 7 · 2022: 3 · 2021: 3 · 2020: 8 · 2019: 1 · 2018: 8 · 2017: 3 · 2016: 2 · 2015: 3 · 2014: 7 · 2013: 5 · 2012: 2
FWCI
1.41
Type
journal article
Language
en
Open access
Locations
  • Journal of Investigative Dermatology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
Keywords
γδ T cells, T cell receptor delta chain, skin, peripheral blood, clonal expansion, T-cell receptor gene rearrangement, skin immune system, intestinal mucosa
MeSH terms
Clone Cells, Humans, Skin, Receptors, Antigen, T-Cell, gamma-delta, Gene Expression Profiling, Complementarity Determining Regions

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